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陕西省调查处理输入性登革热病例的报告
...。 1.2.2使用美国Cortez公司的DengueIgM/IgG硒标试剂、德国IBL公司的DengueIgGELISA试剂和中国疾病预防控制中心提供的登革热病毒RNA引物，进行登革热特异性IgM、IgG抗体和病毒RNA扩增检测。 1.2.3媒介监测分别在陕西的西、中、东...
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海南省2005～2006年登革热血清流行病学调查
...剂检测登革IgM抗体，但2005年52份发热病人血样同时用德国IBL公司的ELISA试剂平行检测登革IgM和IgG抗体。 1.3数据分析个案调查资料和实验室结果录入SPSS11.5进行统计分析。 2结果0.05），既往高发区总的登革IgG抗体阳性率为1.9...
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Zincandpneumonia
...esult,noapparentexplanationwasforthcoming.Apharmacologiceffectofzincisplausible(5),but,asinthereportbyBoseetal,itiswidelyacceptedthatthebeneficialeffectsofzincsupplementsinthepreventionandtreatmentofdiarrheaandpneumoniaaremostlikelytobeduetothepreventionorcorrectionofzincdeficiency.Hence,beneficiale...
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UpregulatedExpressionof14-3-3ProteinsinAstrocytesFromHumanCerebrovascularIschemicLesions
...d1:400),arabbitpolyclonalanti-14-3-3antibody(Immuno-BiologicalLaboratories[IBL];diluted5μg/mL),andarabbitpolyclonalanti-14-3-3antibody(IBL;diluted2μg/mL).Theanti-14-3-3antibody(H-8)recognizesallhuman14-3-3isoforms,9,10whereastheotherantibodiesare14-3-3isoformspecific.10,11ImmunohistochemistryForma...
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肺结核患者血清和尿液新喋呤水平的变化
...并收集当天晨尿，保存于-20℃直至测定。NT测定采用德国IBL公司试剂盒（ELISA法），按照说明书操作步骤进行。为排除尿液浓缩的影响，尿液NT水平以尿NT水平与尿肌酐（Cr）水平的比值表示。 1.3数据处理采用SPSS11.0软件包。 2...
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肺炎衣原体慢性感染与冠心病的血清学相关性研究
...风湿因子（BF）吸附试验后重新检测（RF吸附液购自德国IBL公司）。 123诊断标准：①急性肺炎衣原体感染：双份血清抗体（IgG或IgM）滴度升高4倍及其以上；IgM≥1∶16；IgG≥1∶512。上述3条符合其中1条即可诊断。②既往感...
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肺炎衣原体慢性感染与冠心病的血清学相关性研究
...风湿因子（BF）吸附试验后重新检测（RF吸附液购自德国IBL公司）。 123诊断标准： ①急性肺炎衣原体感染：双份血清抗体（IgG或IgM）滴度升高4倍及其以上；IgM≥1∶16；IgG≥1∶512。上述3条符合其中1条即可诊断。②既...
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肺炎衣原体慢性感染与冠心病的血清学相关性研究
...风湿因子（BF）吸附试验后重新检测（RF吸附液购自德国IBL公司）。 123诊断标准：①急性肺炎衣原体感染：a双份血清抗体（IgG或IgM）滴度升高4倍及其以上；b.IgM≥1∶16；c.IgG≥1∶512。上述3条符合其中1条即可诊断。②...
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深圳宝安区2～6岁儿童水痘疫苗免疫成功率监测
...所有标本随机编号后，送宝安区CDC检验科检验，采用德国IBL公司的水痘-带状疱疹病毒IgG抗体检测试剂及按照说明书操作步骤（ELISA法）检测水痘IgG抗体，以8U/ml为阴性，8U/ml为阳性。 2结果 检测147人，其中免疫前或后不合...
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2006年深圳市宝安区麻疹人群免疫水平调查
...抗体浓度≥10ng/ml为阳性,≤10ng/ml为阴性。检验试剂德国IBL公司生产的MeaslesVirusIgGELISA试剂，批号为RE56611，有效期为2006年10月6日，在有效期内使用。2结果7个年龄组共监测352例，总阳性率为84.09%。其中男181例，抗体阳性的有144例...
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ThyroidAutoimmunityandSpontaneousCervicalArteryDissection
...antidouble-strandeddeoxyribonucleicacidantibodiesbyradioimmunosorbentassay(IBL);antiextractablenuclearantigenantibodiesbyenzyme-linkedimmunosorbentassay(Innogenetics);rheumatoidfactorandC3toC4complementfractionbynephelometry(DadeBehring);andcryoglobulinsbystandardprocedure.Insubjectswithraisedlevels...
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不同年龄男性胃癌患者雄激素水平分析
...0。性激素结合球蛋白(SHBG)采用ELISA法测定，试剂盒系德国IBL公司产品，批内CV为5.5,批间CV为9.0。游离睾酮根据Vermeulen公式计算，睾酮分泌指数(TSI)=总睾酮／LH(nmol/IU)，游离睾酮指数(FTI)=总睾酮/SHBG(nmol/nmo1)。标本的测定由同一检测...
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EtiologyofMumps-LikeIllnessesinChildrenandAdolescentsVaccinatedforMeasles,Mumps,andRubella
...tyCentralHospital,HospitalforChildrenandAdolescents,Helsinki,FinlandThepossibleviraletiologyofmumps-likeillnessesinpatientsvaccinatedformeasles,mumps,andrubella(MMR)wasstudiedbyuseofserumsamplesprospectivelycollected,during19831998,from601acutelyillFinnishchildrenandadolescentswithmumps-likesymptoms...
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儿童风湿病患者肺炎支原体和EB病毒感染的研究
...IgA的测定全病毒EBV-IgM、IgA测定采用ELISA，试剂盒购自德国IBL公司（批号为RE56211），操作步骤按常规进行，判断标准CUTOFF为阳性。 2结果 检测结果见表1、表2、表3。 表1风湿病组与对照组血清MP-IgM、IgA，EBV-IgM、IgA阳性...
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降钙素基因相关肽对大鼠急性肺损伤的治疗作用及其可能机制
...码】A【文章编号】1684-2030(2010)03-0129-04 Effectandpossiblemechanismofcalcitoningene-relatedpeptideonacutelunginjury LIBei-hua,LIUHui-lin,LIUTao.Hospitalof69250PLATroops,Urumchi830013,China 【Abstract】ObjectiveTostudytheprotectedeffectofcalcitoningene-relatedpeptide(...
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瑞芬太尼不同靶浓度与全血实测浓度的差异以及对血流动力学的影响
...，注入肝素抗凝管，离心后分离血清，-20℃保存，用德国IBL公司试剂盒，根据邹远高等［5］报道高效液相色谱-电化学法测定E和NE的含量。 1.5统计学方法采用SPSS10.0统计软件包，计量资料以±s表示，二组间比较采用t检验，检...
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Effectsofdietarysaturated,monounsaturated,andn–3fattyacidsonbloodpressureinhealthysubjects
...unction,hemostaticfactors,andmicroalbuminuria.Geneticfactorsseemtoberesponsibleforasmuchas20–40%ofBPvariationsinthegeneralpopulation(9).However,epidemiologicdataimplicatethatlifestylefactors(eg,dietaryhabits)areamajorcontributortothehighprevalenceofhypertension(10).Nevertheless,ourknowledgeofth...
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CharacterizationofMycobacteriumcapraeIsolatesfromEuropebyMycobacterialInterspersedRepetitiveUnitGenotyping
...ctivebandsizewascalculatedaccordingtoinformationprovidedbyPhilipSupply(www.ibl.fr/mirus/mirus.html)andenteredintoaspreadsheetfile.AtBorstel,MIRUcodesweredeterminedbyT.K.andS.N.bymultiplex-PCRMIRUtypingaccordingtoapreviouslydescribedmethod(45).SpoligotypingandIS6110RFLPanalysis.Spoligotypingwascarrie...
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中医药治疗宫颈癌研究进展
...清扫加经阴道根治性宫颈切除尤其适宜有生育要求的Ia2～Ibl期且局部肿瘤直径小于2cm的早期宫颈癌患者。但其中也存在着一些亟待解决的问题。诸如根治性子宫切除术可能会影响盆腔自主神经的功能甚至影响生殖及性功能，而盆...
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单核细胞激活及Th1/Th2细胞因子的变化与肾综合征出血热病情的关系
...IL-12、IFN-γ均采用ELISA方法检测，试剂盒分别购自德国IBL公司、比利时Biosource公司和法国Diaclone公司（IFNγ）；IL-10用放射免疫法（RIA）检测，试剂盒购自解放军总医院科技开发中心放免所。为避免反复冻融而影响检测结果...
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Dietaryglycemicloadandriskofage-relatedcataract
...aselinesemiquantitativefood-frequencyquestionnaire(SFFQ),thosewhohadimplausiblecaloricintakes(600or3500kcal/dforwomenand800or4200kcal/dformen),andthosewholeft70itemsblankontheSFFQ;atotalof82126womenand49934menwerethuspotentiallyavailableforanalysis.Fromthesegroups,wefurtherexcludedallparticipantswho...
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EvidenceforaroleofproteinkinaseC-inurineconcentration
...usealbumin(CellTrend,Luckenwalde,Germany)oracommercialRIAkitforvasopressin(IBL,Hamburg,Germany),respectively.Afterurinecollectionwascompleted,themicewereanesthetizedwithetherandbloodwasdrawnfromtheretrobulbarplexusfordeterminationofhematocritandplasmaosmolality.Twenty-four-hoururinecollectionswerepe...
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RedistributionofvitaminAafterironsupplementationinIndonesianinfants
...f0.05andapowerof0.90.Toallowfordropouts,atargetof60infants/groupwasset.Eligibleinfantswereidentifiedbyvillagehealthvolunteers,andthemotherswereinvitedtoparticipateinthestudy.Motherswereinformedoftheproceduresandpurposeofthestudy.Afterwritteninformedconsentwasgivenbythemother,infantswereassessedanthr...
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AblationoftheSympatheticNervousSystemDecreasesGram-NegativeandIncreasesGram-PositiveBacterialDissemination:KeyRolesforTumorNecrosisFactor/Ph
...100,and1000cfuofP.aeruginosaorS.aureus(ATCC5638)/mL;10cfu/mLyieldedreproducibledatawiththesmallestcoefficientofvariation.Thisamountwasthenusedinallexperimentsshown(theuseofmorebacteriaresultedinbacterialovergrowthandthedestructionofcells).Supernatantwascollected,sodiumazidewasaddedtostopbacterialgro...
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DualMechanismsRegulatingAMPKKinaseActionintheIschemicHeart
...tingofancatalyticsubunitandandregulatorysubunits.TheprimarymechanismresponsibleforAMPKactivationinvolvesphosphorylationoftheThr172residuelocatedwithintheactivationloopofthecatalyticsubunit.9Additionalphosphorylationsiteshavebeenidentifiedontheandsubunits,buttheirfunctionalrolesremainuncertain.10,11A...
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Stimulationofsolubleguanylylcyclaseinhibitsmesangialcellproliferationandmatrixaccumulationinexperimentalglomerulonephritis
...asurementofcGMPwasperformedintriplicateusingacommercialradioimmunoassaykit(IBL,Hamburg,Germany)asdescribedbefore(33).Westernblotanalysis.Proteinsamplescontaining20μgofproteinfromisolatedglomeruliwereseparatedby8%SDS-PAGEandblottedontoPVDFmembranes.Blotswereblockedfor12hat4°Cinblockingsolutionconta...
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TheEffectofHydroxylamineonKATPChannelsinVascularSmoothMuscleandUnderlyingMechanisms
...esentericarteriesandunderlyingmechanisms.Itwasfoundthatbath-appliedHAreversiblyenhancedKATPcurrentsinaconcentration-dependentfashionwithanEC50of54±3.4eandhyperpolarizedthecellmembranefromeC48±5.2toeC65±7.5mV(n=6,p0.01).TheincreaseinKATPcurrentsinducedbyHAwassuppressedbysuperoxidedismutase(eC380±...
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Zincplusß-carotenesupplementationofpregnantwomenissuperiortoß-carotenesupplementationaloneinimprovingvitaminAstatusinbothmothers
...ingasinglehigh-doseofvitaminAtothemotherdirectlypostpartum.BecauseofthepossibleteratogenicityofvitaminAinhumans(7),theWorldHealthOrganizationrecommendsthatonlysmalldoses(10000IU/dor25000IU/wk)shouldbegiventomothersduringpregnancy(8).Similarly,onlysmalldoses(3dosesof50000IUwithintervalsofatleast1mo)o...
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AnalysisofMycobacteriumtuberculosisGenotypesinMadridandIdentificationofTwoNewFamiliesSpecifictoSpain-RelatedSettings
...ulatedbymeansofthereferencesincludedintheMIRUprovisionalwebpage(http://www.ibl.fr/mirus/mirus.html).TheMIRUtypeconsistsofa12-numbercodethatindicatesthenumberoftandemrepeatsfoundforeachoftheMIRUloci.PhylogeneticcomparisonsbetweengenotypeswereperformedusingBionumericsversion3.0(AppliedMaths,St.Marten-...
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Acyl-CoenzymeA:CholesterolAcyltransferase-2(ACAT-2)IsResponsibleforElevatedIntestinalACATActivityinDiabeticRats
...hatincontrolrats.Conclusions—OurdatademonstratethatACAT-2isozymeisresponsiblefortheincreasedintestinalACATactivityofdiabeticrats,suggestinganimportantroleofACAT-2fordyslipidemiaindiabeticpatients.Diabeticratsexhibitdyslipidemiacaused,inpart,byelevatedintestinalacyl-coenzymeA:cholesterolacyltransfe...