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AngiotensinIICa2+signalinginratafferentarterioles:stimulationofcyclicADPriboseandIP3pathways
...edtheANGII-inducedincreasesin[Ca2+]iby75and69%,respectively.NicotinamideandZn2+areknowninhibitorsoftheVSMADPRcyclase.Nicotinamidediminishedthe[Ca2+]iresponsetoANGIIby66%.Incalcium-freebuffer,Zn2+reducedtheANGIIresponseby68%.SimultaneousblockadeoftheIP3andcADPRpathwaysdiminishedthe[Ca2+]iresponsetoAN...
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泰脂安胶囊治疗高脂血症50例临床观察
...pwas70.12%and85.71%separately.ThelevelofIgG、IgE、ComplementC3andelementsZn、Fe、Cu、Mg、Pshowednodifferencestatistically(P＞0.05).ButtheserumIgGandIgElevelsinbothgroupsweresignificantlyhigherthanthoseincontrolgroup(P＜0.01).TherewasnosignificantdifferenceatthecomplementC3betweenbothgroupsan...
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Long-termmoderatezincsupplementationincreasesexchangeablezincpoolmassesinlate-middle-agedmen:theZenithStudy
...otopezinckineticstudyafter6moofdailysupplementationwith0(placebo),15,or30mgZn.Attheendofthesupplementationperiod,eachsubjectreceivedanintravenousinjectionof0.89mg70Zn,andtheplasmazincdisappearancecurvewasmonitoredforthenext10d.Twoapproacheswereusedtodeterminethecharacteristicsoftheexchangeablezincpo...
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InhibitionofapoptosisbyZn2+inrenaltubularcellsfollowingATPdepletion
...nizeapoptosis.However,apoptosisinhibitoryapproachesremaintobefullyexplored.Zn2+haslongbeenimplicatedinapoptosisinhibition;butsystematicanalysisoftheinhibitoryeffectsofZn2+islacking.Moreover,whetherZn2+blocksrenalcellapoptosisfollowingischemiaisunknown.Here,wedemonstratethatZn2+isapotentapoptosisinhi...
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Acutestudyofinteractionamongcadmium,calcium,andzinctransportalongtheratnephroninvivo
...ro;M)intoxicationonrenalfunctionandcharacterizesthetransportofCa2+,Cd2+,andZn2+intheproximaltubule(PT),loopofHenle(LH),andterminalsegmentsofthenephron(DT)usingwholekidneyclearanceandnephronmicroinjectiontechniques.AcuteCd2+injectionresultedinrenallossesofNa+,K+,Ca2+,Mg2+,PO4-2,andwater,buttheglomeru...
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Thequalityofreportingofdiagnosticaccuracystudiespublishedinophthalmicjournals
...oAzuara-BlancoPhDFRCS(Ed),TheEyeClinic,AberdeenRoyalInfirmary,AberdeenAB252ZN,UK;aazblanco@aol.comAcceptedforpublication8August2004ABSTRACTAim:Toevaluatethequalityofreportingofalldiagnosticstudiespublishedinfivemajorophthalmicjournalsintheyear2002usingtheStandardsforReportingofDiagnosticAccuracy(STA...
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Cu-PTSM:一种用于PET显像的药物
...的合成用Petering等［1］的方法可以合成Cu-PTSM。还可以从62Zn/62Cu发生器中制备。62Cu合适的半衰期和100%的正电子衰变与使用中等能量的回旋加速器生产母体核素效率高，价廉，便于运输。母体核素的制备：用能量范围是30或大于19Me...
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Adenovirus-MediatedExpressionofHumanParaoxonase3ProtectsAgainsttheProgressionofAtherosclerosisinApolipoproteinE–DeficientMice
...hatoverexpressionoftheintracellularantioxidantenzymescatalaseorcatalase+Cu/Zn-superoxidedismutase(SOD)ledtotheretardationofatheromadevelopmentinapoE–/–mice.24Inaddition,miceoverexpressingcatalaseorcatalase+Cu/Zn-SODexhibitedsignificantlylowerlevelsofF2-isoprostanesintheplasmaandaortarela...
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SerotoninInhibitsNeuronalExcitabilitybyActivatingTwo-PoreDomainK+ChannelsintheEntorhinalCortex
...=7).B,applicationofantagoniststoTASK-1,anandamide(10µM,n=6),orTASK-3,Zn2+(100µM,n=7),rutheniumred(RR,10µM,n=9)orcoapplicationofanandamideandrutheniumred(n=6)failedtochange5-HT-mediatedincreaseinoutwardholdingcurrent.C,applicationof5-HT(1µM)inducedaninwardholdingcurrentinHEK29...
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丙型肝炎病毒蛋白酶生物学功能研究进展
...生物学功能的成熟蛋白。研究表明，病毒编码的蛋白酶-Zn2+依赖性金属蛋白酶和丝氨酸蛋白酶与HCV非结构区的加工有密切关系。本文就这两种病毒蛋白酶结构、生物学功能、底物特异性及酶活性影响因素等作一综述。 HCV是输...
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厄贝沙坦对糖尿病大鼠肾脏MMP-2/TIMP-2基因表达的影响
...酶组织抑制物TIMPs可抑制MMPs的活性［4］。MMPs是一类含有Zn2+，活性依赖Ca2+的结构高度同源的内肽酶的总称，是目前认为最重要的一类降解ECM的蛋白酶类，几乎能降解全部ECM成分。 在本研究中，RT-PCR结果提示8周糖尿病大鼠肾...
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母血常、微量元素与胎儿宫内发育迟缓关系的研究
...IUGR组21例，对照组35例，巨大儿组15例。结果IUGR组母血清Zn、Ｓe、Ｍn、Ｆe、Ｍg、Ｃr明显低于对照组(P＜005)；Cd、Ｐb、Ｎi水平明显高于对照组及巨大儿组；其中，巨大儿组母血清Fe含量亦明显低于对照组(Ｐ＜005)；Ca、Ｔi、...
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母血常、微量元素与胎儿宫内发育迟缓关系的研究
...IUGR组21例，对照组35例，巨大儿组15例。结果IUGR组母血清Zn、Ｓe、Ｍn、Ｆe、Ｍg、Ｃr明显低于对照组(P＜005)；Cd、Ｐb、Ｎi水平明显高于对照组及巨大儿组；其中，巨大儿组母血清Fe含量亦明显低于对照组(Ｐ＜005)；Ca、Ｔi、...
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Randomizedcontrolledtrialoftheeffectofdailysupplementationwithzincormultiplemicronutrientsonthemorbidity,growth,andmicronutrientstatusof
...tentdiarrheawererandomlyassignedto1of3groupstoreceiveadailysupplementof10mgZnalone(Zn;n=81),zincplusvitaminsandothermineralsat1-2timesrecommendeddailyintakes(Zn+VM;n=82),orplacebo(n=83)for6moafterthediarrheaepisodeended.Morbidityinformationwascollectedonweekdays.Weight,length,andotheranthropometrici...
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Endothelium-DerivedHydrogenPeroxideAccountsfortheEnhancingEffectofanAngiotensin-ConvertingEnzymeInhibitoronEndothelium-DerivedHyperpolarizingF
...13coronaryarteries.Furthermore,wehaverecentlydemonstratedthatendothelialCu/Zn-superoxidedismutase(SOD)playsapivotalroletoproduceH2O2asan“EDHFsynthase“inmice.14Angiotensin-convertingenzyme(ACE)inhibitorsareknowntoimproveendothelialdysfunctionindiabetesmellitus,15aging,16andpatientswithatherogenic...
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InhibitionoftheCerebralIschemicInjurybyEthylPyruvateWithaWideTherapeuticWindow
...Themajorpathogenicmechanismsofthiscascademayincludeglutamateexcitotoxicity,Zn+2toxicity,peri-infarctdepolarization,inflammation,andapoptosis.ExcitotoxicityandZn+2toxicityresultinacuteandmassiveneuronaldeathintheischemiccore.1Thereisawealthofevidencetosuggestthatthisacuteneuronaldamageisfollowedbyase...
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第二节 电极电位
...位 一、原电池 将锌片插入CuSO4溶液中，锌片上的Zn原子失去电子成为Zn2+而溶解；溶液中的Cu2+得到电子成为金属Cu在锌片上析出，即发生如下的氧化还原反应： 反应中电子从锌原子转移给铜离子。由于锌片和硫酸铜溶...
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UncouplingofEndothelialNitricOxidaseSynthasebyHypochlorousAcid
...enovirusInfectionConfluentHUVECswereinfectedwitheitheradenovirusencodingCu/ZnSOD(SOD-1),p67phoxdominantnegative(p67phox-DN),orPKC-dominantnegative(PKC--DN),asdescribedpreviously.21Adenovirusencodinggreenfluorescenceprotein(GFP)wasusedasacontrol.AssayofNitricOxideSynthaseActivityTheNOsynthesizingacti...
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MicroparticlesofHumanAtheroscleroticPlaquesEnhancetheSheddingoftheTumorNecrosisFactor-ConvertingEnzyme/ADAMSubstratesTumorNecrosisFactorand
...;lofthefinalactivitybuffer(25mmol/LTris/HCl,pH8.0,containing2.5µmol/LZnCl2).FluorogenicpeptideI(substrateofMMP-1,-2,-7,-8,-9,-12,-13,-14,-15,and-16),peptideII(substrateofMMP-3and-10)(R&DSystems),andpeptideIIIweredilutedintheactivitybufferatthefinalconcentrationof10µmol/L.Recombinantectod...
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妊娠不良结局与微量元素相关性研究
...icro-elements.MethodsLevelsofmicro-elements，includingSe，Mn，Fe，CuandZnweremeasured.Studygroupswereincludingthreatenedabortiongroup（n=134），prematureruptureofmembrane（n=31）andpretermlaborgroup（n=35），controlgroup（n=244）.ResultsThereweremarkedchangesinallstudygroups（P<0.01...
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Folicacidenrichmentofbreaddoesnotappeartoaffectzincabsorptioninyoungwomen
...Theservingsofbread(100g)differedinzincandfolicacidcontentsasfollows:A,1.2mgZnand17µgfolicacid;B,1.2mgZnand144µgfolicacid;C,3.0mgZnand17µgfolicacid;andD,2.9mgZnand144µgfolicacid.Mealswereextrinsicallylabeledwith65Znandabsorptionwasestimatedfromwhole-bodyretentionmeasurements.Folat...
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Photodynamictherapyforinflammatorychoroidalneovascularisationunresponsivetoimmunosuppression
...hthalmologyDepartment,AberdeenUniversityHospital,Foresterhill,AberdeenAB252ZN,UK;noemilois@aol.comAcceptedforpublication21July2004ABSTRACTAim:Toreportonvisualandangiographicoutcomesofaconsecutiveseriesofpatientswithinflammatorychoroidalneovascularmembranes(CNV)unresponsivetosystemicimmunosuppression...
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UpregulationofVascularExtracellularSuperoxideDismutaseinPatientsWithAcuteCoronarySyndromes
...oxygen.4–6ThreedifferentisoformsofSODhavebeendescribed.Copper/zincSOD(Cu/Zn-SOD)7andmanganeseSOD(Mn-SOD)8areintracellularlypresentinthecytosolandmitochondria,respectively.Bycontrast,extracellularSOD(EC-SOD)9localizesinextracellularspacebybindingtoheparansulfateproteoglycansintheinterstitialmatrixa...
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电化学疗法中阵列电极的结构与场分析
...z1）（z-z2）（z-z3）（z-z4）（z-z5）（z-z6）+邋6$n=1k=1Ank（z-zn）-k。其中zn=bei5-n3p（n=1～6），zn是电极中心所在位置的复数表示，Ank为第n根电极的k阶系数，因为b?a电位在组织中的分布随k的增大而衰减得很快，取零阶就可以得到较好...
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ImprovedSensitivityofSputumSmearMicroscopyafterProcessingSpecimenswithC18-CarboxypropylbetaineToDetectAcid-FastBacilli:aStudyofUnitedStates
...s.SpecimenswereexaminedbydirectstainingbyuseofbothauramineandZiehl-Neelsen(ZN)stainingtechniquesaccordingtorecommendedprocedures(7).Afterstaining,equalvolumesof50mMNaOHcontaining0.5%N-acetyl-L-cysteine(NALC)wereusedtohomogenizethespecimens,andspecimensweresplitapproximatelyequally.Onehalfofeachspeci...
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Ironsupplementsinhibitzincbutnotcopperabsorptioninvivoinileostomysubjects
...±9y]ingestedastable-isotope-labeledzincandcoppersolutioncontaining12mgZn(66Znand67Zn)and3mgCu(65Cu)inthepresenceof0,100,or400mgFeasferrousgluconateon3respectivetestdays.Subsequently,1mg70Znwasinjectedintravenously.Subjectscollectedileostomyeffluentandurinefor24hand7d,respectively.Zincstatusandt...
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Targetingtheheme-oxidizednitricoxidereceptorforselectivevasodilatationofdiseasedbloodvessels
...-activatingmetalloporphyrins:BAY58-2667–stimulatedsGCactivitywasblockedbyZn-,Mn-,andFe2+-protoporphyrininacompetitivemanner(Figure2C).Moreover,inaradioligandbindingstudy,sGC-bound,3H-labeledBAY58-2667wascompletelyreplacedbyZn-PPIX(Figure2D;IC50,20±1.3nM).ThisresultstronglysuggeststhatBAY58-2667an...
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InsulinIncreasesthePotencyofGlycineatIonotropicGlycineReceptors
...igma-Aldrich)werepreparedinacidifiedwater(HCl,pH2).StocksolutionscontainingZnCl2andtricine(bothfromSigma-Aldrich)werepreparedinwater.Forexperimentsthatinvestigatedtheroleoftheinsulinreceptorandsecond-messengersystems,HNMPA,lavendustinA,lavendustinB(allfromCalbiochem,LaJolla,CA),andwortmannin(Sigma-A...
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DiagnosisofExtrapulmonaryTuberculosisbySmear,Culture,andPCRUsingUniversalSampleProcessingTechnology
...analyzedbyconventionalmicrobiology,includingsmearmicroscopybyZiehl-Neelsen(ZN)stainingandcultureasdescribedbelowandcytological(ZNandGiemsastaining)andbiochemical(proteinandglucose)examinations.Anequalvolumewasstoredat–20°CforprocessingbytheUSPmethodandsubsequentZNstaining,cultureforM.tuberculosis...
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中药品种的计算机鉴别
...用星区图法准确区别浙贝和川贝母；（2）对Fe、Mn、Cu、Zn、Ba、Sr、Cr、Co、Ni等特征进行灰色关联度分析，发现浙贝母、川贝母微量元素关联序完全不同，进一步采用元素含量均值偏移度法，对浙贝母和川贝母进行鉴别分类。 ...