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InteractionofthemetalchelatorDMPSwithOAT1andOAT3inintactisolatedrabbitrenalproximaltubules
...oftheDMPSinteractionwithOAT1wereshowntobepresentinisolatedS2segmentsofrabbitRPT,includingtheobservationthatDMPScantrans-stimulatetheeffluxoffluorescein,amodelsubstrateforOAT1,fromRPTcells(3).Becauseorganicaniontransportinintactproximaltubulesreflectsthenetactivityofallassociatedtransportprocesses,th...
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FunctionalmapofTEAtransportactivityinisolatedrabbitrenalproximaltubules
...presentstudy,weextendthe“functionalmapping“ofOCtransportactivityinrabbitRPTtotheearlyandlateportionsofthetubule,i.e.,theS1andS3segments.TheS1segmentdisplayedaprofileofOCTactivitythatwasvirtuallytheoppositeofthatexpressedintheS2segmentinthatTEAtransportwasdominatedbyaninteractionwithOCT1,ratherth...
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RelativecontributionofOATandOCTtransporterstoorganicelectrolytetransportinrabbitproximaltubule
...ionwithPAH(IC501mM).Incontrast,bothtransportersrobustlyaccumulatedOTA.IntactRPTalsoaccumulatedOTA,withOAT1andOAT3eachresponsiblefor50%:ESandPAHeachreduceduptakeby50%,andthecombinationofthetwoeliminatedmediatedOTAuptake.TheweakbaseCIMwastransportedbyOAT3(Ktof80µM)andOCT2(Ktof2µM);OCT1hada...