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InVivoResponsivenesstoEzetimibeCorrelateswithNiemann-PickC1Like-1(NPC1L1)BindingAffinity:ComparisonofMultipleSpeciesNPC1L1Orthologs
...rollevelsbyblockingintestinalabsorptionofcholesterol.Ezetimibe(Zetia;Merck/Schering-Plough,Kenilworth,NJ),thefirst-in-classrepresentativeofthe2-azetidinones,blocksbothdietaryandbiliarycholesterolabsorptionintheproximaljejunuminhamsters(Salisburyetal.,1995).Ezetimibeadministrationalsopreventsthedevel...
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一种新型HCV蛋白酶抑制剂能有效抑制丙型肝
...月期发表的一篇文章，一种新的口服型HCV蛋白酶抑制剂（SCH503034）有很好的耐受性并且可能能够有效抑制丙型肝炎病毒的1型基因，这种基因在干扰素治疗时很难被破坏。研究者表示，SCH503034是NS3蛋白酶的一种特异的抑制剂，而NS...
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电刺激大鼠腹侧被盖区建立精神分裂症动物模型
...，并给予不同剂量的多巴胺受体拮抗剂氟哌啶醇（HAL）和SCH23390及促多巴胺释放剂苯丙胺，观察它们对大鼠精神分裂症模型的影响。结果刺激VTA可引发大鼠的异常行为，HAL可提高VTA刺激阈值，降低其行为评分;SCH23390可提高大鼠VTA...
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左旋多巴诱发异动症大鼠基底节环路功能改变的研究
...节环路功能改变与异动症形成之间的关系。研究者分别以SCH23390（D1受体拮抗剂）、氟哌啶醇（D2受体拮抗剂）治疗LID大鼠,观察LID大鼠的行为学改变,并用逆转录聚合酶链反应技术检测其纹状体区PDynmRNA和NGFI-BmRNA表达的变化。结果L...
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小儿过敏性紫癜的心肌损伤
...紫癜过敏性血管炎心肌损伤MyocardialinjuryinchildrenwithHenoch-SchnleinpurpuraSHAOZhu-cheng.TheSecondHospitalofZoucheng,Shandong273500,China.【Abstract】ObjectiveToinvestigatetheincidenceandclinicalcharacteristicsofHenoch-Schnleinpurpura（HSP）withmyocardialinjury.MethodsNinty-th...
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亚临床甲状腺功能减退与血脂及心脑血管疾病关系的研究
...脑血管疾病亚临床甲状腺功能减退症(SubclinicalHypothyroidism,SCH)是指血清促甲状腺激素(thyroid-stimulatinghormone,TSH）水平升高，血清游离甲状腺素（FreeFhyroxine，FT4）和游离三碘甲状腺原氨酸（FreeTriiodothyronine，FT3）水平正常，患者没有...
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Agonist-InducedCellSurfaceTraffickingofanIntracellularlySequesteredD1DopamineReceptorHomo-Oligomer
...andtreatmentofvariousneuropsychiatricandneurodegenerativedisordersincludingschizophrenia(LidowandGoldman-Rakic,1994;Okuboetal.,1997),cocaineaddiction(HummelandUnterwald,2002),andParkinson‘sdisease(Emilienetal.,1999).WehavepreviouslyshownthatD1dopaminereceptorsexistashomo-oligomers(Ngetal.,1994;Geo...
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DopaminerecruitsD1AreceptorstoNa-K-ATPase-richcaveolarplasmamembranesinratrenalproximaltubules
...presentinintracellularcompartmentsunderbasalconditions.Weobserved,using[3H]SCH-23390bindingandimmunoblotting,thatdopaminerecruitsD1Areceptorstotheplasmamembraneinratrenalproximaltubules.Furthermore,radioligandbindingand/orimmunoblottingexperimentsusingpharmacologicalmodulatorsshowedthatdopamine-indu...
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TheCCR5Receptor-BasedMechanismofActionof873140,aPotentAllostericNoncompetitiveHIVEntryInhibitor
...idinyl)carbonyl]-4‘-methyl-1,4‘-bipiperidin-4-yl}methanoneO-ethyloxime(Sch-C;SCH351125),4,6-dimethyl-5-{[4-methyl-4-((3S)-3-methyl-4-{(1R)-2-(methyloxy)-1-[4-(trifluoromethyl)phenyl]ethyl}-1-piperazinyl)-1-piperidinyl]carbonyl}pyrimidine(Sch-D;SCH417,690),4,4-difluoro-N-((1S)-3-{(3-endo)-3-[3-me...
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InvolvementofDopamineSysteminRegulationofNa+,K+-ATPaseintheStriatumuponActivationofOpioidReceptorsbyMorphine
...hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepinehydrochloride(SCH23390).However,bothshort-termandlong-termmorphinetreatment-inducedchangesinNa+,K+-ATPaseactivitycouldbereversedbyopioidreceptorantagonistnaltrexone.ItwasfurtherfoundthatcAMP-dependentproteinkinase(PKA)wascruciallyinvolvedi...
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抗幽门螺杆菌单克隆抗体的制备和临床应用
...规鉴定为Hp菌株，细菌悬液涂片，丙酮固定60min后加Hp－mAbSCH9（1：100稀释），过夜。洗涤后加辣根过氧化酶标记兔抗鼠IgG（1：250稀释），室温1h，洗涤后二氨基联苯显色。蒸馏水冲洗终止反应。结果判断：（－）Hp未染色；（＋...
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大鼠心脏多巴胺1受体与心肌肥厚的关系
...动脉狭窄+倍他乐克组（A+B组）；腹主动脉狭窄+倍他乐克+SCH23390组(A+B+S组)；假手术+倍他乐克+FODA组（B+F组）。各组均于4周后测定大鼠心功能和左心室/体重比值（LVW/BW）；另外还测定了A组心肌肥厚大鼠的心肌组织在FODA作用后cAMP...
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ReceptorRegulationofGeneExpressionofAxonGuidanceMolecules:ImplicationsforAdaptation
...apseformation,cellmigrationandaxonguidance(Zhou,1998;Klein,2001;KnollandDrescher,2002).Axonguidancemoleculesarealsoexpressedinadultbrainandmaycontributetosynapticfunction,neuroadaptation,andresponsetobraininjury(Gerlai,2001;Hafneretal.,2004;YamaguchiandPasquale,2004;Xiaoetal.,2006).Inthecontextofsyn...
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PNAS：昆虫体色的改变有时会致命
...蚂蚁，故称蚁蚕。正常蚁蚕体色为黑色，一种伴性赤蚁（sch，sex-linkedchocolate）突变，纯合型具有巧克力色的体色。由于该基因位于家蚕Z染色体上，所以杂合型只有雌蚕变成了巧克力色，而雄蚕仍然为黑色。然而，雌蚕“美丽变...
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TheTwo-StateDimerReceptorModel:AGeneralModelforReceptorDimers
...emicalsInc.,Brea,CA)orfrom0.09to10.5nMoftheD1dopaminereceptorantagonist[3H]SCH23390(85Ci/mmol;PerkinElmerLifeandAnalyticalSciences,Boston,MA)at25°Cin50mMTris-HClbuffer,pH7.4,containing10mMMgClandfor[3H](R)-PIAbinding,0.2U/mladenosinedeaminase(EC3.5.4.4[EC];RocheDiagnosticsS.L.,SantCugatdelVall&e...
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CD40Ligand
...Medicine,DukeUniversityMedicalCenter,Durham,NC.CorrespondencetoPascalJ.Goldschmidt-Clermont,DivisionofCardiology,DepartmentofMedicine,DukeUniversityMedicalCenter,DUMC3703,Durham,NC27710.E-mailGolds017@mc.duke.eduKeyWords:atherosclerosis•inflammation•CD40ligand•stemcells•endot...
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西南大学PNAS文章：昆虫中一种致命的色彩
...蚂蚁，故称蚁蚕。正常蚁蚕体色为黑色，一种伴性赤蚁（sch，sex-linkedchocolate）突变，纯合型具有巧克力色的体色（图）。由于该基因位于家蚕Z染色体上，所以杂合型只有雌蚕变成了巧克力色，而雄蚕仍然为黑色。然而，雌蚕“...
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新的抗血小板治疗可降低PCI并发症和出血的危险性
...照的试验，目的是评价新型口服凝血酶受体拮抗剂（TRA）SCH530348在1,030例NorthAmerica和Europe.患者中的应用。TRA是一个阻断凝血酶对血小板作用的新型药物。1,030例患者以3:1的比例被随机分至10、20或40mg负荷剂量的SCH530348组或安慰剂...
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破解乙肝丙肝治疗之惑——美国肝病研究协会第56届年会一瞥
...二醇化干扰素无应答患者采用一种口服丙肝蛋白酶抑制剂SCH503034治疗。在第一项研究中，研究人员报告了应用SCH503034对原先聚乙烯二醇化干扰素治疗无应答的基因1型HCV感染患者的治疗结果。患者分别采用SCH503034100毫克每天两次...
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Angiotensinmetabolisminrenalproximaltubules,urine,andserumofsheep:evidenceforACE2-dependentprocessingofangiotensinII
...ationofproximaltubules.TheseprocedureswereapprovedbytheWakeForestUniversitySchoolofMedicineIACUCforanimalcare.Proximaltubulepreparation.ProximaltubuleswerepreparedfromamodifiedmethodofVinayetal.(38).Kidneyoutercortexwasmincedintofinepiecesandincubatedwithcollagenase(1mg/ml,CLS1,Worthington)at37°...
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Voltage-DependentBlockofN-Methyl-D-aspartateReceptorsbyDopamineD1ReceptorLigands
...ngtoNMDAreceptors.Furthermore,usingD1receptorknockoutmice,weverifiedthatthischannel-blockingeffectwasindependentofD1receptors.Finally,wedemonstratedthatD1ligandsfunctionallyinteractwithMg2+blockthroughmultiplesites,implyingapossibleroleofthedirectchannelblockunderphysiologicalconditions.Ourresultssu...
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RenaldopamineD1receptordysfunctionisacquiredandnotinheritedinobeseZuckerrats
...2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepin-7-olhydrochloride([3H]SCH-23390hydrochloride),aD1-receptorantagonist,and35S-labeledguanosine5‘-(-thio)triphosphate([35S]GTPS)werepurchasedfromNewEnglandNuclearLifeSciences.AntibodieswerepurchasedfromAlphaDiagnosticandCalbiochem-Novabiochem.Allot...
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第二节 组织细胞增生症X
... 组织细胞增生症X（histiocytosisX）包括Letterer-Siwe病，Hand-Schüller-Christian病和骨的嗜酸性肉芽肿（eosinophilicgranuloma）3种疾病。这三种疾病的临床表现和病理变化不同。Letterer-Siwe病为急性弥漫性，发展快，常表现为恶性过程；Hand-S...
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过敏性紫癜的心肌损害
...过敏性；血管炎；心肌损害Myocardialinjuryinchildrenwithhench-SchnleinpurpuraCHENXiong.DepartmentofPediatrics,BeihaiMaternalandChildHealthCareHospital,Beihai536000,China［Abstract］ObjectiveToinvestigatetheincidenceandclinicalcharacteristicsofHench-Schnleinpurpurawithmyocardi...
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MolecularInteractionsofCCR5withMajorClassesofSmall-MoleculeAnti-HIVCCR5Antagonists
...alpropertieshavealsobeenreportedbyotherpharmaceuticalcompanies.TheseincludeSCH-C(SCH-351125),vicriviroc(VVC,SCH-D,SCH-417690),aplaviroc(APL,AK602,GW873140),andmaraviroc(MVC,UK-427,857).SCH-Cisanoximino-piperidino-piperidineamide(Palanietal.,2002)thatshowedpotentantiviralactivityinvivo.However,itscli...
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DetectionandQuantificationofHumanImmunodeficiencyVirusType1p24AntigeninDriedWholeBloodandPlasmaonFilterPaperStoredunderVariousCondition
...ctionintoEDTA-containingVacutainertubeswerespottedontofilterpaper(903Paper;SchleicherSchuell,Inc.,Keene,NH).Afterdrying,thespecimenswereplacedincommonplasticZiplocbags.Aliquotsofeachspecimenwerestoredat–80°C,andtotesttheeffectsofvariousambienttemperaturesthatcouldoccurduringspecimentransport,at37...
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过敏性紫癜患儿抗血管内皮细胞抗体检测的意义
...ignificanceofanti-endothelialcellsantibodies（AECA）inpatientswithhenoch-schonleinpurpuraTANGXue-qing,SUNWen-ping,ZOUZi-ying.etal.DepartmentofPediatrics,ChengduPLACentralHospital,Chengdu610083,China［Abstract］ObjectiveTostudytherolesofanti-endothelialcellsantibodies（AECA）inthepathogenesisan...
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OxidativeStressPromotesEndothelialCellApoptosisandLossofMicrovesselsintheSpontaneouslyHypertensiveRats
...ing,UniversityofCalifornia,SanDiego,LaJolla,Calif.CorrespondencetoDrGeertW.Schmid-Sch?nbein,DepartmentofBioengineering,WhitakerInstituteofBiomedicalEngineering,UniversityofCalifornia,SanDiego,9500GilmanDrive,LaJolla,CA92093-0412.E-mailgwss@bioeng.ucsd.eduAbstractObjective—Endothelialcellapoptosisc...
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ReducedrenaldopamineD1receptorfunctioninstreptozotocin-induceddiabeticrats
...nishedindiabeticrats.D1receptornumbersandproteinabundanceasdeterminedby[3H]SCH-23390ligandbindingandWesternblotanalysisweremarkedlyreducedindiabeticratscomparedwithcontrolrats.Moreover,SKF-38393failedtostimulateGTPSbindinginproximaltubularmembranesfromdiabeticratscomparedwithcontrolrats.Weconcludeth...
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UseoftheGenotypeMTBDRAssayforRapidDetectionofRifampinandIsoniazidResistanceinMycobacteriumtuberculosisComplexIsolates
ForschungszentrumBorstel,NationalReferenceCenterforMycobacteria,D-23845Borstel,GermanyDepartmentofMicrobiology,LimbachLaboratory,D-69126Heidelberg,GermanyABSTRACTAcommerciallyavailableDNAstripassay(GenotypeMTBDR;HainLifescience,Nehren,Germany)wasevaluatedforitsabilitytodetectmutationsconferringresis...