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脑康散对东莨菪碱造模小鼠学习记忆障碍改善的实验研究
...要】通过跳台法及免疫组化法，观测脑康散对东莨菪碱(Scop)造模小鼠学习记忆功能及5-HT阳性细胞数的影响。在实验中与造模组相比，预防组小鼠触电潜伏期明显延长(P＜0.01)，5min错误次数明显减少；5-HT阳性细胞数显著增多(P＜0.0...
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针刺加当归芍药散治疗阿尔茨海默症的机制研究
....MethodsThreekindsofmodelratswithdysmnesiawhichweremadebyinjectedintodrugofscopolamineorNaNo2intheabdomenseparatelyandinjuredatthehippocampalCA1inbrianbydrugofquinolinicacidtreatedbysingleacupunctureoracupuncturecombinedwithDGSYSofTCMorDGSYSofTCMwereobservedseparately，tocomparedifferentinfluenceon...
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马唐和止血马唐
马唐和止血马唐 学名马唐Digitariasanguinalis(L．)Scop．止血马唐D.ischaemum(Schrebb．)Schreb．属禾本科一年生草本植物。别名抓根草、鸡爪草。广布全国各地。是旱秋作物、果园、苗圃的主要杂草。形态特征秆基开始倾斜，着地后节处...
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HistoneDeacetylaseInhibitorReducesMonocyteAdhesiontoEndotheliumThroughtheSuppressionofVascularCellAdhesionMolecule-1Expression
...-flankingsequenceandthedownstreamsecondintron)coupledtoEGFP.IntravitalMicroscopyStudiesMicewerepretreatedwithanintraperitonealinjectionofTSA(5mg/kg)orDMSOtwice,25hoursand1hourbeforeintraperitoneallipopolysaccharide(LPS)(0.35mg/kg)ormouserecombinantTNF-(20µg/kg)injection.TheselecteddosesofLPSan...
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无痛苦胃镜下APC治疗疣状胃炎的临床价值
...子体凝固术；疣状胃炎ClinicalvalueonAPCtreatmentwithnopaingastroscopyforverrucousgastritisXIEHua.EndoscopyCenter,ThePeople’sHospitalofJiangjin,Chongqing402260,China［Abstract］ObjectiveToinvestigateargonplasmacoagulation（APC）withoutpainingastroscopeinthetreatmentofverrucousgastr...
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曙光生物基因序列研究解决方案
...B、转录因子和结合位点库TRANSFAC、蛋白质结构家族分类库SCOP等等。 3.基因序列比对和数据库搜索 比较是科学研究中最常见的方法，通过将研究对象相互比较来寻找对象可能具备的特性。在生物信息学研究中，比对是最常...
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腹腔镜下胃癌根治性切除研究进展
...国普通外科杂志,2006,9：641-642.4KitanoS,IsoY,MoriyamaM,etal.Laparoscopy-assistedbill-rothIgastrectomy.SurgLaparoscEndosc,1994,2:146-148.5GohP,TekantY,KumCK,etal.Totallyintra-abdominallaparo-scopicBillrothⅡgastrectomy.SurgEndosc,1992,6(3):160.6KitanoS,ShiraishiN.Minimallyinvasivesurgeryforg...
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当前生物信息学的重要研究任务
...的启发。著名的二级数据库象：蛋白质结构分类数据库（SCoP），受体数据库，克隆载体数据库等……。 1.2以因特网（Internet）为基础的基因组信息学传输网络 用户与数据库间迅速、有效地传递信息是基因组信息的收集、...
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Asp92AsnPolymorphismintheMyeloidIgAFcReceptorIsAssociatedWithMyocardialInfarctioninTwoDisparatePopulations
...0.007).Intheplaceboarmoftheall-maleWestofScotlandCoronaryPreventionStudy(WOSCOPS),carriershadanadjustedoddsratioforincidentcoronaryheartdisease(CHD)of1.46(90%CI1.05to2.03);forpravastatincomparedwithplacebotreatment,theadjustedoddsratioswere0.55(95%CI0.32to0.93)incarriersand0.65(95%CI0.51to0.83)innon...
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Activatedextracellularsignal-regulatedkinasesarenecessaryandsufficienttoinitiatetubulogenesisinrenaltubularMDCKstrainIcellcysts
...ontaneouslyinitiatetheprimarystepsintubulogenesis.Analysisoftime-lapsemicroscopyofbothMDCKstrainIandstrainIIcellcystsduringtheinitialstagesoftubulogenesisdemonstratesahighlydynamicprocesswithcellularextensionsandretractionsoccurringrapidlyandcontinuously.MDCKstrainIcellcystscanspontaneouslyinitiatet...
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Whycottonwoolspotsshouldnotberegardedasretinalnervefibrelayerinfarcts
...refirstobservedinhypertensiveretinopathysoonaftertheinventionoftheophthalmoscope.Theyarepotentialcomponentsofthefunduspictureinawidevarietyofsystemicdiseases,ortheymayaccompanysignsofretinalvascularocclusion.Assuch,CWSsoftencoexistwithotherretinopathicfeatureslikehaemorrhages,lipidexudatesoroedema,o...
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cPLA2-interactingprotein,PLIP,causesapoptosisanddecreasesG1phaseinmesangialcells
...dieswereobtainedfromSantaCruzBiotechnologyandusedatadilutionof1:5,000.Microscopicanalysisofapoptosis.Mesangialcellswereplatedinsix-wellplates,infectedwithAd-LacorAd-PLIPasdescribed,andgrowninRPMI/10%FCS.After36days,cellsweregentlytrypsinizedandcellsplussupernatantwerecentrifugedandresuspendedin30μl...
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HypoxiaInhibitsPaclitaxel-InducedApoptosisthroughAdenosine-MediatedPhosphorylationofBadinGlioblastomaCells
...dwith0.4%trypanbluefor5minatroomtemperaturebeforebeingexaminedunderthemicroscope.Thenumberofviablecellswasdeterminedbytrypanblueexclusion.Thedeadcellsthatstainedbluewerescoredpositiveandcountedagainstthetotalnumberofcellstodeterminethepercentageofcelldeath.ATPLiteAssay.TheintracellularATPconcentrati...
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NeuropeptideFF(NPFF)AnalogsFunctionallyAntagonizeOpioidActivitiesinNPFF2Receptor-TransfectedSH-SY5YNeuroblastomaCells
...epresentresults.ForeachPetridish,theresponseswererecordedintwodistinctmicroscopefields.Eachexperimentwasperformedonatleasttwodifferentcultures.Thereduction(%)ofthedepolarization-induced[Ca2+]itransientbyDAMGOwascalculatedas100x[(F/F0)DAMGOeC(F/F0)C]/(F/F0)C,inwhich(F/F0)C=((F/F0)maxeC1)Cand(F/F0)DAM...
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MutagenesiswithinHelix6oftheHuman1-AdrenergicReceptorIdentifiesLysine324asaResidueInvolvedinImpartingtheHigh-AffinityBindingStateofAgoni
...mager(PerkinElmerLifeandAnalyticalSciences).AcidStripConfocalRecyclingMicroscopyProtocol.HEK-293cellsexpressingtheFLAG-taggedWT1-ARorFLAG-taggedK324Amutantweregrownonpoly-L-lysine-coatedglasscoverslipsandserum-starvedat37°Cfor1hinDMEMsupplementedwith25mMHEPES,pH7.4.Thereceptorswerelabeledwith5&m...