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Rapid,High-Throughput,Multiplex,Real-TimePCRforIdentificationofMutationsinthecyp51AGeneofAspergillusfumigatusThatConferResistancetoItracon
...ancetoitraconazoleinA.fumigatusiscloselylinkedtoaminoacidsubstitutionsinCyp51AthatreplaceGly54.Inanefforttodevelopanewclassofmoleculardiagnosticassaythatcanrapidlyassessdrugresistance,amultiplexedassaywasestablished.Thisassayusesmolecularbeaconscorrespondingtothewild-typecyp51Ageneandsevenmutantalle...
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DetectionofAspergillusfumigatusandaMutationThatConfersReducedSusceptibilitytoItraconazoleandPosaconazolebyReal-TimePCRandPyrosequencing
...ethodwasdevelopedtodetectAspergillusfumigatusinwholebloodbyamplifyingthecyp51Ageneandsequencingthecodonforglycine54.Mutationsinthiscodoncanresultinaminoacidsubstitutionsthatconferreducedsusceptibilitytoitraconazoleandposaconazole.TEXTInvasiveaspergillosisisamajorcauseofmorbidityandmortalityinimmunoc...
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A46-AminoAcidSegmentinPhosphodiesterase-5GAF-BDomainProvidesforHighVardenafilPotencyoverSildenafilandTadalafilandIsInvolvedinPhosphodiest
...finityforcertaininhibitors,fourN-terminaltruncationmutantsweregenerated:PDE51-321containedGAF-Bdomain,Cdomain,andthesequencebetweenGAF-Aand-B;PDE51-419containedGAF-BandCdomain;PDE51-465containedtheCdomainandtheC-terminalportionofGAF-B;andPDE51-534containedonlyCdomain.TruncatedproteinswithacompleteGA...
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ActivatedIGF-1Rinhibitshyperglycemia-inducedDNAdamageandpromotesDNArepairbyhomologousrecombination
...ctDNArepairbyNHEJ.IGF-1RenhancementofHRRcorrelatedwiththetranslocationofRad51tofociofDNAdamage.InhibitionofRad51expressionbyshortinterferingRNAexperimentsmarkedlydecreasedpercentageofMMCpositiveforRad51nuclearfociandincreasedhyperglycemicDNAdamage.WeconcludethattheactivatedIGF-1Rrescuesmesangialcell...
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TheRoleofThr139intheHumanImmunodeficiencyVirusType1ReverseTranscriptaseSensitivityto(+)-CalanolideA
...ET.Whereasthisloopisexposedtothesolventinthep66subunit,theequivalentloopinp51issnuglylodgedintoacleftonthesurfaceofthep66subunit(Kohlstaedtetal.,1992).Infact,thisloop,whichisclosetoboththeputativeentrancetotheNNRTI-bindingpocketandtheactivesite(Fig.1),isessentialforthecatalyticfunctionofthep66subuni...
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TransmissionandaccumulationofCTLescapevariantsdrivenegativeassociationsbetweenHIVpolymorphismsandHLA
...ressureexertedonHIVbyCTLs,wehavefocusedonthreealleles,HLA-B*57,B*5801,andB*51,whichareassociatedwitheffectivesuppressionofviremia(10–15)andthereforelikelytoimposestrongselectionpressureonthevirus.HLA-B*57andB*5801arecloselyrelated,targetingmanyofthesameepitopes(16)andselectingforthesameescapemutat...
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SelectiveactivationofSTAT5unveilsitsroleinstemcellself-renewalinnormalandleukemichematopoiesis
...einasimilarfashion(18,19,andunpublisheddata).AmongSTAT5mutants,however,STAT51*6andSTAT51*7mutantscouldmediatehigh-leveltranscriptionalactivationofaSTAT5targetgene,whereasSTAT5#2waslesseffective(Fig.2B).Westernblottingassessmentshowedcomparableexpressionofeachmutantintransducedcells(Fig.2B).Selective...
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AssessingtheSerodiagnosticPotentialof35MycobacteriumtuberculosisProteinsandIdentificationofFourNovelSerologicalAntigens
...two-stepselectionprocess,foursuperiorseroantigens,TB9.7,TB15.3,TB16.3,andTB51,wereidentified,noneofwhichhasbeendescribedbefore.Thefournovelantigensweretestedwithpanelsofserafromsmear-positiveandsmear-negativeTBpatientsfromareasbothwhereTBisendemicandwhereTBisnotendemic,withrecognitionfrequenciesrang...
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MolecularDetectionofCellulosimicrobiumcellulansastheEtiologicalAgentofaChronicTongueUlcerinaHumanImmunodeficiencyVirus-PositivePatient
...yrichinCYPenzymes(4,9,13).Moreover,anorthologofthespecificP450CYPenzyme(CYP51)hasbeenidentifiedinM.tuberculosis,Mycobacteriumsmegmatis(8,18),andRhodococcusspp.,andithasbeenpostulatedthataCYP51-likeP450enzymewaspresentinanancestralactinomycete,fromwhichitmayhavebeeninheritedbythemembersofthisorder(10...
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Five-YearFollow-UpStudyofMother-to-ChildTransmissionofHelicobacterpyloriInfectionDetectedbyaRandomAmplifiedPolymorphicDNAFingerprintingMetho
...NAanalysesusingarandomamplifiedpolymorphicDNAfingerprintingmethod.Noneofthe51childrenacquiredH.pyloriinfectionduringthefirstyearoflife.Ofthe44childrenenrolledina5-yearfollow-upstudy,five(11%)acquiredH.pyloriinfection.Theyacquiredtheinfectionattheageof1year2months,1year3months,1year6months,1year8mont...
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PhylogeneticAnalysisofthep24-p7RegionoftheHumanImmunodeficiencyVirusType1gagGeneToDetermineSubtypeDistributionamongFemaleSexWorkersinC
...ion,hasbeenreportedtobepredominantinIndia.Wesequencedthep24-p7gagregionfrom51HIV-1seropositivefemalesexworkersinCalcutta,India,formore-detailedmolecularcharacterization.SubtypeCwasfoundtobeprevalent,althoughnostrongmonophyleticclusterwasobserved.TEXTSincethefirstreportofhumanimmunodeficiencyvirustyp...
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CentralEuropeanDobravaHantavirusIsolatefromaStripedFieldMouse(Apodemusagrarius)
...cesfromA.agrariusledtothedefinitionofasecondgeneticDOBVclade,DOBV-Aa(15,50,51).DozensofHFRSpatientswithDOBVinfectionhavebeendiagnosedbytypingtheirneutralizingantibodies,andtheclinicalparametersofthesepatientshavebeenwellcharacterized;incontrasttothesituationinSouth-EastEurope,HFRScasescausedbyDOBVin...
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UltrasoundStimulatesCyclooxygenase-2ExpressionandIncreasesBoneFormationthroughIntegrin,FocalAdhesionKinase,Phosphatidylinositol3-Kinase,andAkt
...nandmRNAlevelsofcyclooxygenase-2(COX-2).Atthemechanisticlevel,anti-integrin51andv3antibodiesorrhodostomin,asnakevenomdisintegrin,attenuatedtheUS-inducedCOX-2expression.Phosphatidylinositol3-kinase(PI3K)inhibitors2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-onehydrochloride(LY294002)andwortmanninals...
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MolecularCharacterizationofFoot-and-MouthDiseaseVirusTypeCofIndianOrigin
...usesrevealedthattheIndianvirusesformadistinctgenotype.ThevaccinestrainCIND/51/79belongstothisgenotypeandmaybeaprototypestrainofthisgenotype.TEXTFoot-and-mouthdisease(FMD)isanacutecontagiousviraldiseaseofcloven-footedlivestock,andthevirusexistsassevenserotypes:A,O,C,Asia1,SATI,SATII,andSATIII.Thetype...
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MolecularDetectionandQuantitativeAnalysisoftheEntireSpectrumofHumanAdenovirusesbyaTwo-ReactionReal-TimePCRAssay
...amountimportance.Apparently,anyofthesixAdVspecies(AtoF),currentlycomprising51differentserotypes,canplayaclinicallyimportantroleinpatientswithimpairedimmuneresponse.Itisimperativethereforethatdiagnosticassayscovertheentirespectrumoftheseviruses.Wehavesequencedpresumablyconservedregionsoftheadenoviral...
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乳腺叶状囊肉瘤（叶状肿瘤）的组织发生与组织分化
...瘤的恶性间叶成分的组织分化呈多样性。免疫组化观察的51例叶状囊肉瘤、8例巨大纤维瘤、5例纤维腺瘤中Vim标记均阳性，51例叶状囊肉瘤中、Act、S-100、lys、Des分别有28、19、3、1例阳性，8例的巨大纤维腺瘤中仅Act阳性1例。结论...
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脊柱动力平衡KKT技术治疗特发性脊柱侧弯疗效观察
...对10例特发性脊柱侧弯的患者用KKT技术进行治疗，疗程8～51.3周，每周2次，平均19.2周。观察治疗前后患者站立位X线片主弯段Cobb’s角的变化。［结果］10例中6例Cobb’s角有改善，改善度为1°～13°，平均6.17°，4例Cobb’s角治疗前后...
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UrinaryD-Arabinitol/L-ArabinitolLevelsinInfantsUndergoingLong-TermAntibioticTherapy
...)wasdeterminedbygaschromatographywithanelectroncapturedetectorinsamplesfrom51infantsundergoinglong-termantibiotictherapy.Although47ofthesechildrenhadhigherD-/L-ARAratiosthanhealthycontrols(P0.0003),theirvaluesnonethelessremainedwithinupper-normallimits(D-/L-ARAratioof3.6).Fourchildrenwithsuspectedin...
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Theagingprocessasamodifierofmetabolism
...minB-12arenotdifferentforpersonsintheoldestagecategory(70y)thanforthoseaged51–70y.Becausethisisaquicklyadvancingfield,itwillbeimportanttocloselyfollownewresearchonnutrientrequirementsandagingoverthenextseveralyears.KeyWords:Aging•elderly•nutrientrequirements•metabolism•dieta...
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大剂量维生素C与消心痛联合治疗心绞痛型冠心病51例临床分析
...的使用大剂量维生素C与消心痛联合治疗心绞痛型冠心病51例，改善冠脉供血，提高生活质量，减少病死率。方法大剂量维生素C静脉滴注与消心痛口服。结果治疗心绞痛型冠心病有效率达96%。结论大剂量维生素C静脉滴注与消心痛...
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InteractionStudiesofMultipleBindingSitesonM4MuscarinicAcetylcholineReceptors
...7--hydroxy-17--ethynyl-5--androstano[3,2-b]pyrimido[1,2-a]benzimidazole(WIN51,708),exhibiteddifferentdegreesofpositive,negative,orclose-to-neutralcooperativitywiththeorthostericsiteonM1orM4mAChRs,dependingonthechemicalnatureoftheorthostericradioligandthatwasused[[3H]N-methylscopolamine([3H]NMS)versu...
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Fatandenergyneedsofchildrenindevelopingcountries
...ternationalNutritionGroup,LondonSchoolofHygieneandTropicalMedicine,49–51BedfordSquare,LondonWCIB3DP,UnitedKingdom.E-mail:andrew.prentice{at}lshtm.ac.uk.ABSTRACTThefatrequirementsofchildrencanbejudgedaccordingto4criteria:1)thepossibleobligateneedsoffatasametabolicfuel,2)theprovisionofasufficient...
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多巴酚丁胺对心肌桥-壁冠状动脉血流动力学的作用
...结果多巴酚丁胺使壁冠状动脉受压程度由用药前的平均(51.7±21.4)%增加至(90.0±12.7)%，P＜0.01；壁冠状动脉近段和远段的hAPV分别由(19.83±5.84)cm/s和(20.75±4.91)cm/s增加至(31.52±10.93)cm/s和(30.46±9.01)cm/s；壁冠状动脉近段和远段的CFR分别由(...
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TheAminoAcidAsn136inHIV-1ReverseTranscriptase(RT)MaintainsEfficientAssociationofBothRTSubunitsandEnablestheRationalDesignofNovelRTInhi
...tofthemutationsatposition136occurredwhenthemutatedaminoacidwaspresentinthep51subunitbutnotinthep66subunitofthep51/p66RTheterodimer.Allmutantenzymescouldbeinhibitedbysecond-generationNNRTIssuchasefavirenz.Theywerealsomarkedlymoresensitivetotheinactivating(denaturating)effectofureathanwild-typeRT,andt...
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NIHSSTrainingandCertificationUsingaNewDigitalVideoDiskIsReliable
...resufficientpowerforassessingoverallNIHSSscorereliability,eachcenterwassent51DVDswithscoringsheets.ThepoweranalysisisbasedontheintraclasscorrelationcoefficientfortheoverallNIHSSscoreandwasperformedusingthesoftwarePASS.Asamplesizeof18subjectswith51observationspersubjectachieves80%powertodetectanintra...
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3种摄片方法预测青少年特发性脊柱侧凸矫形结果的对比研究
...和19°；僵硬组与柔软组重力悬吊牵引位片Cobbs角分别是51°和22°，仰卧侧屈位片Cobbs角分别是52°和22°，支点弯曲位片Cobbs角分别是48°和18°，术后站立位片Cobbs角分别是45°和17°；前路手术组与后路手术组重力悬吊牵引位...
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BRCA1ContributestoCellCycleArrestandChemoresistanceinResponsetotheAnticancerAgentIrofulven
...omosomebreaksandradialsandtheactivationandfociformationof-H2AX,BRCA1,andRAD51.WealsoprovidedevidencethatirofulveninducesthegenerationofDNAdouble-strandbreaks.ByusingBRCA1-deficientor-proficientcells,wedemonstratedthatinresponsetoirofulven,BRCA1contributestothecontrolofSandG2/Mcellcyclearrestandiscri...
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CerebralVeinandDuralSinusThrombosisinElderlyPatients
...isolatedintracranialhypertensionsyndromewaslessfrequentinelderlypatients(4/51versus139/573,P=0.008),whereasdepressedconsciousness(17versus97,P=0.005),andmentalstatuschanges(22versus115,P=0.001)weremorefrequentintheelderly.Theprognosisofelderlypatientswasconsiderablyworsethanthatofyoungerpatients,aso...
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精神分裂症患者家属情绪障碍的心理干预
...43名，38.98+8.45)。干预组（男75名，49.5±7.43；女35名，51.5±8.10），t值分别为8.9312、8.4699，P均＜0.01；对照组（男76名，49.6±7.36；女34名，51.3±8.117），t值分别为9.0856、8.2221，P均＜0.01。 2.2干预组和对照组干预...
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IschemicandHemorrhagicStrokesinDakar,Senegal
...terminedorigin.Mortalitywithin1monthwas38%.Forhemorrhagicstrokes,meanagewas51yearsand1monthmortalitywas56%.Conclusion—Hypertensionisthemainriskfactorforbothischemicandhemorrhagicstrokesinthishospital-basedstudy.KeyWords:Africa,WesternriskfactorsstrokeIntroductionAlthoughstrokeisnowamajorpublicheal...