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新型HLA衍生肽对大鼠脾细胞免疫功能的抑制作用
...庆）为了观察一种新型组织相容性白细胞抗原(HLA)衍生肽(RDP1258)的免疫抑制作用并探讨其机制。方法人工合成一种HLA-RDP1258，用3H胸腺嘧啶(3H-TdR)掺入法观察其在体外对大鼠脾细胞增殖反应的影响,以酶化学法观察其对脾细胞血红素...
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核酸及其相关数据库资源
...noGeneTicsdb[MirroratEBI ISIS-IntronSequenceandInformationSystem RDP-RibosomaldbProject gRNAsdb-GuideRNAdb PLACE-Plantcis-actingregulatoryDNAelementsdb PlantCARE-Plantcis-actingregulatoryDNAelementsdb sRNAdb-SmallRNAdb ssurRNA-Smallribosomalsubunitdb lsurRNA-L...
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髌骨不稳定的诊治现状
...］。Nam应用小切口行内侧支持带折叠结合关节镜下LRR治疗RDP23膝，随访4.4年，结果6膝优、15膝良、2膝可，平均Kujala评分88.2［14］。 (3)Campbell术：用关节囊组织条向内牵拉股四头肌腱。这种方法适用于年龄小、脱位轻、骨发育异...
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推荐：二代测序数据常用的4种pipeline浅谈
...率就会大一些。对于大量数据的处理，有一定的帮助。4.RDPpipeline(http://pyro.cme.msu.edu/)由密西根州立大学的美国科学院院士JamesM.Tiedje以及JameR.Cole领衔的团队开发的，RDP的特征是在线的，对于国外用户，要首先将自己的数据传输到...
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MolecularIdentificationofCapnocytophagaspp.via16SrRNAPCR-RestrictionFragmentLengthPolymorphismAnalysis
...ycomparisonwiththe16SrRNAgenedatabaseslocatedattheRibosomalDatabaseProject(RDP-II;MichiganStateUniversity,EastLansing)(22);theywerealsoanalyzedwiththebasiclocalalignmentsearchtool(BLAST;version2.2.1)(1,2,28).Stringentcriteria(tall,distinctpeaks,minimalnumberof“Ns“(non-calledbases),andverylowback...
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ClinicalUseof16SrRNAGeneSequencingToIdentifyMycoplasmafelisandM.gateaeAssociatedwithFelineUlcerativeKeratitis
...siswasperformedbyonlineanalysisattheRibosomalDatabasePro-jectIIsite(http://rdp8.cme.msu.edu/html/index.html)andtheMicroSeqDatabaseLibrary(AppliedBiosystems,FosterCity,CA)aspreviouslydescribed(15,23).Theprocessofamplifyingandsequencingthe16SrRNAgeneofmostisolatesrequired1to2days(23).Thesequencesofthe...
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Quantitationofinvivohumanfolatemetabolism
...atory,Livermore,CA(BABandJSV);andIntegrativeBioInformaticsInc,Rockville,MD(RDP)2SupportedbyNIHDK45939andResearchResourceGrantRR-13461fromtheNIH.ThestudywasperformedinpartundertheauspicesoftheUSDepartmentofEnergybytheUniversityofCaliforniaLawrenceLivermoreNationalLaboratoryundercontractno.W-7405-Eng-...
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CultivableOralMicrobiotaofDomesticDogs
...pocket.Nineplaquesamplesweretakenfromthebuccalsurfacesoftheuppersecondorthirdpremolarsoftheninedogs.Inaddition,asinglesupragingivalplaquesamplewastakenfromanuppersecondpremolar,andadistalandmesialpooledsamplewastakenfromtheperiodontalpocketonanuppersecondincisor,withbothoftheseextrasamplesbeingtaken...
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Porphyromonassomeraesp.nov.,aPathogenIsolatedfromHumansandDistinctfromPorphyromonaslevii
...oftheconsensussequenceswithGenBanksequencesbyusingRibosomalDatabaseProject(RDP-II;MichiganStateUniversity,EastLansing)(15)andBasicLocalAlignmentSearchTool(BLAST)software(2),andthepercentsimilaritytoothersequenceswasdetermined.CloselyrelatedsequenceswereretrievedfromGenBankandwerealignedwiththenewlyd...
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Evaluationof16SrRNASequencingandReevaluationofaShortBiochemicalSchemeforIdentificationofClinicallySignificantBacteroidesSpecies
...heconsensussequenceswithGenBanksequencesbyusingtheRibosomalDatabaseProject(RDP-II)(MichiganStateUniversity,EastLansing,Mich.)(15)andtheBasicLocalAlignmentSearchTool(BLAST)(1).AnalysisofBacteroidesclinicalisolateswasperformedbycomparingthesequencesagainstthesequencesoftypestrainsdeterminedinthisstudy...
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MolecularAnalysisofMicrobialDiversityinAdvancedCaries
...peratureof4°C.ThetriplicatePCRsampleswerecombinedandpurifiedbyusingtheWizardPCRPrepsDNApurificationsystem(Promega,Sydney,NewSouthWales,Australia)beforebeingclonedintolinearizedplasmidsbyusingthepGEM-TEasyvectorsystem(Promega)accordingtothemanufacturer‘sinstructions.Ligatedplasmidsweretransformedi...
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TNF-308ModifiestheEffectofSecond-HandSmokeonRespiratoryIllness–relatedSchoolAbsences
...erExpressapplications-basedprimerdesignsoftware(AppliedBiosystems).TheforwardprimerforhumanTNFwas5‘-GAAATGGAGGCAATAGGTTTTGA-3‘,andthereverseprimerwas5‘-GTAGGACCCTGGAGGCTGAAC-3‘.Theminorgroove-bindingprobefortheGallelewas5‘-CCGTCCCCATGCC-3‘,andtheminorgroove-bindingprobefortheAallelewas5...
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DefiningtheNormalBacterialFloraoftheOralCavity
...analyzed.Sitesincludedtonguedorsum,lateralsidesoftongue,buccalepithelium,hardpalate,softpalate,supragingivalplaqueoftoothsurfaces,subgingivalplaque,maxillaryanteriorvestibule,andtonsils.16SrRNAgenesfromsampleDNAwereamplified,cloned,andtransformedintoEscherichiacoli.Sequencesof16SrRNAgeneswereusedtod...
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MultilocusSequenceTypingofSwedishInvasiveGroupBStreptococcusIsolatesIndicatesaNeonatallyAssociatedGeneticLineageandCapsuleSwitching
...sistyping,pulsed-fieldgelelectrophoresis,restrictiondigestionpatterntyping(RDP),andrestrictionfragmentlengthpatternanalysis(25-28,30).DiverselineagesamongserotypeIIIisolateshavebeendistinguished,andlineageshavebeensuggestedtodifferintheirvirulencepotentialsandtissuetropisms.However,infewofthesestudi...
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IdentificationofCandidatePeriodontalPathogensandBeneficialSpeciesbyQuantitative16SClonalAnalysis
...himerasbyusingtheChimeraCheckprogramoftheRibosomalDatabaseProjectII(http://rdp.cme.msu.edu/html/).Twenty-threecloneswereidentifiedaschimericsequencesandexcludedfromfurtheranalysis.Sequenceswerealigned,andasimilaritymatrixwasconstructedfromthealignmentsbythemethodofJukesandCantor.Phylogenetictreeswer...
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Rai1duplicationcausesphysicalandbehavioralphenotypesinamousemodelofdup(17)(p11.2p11.2)
...tudieswereperformedinmalemice,sincegenderspecificitywaspreviouslyobservedforDp(11)17/+mice(22).WewereabletorescuemanyofthebehavioralphenotypesobservedinthemaleanimalswithsegmentalaneuploidiesbynormalizingthegenecopynumberinDp(11)17/Df(11)17mice(Figures2and3).Theverticalactivityandcenter/totaldistanc...
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StimulationofNa+transportbyAVPisindependentofPKAphosphorylationoftheNa-K-ATPaseincollectingductprincipalcells
...tsgeneratedbyPCR.Serine943wassubstitutedeitherbyalanine(S943A)usingtheforwardprimerCCAGGAGGAATGCAGTCTTCCAGCAGGandthereverseprimerCCTGCTGGAAGACTGCATTCCTCCTGGorbyasparticacid(S943D)usingtheforwardprimerCCAGGAGGAATGACGTCTTCCAGCAGGandthereverseprimerCCTGCTGGAAGACGTCATTCCTCCTGG.Thefull-lengthwild-typeand...
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RapidIdentificationofBacteriafromPositiveBloodCulturesbyTerminalRestrictionFragmentLengthPolymorphismProfileAnalysisofthe16SrRNAGene
...sbyusingtheT-RFLPanalysisprogram(TAPT-RFLP)oftheRibosomalDatabaseProjectII(RDP-II)(19).Asindicated,primerswerelabeledatthe5‘endwiththedyes6-carboxyfluorescein(6FAM),4,7,2‘,4‘,5‘,7‘-hexachloro-6-carboxyfluorescein(HEX),orN,N,N‘,N‘-tetramethyl-6-carboxyrhodamine(TAMRA).Allprimerswerepurc...
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UseofPhylogeneticandPhenotypicAnalysesToIdentifyNonhemolyticStreptococciIsolatedfromBacteremicPatients
...especies.ThisisanoptionatthedatabaseoftheRibosomalDatabaseProjectII(http://rdp.cme.msu.edu/index.jsp).Ourstudydemonstratesthatunequivocalidentificationofclinicalisolatesofnonhemolyticstreptococcicanbeachievedbyphylogeneticanalysisofconcatenatedsequencesoffourhousekeepinggenes,ddl,gdh,rpoB,andsodA,am...
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Thestorysofar:molecularregulationofthehemeoxygenase-1geneinrenalinjury
...iMC,ChristophF,WooJ,IyerS,BrouardS,HeslanJM,PignonP,SoulillouJP,andBuelowR.RDP1258,anewrationallydesignedimmunosuppressivepeptide,prolongsallograftsurvivalinrats:analysisofitsmechanismofaction.MolMed5:820-832,1999.DaiC,YangJ,andLiuY.Transforminggrowthfactor-1potentiatesrenaltubularepithelialcelldeat...
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CoordinatedoncogenictransformationandinhibitionofhostimmuneresponsesbythePAX3-FKHRfusiononcoprotein
...efor10mintoallowvisualizationoftheantibody.IL-10andDCmaturationassays.5x105RDP3F-ERcellswereplatedinavolumeof2ml,and1μM4HTwasaddedfor48htoinducePAX3-FKHRfunction.ForDCmaturationinhibitionexperiments,PBMCswereculturedinRPMI1640supplementedwith2%humanABseruminthepresenceof100ng/mlGM-CSFand10ng/mlIL-4...
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BacterialDiversityinCasesofLungInfectioninCysticFibrosisPatients:16SRibosomalDNA(rDNA)LengthHeterogeneityPCRand16SrDNATerminalRestrictio
...ncbi.nlm.nih.gov/blast/)andbyusingtheRibosomalDatabaseProjectII(http://www.rdp.cme.msu.edu/html/).SequencesobtainedinthepresentstudyarestoredunderaccessionnumbersAJ491776toAJ491780andaccessionnumbersAJ496326toAJ496330.T-RFLPamplificationandprofiling.(i)T-RFLPPCRproductamplification.PCRproductsforT-R...
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FXYDproteins:newregulatorsofNa-K-ATPase
...theNa-K-ATPasehavebeenidentifiedasacauseofrapid-onsetdystonia-Parkinsonism(RDP;DYT12)(25).DysregulationofNa-K-ATPasethroughadefectiveproductionorfunctionofatissue-specificregulatorhasbeencorrelatedwithvariousdisorders,includingcardiovascular,neurological,renal,andmetabolicdiseases,butthedirectlinkbe...
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常用生物软件（windows）全面介绍
...比较查看器GDA：主要用来进行不连续基因数据的统计分析RDP：从一组排队比较(Align)的核酸序列中查找潜在的重组体软件Sequencher：装配DNA小片段为大的连续序列或毗连(序列)群“Contig“软件MehCalc：自动计算DNA序列热力学数据的Exce...