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克隆病可溶性细胞因子受体的表达谱
...类固醇治疗(n=9)的活动性CD(aCD)患者，临床环节起的CD患者(rCD,n=20)，溃疡性结肠炎患者(UC,n=24)及健康人(HS,n=15)。我们也取了CD患者发炎(n=8)、未发炎(n=7)及健康人(n=8)的结肠黏膜活检。肿瘤坏死因子(TNF-)，可溶性TNF受体I(sTNFRI)，可溶...
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VascularEndothelialGrowthFactor-AMediatesUltravioletB-InducedImpairmentofLymphaticVesselFunction
...mation(Figure1,AandB).Double-immunofluorescencestainsforthepanvascularmarkerCD31andforthelymphatic-specifichyaluronanreceptorLYVE-1revealedmoderateenlargementofCD31+/LYVE-1Cbloodvessels(Figure1,CandD),inagreementwithpreviousstudies.5Surprisingly,LYVE-1-positivecutaneouslymphaticvesselsweredramatical...
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医用不接地供电系统的原理及应用
...常规照明灯电源不必通过隔离变压器。（4）采用≤30mA的RCD保护的设备不必通过隔离变压器。（5)增加线路的绝缘等级,辅助以局部等电位接地，等电位干线保证在16mm2，支线在6mm2以上。IT系统的PE线仍是医院内TNS系统的PE线延伸...
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NaturalKillerTCellsinAtherosclerosis
...is.JVascSurg.2002;35:368–375.AslanianAM,ChapmanHA,CharoIF.TransientRoleforCD1d-RestrictedNaturalKillerTCellsintheFormationofAtheroscleroticLesions.ArteriosclerThrombVascBiol.2005:628–632.MillonigG,SchwentnerC,MuellerP,MayerlC,WickG.Thevascular-associatedlymphoidtissue:anewsiteoflocalimmunity.Cur...
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磁极化冷凝离子吸附血液平衡疗法对糖尿病红细胞变形能力的影响
...mellitus（DM）.Wealsoprobedintothechangingsystemofredcelldeformability（RCD）andtherecyclingsystemofbloodbalancetreatment.Methods70patientsandhealthypersonswhowerewiththemagneticquantumbloodbalancetreatmentwereobservedeveryotherday.Everysixtimeswasoneperiodoftreatment.Wemea-suredtheRCD，bloodli...
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半边旗二萜类化合物5F固体分散体的研究（基金项目）
...限公司）。UV-3101PC紫外分光光度计（日本岛津制作所）;RCD-6型药物溶出仪（上海黄海药检仪器厂）;红外光谱仪（Bio-RadFTS175C傅里叶变换红外光谱仪），测试条件:波数范围400～4000，精度4cm-1，扫描次数32，KBr压片;扫描电子显微镜...
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ThestructureofhumanCD23anditsinteractionswithIgEandCD21
...0892.IgEbindstoitseffectorcellsthrougheitherFcRI,thehigh-affinityreceptor,orCD23(FcRII),thelow-affinityreceptor.TheformermediatestheIgEeffectorfunctionswithmastcellsandbasophilsintypeIimmediatehypersensitivity.CD23hasmultiplefunctionsthatarecontrolledbyarangeofdifferentligands.TheseincludeIgE(bothin...
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LowCD4TCellCountsbeforeHIV-1SeroconversionDoNotAffectDiseaseProgressioninEthiopianFactoryWorkers
...ckground.Humanimmunodeficiencyvirustype1(HIV-1)uninfectedEthiopianshavelowerCD4TcellcountsthandootherpopulationsinAfricaandindustrializedcountries.WestudiedwhetherthisuniqueimmunologicalprofileresultsinshortersurvivaltimesinHIV-1infectedEthiopians.Methods.Datafromanopencohortstudyof149HIV-1infectedf...
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Platelet-derivedorsolubleCD154inducesvascularizedallograftrejectionindependentofcell-boundCD154
...acallograftsafterreceivingCD154-expressinghumanplateletsorrecombinantCD154(rCD154)trimers.TreatmentwiththehumanCD154-specificmAb5c8specificallypreventsthisinducedrejection.Solubletrimers,butnotplatelets,inducerejectionwheninfusedtemporallyremotefromthesurgicalprocedure,suggestingthatsurgicallyinduce...
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PPAR-Agonists:ShiftingAttentionfromtheBellytotheHeart?
...larabnormalities.8Inmonocytes/macrophages,TZDsupregulatethescavengerreceptorCD369andinducethecholesteroleffluxpompATP-bindingcassette,subfamilyA,member1(ABCA1),suggestingalteredlipidhandlingbymacrophageswherebyproatherogeniclipoproteinsaretakenupandantiatherogeniclipoproteinsaregenerated.10Finally,a...
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PersistenceofKaposiSarcomaAssociatedHerpesvirus(KSHV)InfectedCellsinKSHV/HIV-1CoinfectedSubjectswithoutKSHV-AssociatedDiseases
...DetectionofKSHVDNAinlymphnodeswasnotcorrelatedwitheitherplasmaHIV-1RNAloadorCD4+lymphocytecount(P.1,forbothcomparisons).KSHVDNAwasnotdetectedinPBMCsorplasmasamplesfromthe7subjectswithKSHVDNAintheirlymphnodes,andnoneofthesesubjectssubsequentlydevelopedKS(medianfollow-upduration,40months;rangeoffollow...
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CigaretteSmokeActivatesHumanMonocytesbyanOxidant-AP-1SignalingPathway:ImplicationsforSteroidResistance
...tiallymorphologicallyunderlightmicroscopy,andthenbyflowcytometricanalysisforCD14expressionandtheirforwardandsidescatterproperties.MonocyteDifferentiationintoMacrophages.Isolatedmonocytes(70%)weredifferentiatedintomacrophagesusinganestablishedprotocol(Rossietal.,1998).Inbrief,monocytes(2x106/ml)werec...
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EpidemiologyandPredictiveFactorsforChemokineReceptorUseinHIV-1Infection
...19.9%).Only4samples(0.7%)showedexclusiveCXCR4use.Inamultivariatemodel,higherCD4cellcount,lowerviralload,andhighernaturalkillercellcountsweresignificantlyassociatedwithCCR5usage.Noassociationswithtreatmentexperience,clade,orpolgenemutationswereobserved.Conclusion.TheprevalenceofCCR5-tropicHIV-1phenot...
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VascularRemodelinginMiceLackingtheCytoplasmicDomainofTissueFactor
...Figure3C)withnocontaminatingendothelialcellsbecausenocellsstainedpositiveforCD31.14ProliferationofSMCFromTFwt/wtandTFct/ctMiceTodeterminewhethertheenhancedmediaformationinTFct/ctmicewasattributabletoanaugmentedproliferationofSMCsweanalyzedthenumberofproliferatingcellsintheneointimaandmediabyimmunohi...
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NeurogenesisinRatsAfterFocalCerebralIschemiaisEnhancedbyIndomethacin
...danimals.IndomethacinReducesInflammatoryCellActivationSectionswerestainedforCD11b,ageneralmarkerforinflammatorycells.Therewasnosignificantdifferenceinthedensityofmicrogliabetweentreatedandcontrolanimalsat7,14,or28days(Figure6A).WealsoexaminedtissuesforED-1+cells,amarkerforactivatedmonocytesandmicrog...
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ThecomplementinhibitoryproteinDAF(CD55)suppressesTcellimmunityinvivo
...sdemonstratedinarecentstudywherebycoengagementofCD3andthecomplementregulatorCD46,inthepresenceofIL-2,inducedanIL-10–secretingTregcellphenotypeinhumanCD4+Tcells(40).WefoundnodefectinnaiveDaf1–/–micewithregardtothenumberorfunctionoftheirnaturallyoccurringTregcells(Fig.6,E–G).Althoughthereduced...
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TheEpidemiologyofIntestinalMicrosporidiosisinPatientswithHIV/AIDSinLima,Peru
...tientwasaskedtoprovide3stoolspecimenson3separatedaysanda5-mLbloodspecimenforCD4lymphocytecount.Usingastructuredquestionnaire,thestudynursecollecteddataondemographics,thepresenceandseverityofgastrointestinalillnessatthetimeofinterview,treatmentanddiseaseprophylaxis,andpotentialexposures,includingpers...
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Cytolyticresponses:cadherinsputoutthefire
...1B,andhumandiscslarge(hDLG)protein(22,23).NKcellsandTcellsexpressthereceptorCD226,whichrecognizesnecl-5andnectin-2,andthereceptorCD96,whichrecognizesnecl-5(24–26).Inaddition,activatedNKcellsandTcellsup-regulatethereceptorCRTAM,whichbindstonecl-2(27).UnlikeKLRG1,however,thereceptorsspecificforthene...
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PreferentialmigrationofeffectorCD8+Tcellsintotheinterstitiumofthenormallung
...vatedbutnotnaiveTcellswereretainedwithinthelungsforaprolongedperiod.EffectorCD8+Tcellspreferentiallyegressedfromthepulmonaryvascularcompartmentintothenoninflamedpulmonaryinterstitium.Tcellretentionwithinthelungvasculaturewasleukocytefunctionantigen-1dependent,whiletheegressofeffectorTcellsfromthevas...
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PeripheralS-PhaseTCellsinHIVDiseaseHaveaCentralMemoryPhenotypeandRarelyHaveEvidenceofRecentTCellReceptorEngagement
...hFACSlysesolution(BDPharmingen)andstainedwithfluorescentsurfaceantibodiesforCD4,CD8,CD45RO,orCD62L(BDPharmingen).StainingofCCR7wasperformedbyuseofaprimaryIgMmouseantihumanCCR7antibody(BDPharmingen),followedbyagoatantimouseIgMphycoerythrin-conjugatedantibody.Controlstainincludedthegoatanti-mousesecon...
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Antiviraldefense:interferonsandbeyond
...tadvancesinourunderstandingofantiviralimmuneresponses(5).ThegroupscreenedforcDNAsthatcouldenhancetheactivationofanIFNregulatoryfactor3(IRF3)reporteraftertransfectionwithpolyinosinic–polycytidylicacid(polyI:C)—asyntheticdouble-stranded(ds)RNApolymerthatpotentlyinducestheproductionoftypeIIFNs.Thei...
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ThecorrelatesofdietaryintakeamongHIV-positiveadults
...ls,poornutritionalstatusisastrongpredictorofsurvival,evenaftercontrollingforCD4+cellcounts(12,13):aweightlossof66%ofidealbodyweightwaslinkedtothetimingofdeathinAIDSpatients(14).ItwasnotedthatHIVwastingsyndromewasassociatedwithdecreasedserumconcentrationsofvitaminA,folate,andcarotene(11).Nutritionals...
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Presenceofosteoclast-likemultinucleatedgiantcellsintheboneandnonostoticlesionsofLangerhanscellhistiocytosis
...TRAPpositivityontheCD68+MGCs(Fig.1A,left).TripleimmunofluorescentstainingforCD68,VNR,andCatKshowedthatallthebone(Fig.1B,left)andlymphnodelesions(notdepicted)withMGCswereVNR+andCatK+.Incontrast,oneoutofthreeskinlesionscontainingMGCswaspositiveforVNRandCatK(Fig.1B,right).Afurtherenzymecharacteristicof...
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HomeostaticmaintenanceofnaturalFoxp3+CD25+CD4+regulatoryTcellsbyinterleukin(IL)-2andinductionofautoimmunediseasebyIL-2neutralization
...onanyTcelluponactivation(11,12).Therefore,acriticalissueistodeterminewhetherCD25ismerelyanindicatorofthechronicallyactivatedstateofnaturalTregcellsorakeymolecule,specificandessentialfortheirgeneration,maintenance,orfunctionasanindispensablecomponentofthehighaffinityIL-2R(13).Ifthelatteristhecase,itn...
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EnhancementofimmunityintheelderlybydietarysupplementationwiththeprobioticBifidobacteriumlactisHN
...(postintervention).TheproportionofmononuclearleukocytesstainingpositivelyforCD3+(Tlymphocytes),CD4+(MHCII–restrictedTcells),CD25+(interleukin2receptor),andCD56+(NKcells)increasedsignificantlyafterconsumptionofB.lactisHN019(Table1).Afterthe3-wkwashoutperiodwithoutprobioticsupplementation(ie,atti...
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StoichiometryofthemurineTcellreceptor
...er(TCRorTCR)andasignal-transducingcomplex,consistingoftheCD3dimers(CD3and/orCD3)andaTCRhomodimer.TCRsignalingisrequiredforlineagecommitmentandrepertoireselectionduringdevelopment,formaintenanceoftheperipheralTcellpool,andfordifferentiationofnaiveTcellsintoeffectorandmemorycellpopulationsduringanimmu...
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EssentialroleoftheTcell–specificadapterproteinintheactivationofLCKinperipheralTcells
...hichexplainstheblockedERKresponse(Fig.1B).WealsoexaminedifTSAdwasrequiredforCD3/CD28-inducedintracellularcalciummobilizationthatisnecessaryfornucleartranslocationofNFATandcytokinegenetranscription(12).CalciummobilizationwasmonitoredwiththeuseoftheFura-2calcium-sensitivefluorescentdye(Fig.1C).Usingre...
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PTENinhibitsIL-2receptor–mediatedexpansionofCD4+CD25+Tregs
...edasasignificantincreaseinthenumbersofCD4+cellsexpressingtheactivationmarkerCD69(Figure2A).Aspreviouslyreported,theexpressionlevelsofCD25onCD4+TcellsdidnotvarysignificantlybetweenPTEN-Tmiceandwild-typelittermatecontrols(datanotshown).Figure2IsolationandanalysisofCD4+CD25+CD45RBloTregsfromPTEN-Tmice....
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Assessingtheeffectoffattyacidsonprostatecarcinogenesisinhumans:doesself-reporteddietaryintakerankprostaticexposurecorrectly?
...eRobertWoodJohnsonFoundation(MMFDP033363)andtheDepartmentofVeteransAffairs(RCD97-317).3Reprintsnotavailable.AddresscorrespondencetoVLFreeman,MidwestCenterforHealthServicesandPolicyResearch,ResearchService(151),DepartmentofVeteransAffairs,EdwardHines,Jr.Hospital,Hines,IL60141.E-mail:vfreema{at}wpo.it...
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Interleukin1andinterleukin18asmediatorsofinflammationandtheagingprocess
...effectsofIL-18havebeenreporteddependingonwhetherthediseaseismediatedbyCD4+orCD8+Tcells.Inhumans,Tcellsareresponsibleforthediseaseafterallogeneicbonemarrowtransplantation.AdministrationofIL-18torecipientmiceincreasessurvivalinCD4+-mediateddiseasebutresultsinworseningoftheCD8+-mediateddisease(78).Neut...