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UltrasoundStimulatesCyclooxygenase-2ExpressionandIncreasesBoneFormationthroughIntegrin,FocalAdhesionKinase,Phosphatidylinositol3-Kinase,andAkt
...nd51integrinwerepurchasedfromChemicon(Temecula,CA).Mousemonoclonalantibodyforv3waspurchasedfromDAKO(Glostrup,Denmark).RhodostominwaspurifiedfromvenomofCalloselasmarhodostoma(Huangetal.,1991).TheCOX-2promoterconstruct(pCOX2-Luc)wasagiftfromDr.H.R.Herschman(UniversityofCaliforniaatLosAngeles,LosAngele...
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ArgininevasopressinstimulatesH+-ATPaseinMDCKcellsviaV1(cellCa2+)andV2(cAMP)receptors
...12-10-6M)increasedtherateofpHirecoveryand[Ca2+]iinadose-dependentmanner.V1-orV2-receptorantagonistsimpairedtheeffectofAVPonbothprocesses,andDDAVP(10-12-10-6M;aV2-selectiveagonist)causedadose-dependentstimulationofthem.[Ca2+]iorcAMP(asincreasedby10-5Mthapsigarginor8-BrcAMP,respectively)alonehadnoeffe...
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DevelopmentalcontrolofCD8+Tcell–aviditymaturationinautoimmunediabetes
...differencescomparedwithV17.6,theleastusedV17elementby20weeks.(C)AveragemfiforV?8andCD8onTCR-?transfectantsexpressingthe8.3TCR-?chainandthe8.3–TCR-(V17.4+)or8.3-likeTCR-chains(usingV17.6orV17.5)(n=3cloneseach).(D)Tetramerbindingtotransfectants.(E)Flowcytometricanalysisof17.4/8.3?and17.5/8.3?transfe...
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Plasmaascorbicacidconcentrationsandfatdistributionin19068BritishmenandwomenintheEuropeanProspectiveInvestigationintoCancerandNutritionN
...kingstatus(never,former,orcurrent),andsocioeconomicgroup(I,II,IIIA,IIIB,IV,orV)formodels1to3;additionaladjustmentfordietaryascorbicacidandtotalenergyintakewasmadeformodel4.Thenumberofparticipantsinthe9waist-to-hipratiocategories(categories:0.70,0.70to0.75,0.75to0.80,0.80to0.85,0.85to0.9,0.9to0.95,0....
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心电图
...ndlebranchblockCRBBBQRS波組大於0.12秒V1出現RSR’波型(兔耳朵)V5orV6出現slurredS左分枝束阻斷(LBBB)LeftbundlebranchblockCLBBBQRS波組大於0.12秒rSinV1MshapeinV5作者：佚名
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LuteogenicHormonesActthroughaVascularEndothelialGrowthFactor-DependentMechanismtoUp-RegulateßandvßIntegrinsPromotingtheMigration
...viouslyreportedinhumanluteincellsinfunctioningcorporalutea.20Recently,mRNAforVEGFanditsreceptorsFlt-1andKDR/Flk-1wasdetectedinhumancorporaluteaduringthelutealphase.21WethereforeinvestigatedtheexpressionofselectedFN-bindingintegrinsonluteinizingGCs,thepotentialregulationofthatexpressionbyluteogenicho...
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A4-YearStudyoftheEpidemiologyofVibriocholeraeinFourRuralAreasofBangladesh
...itegelatinagar)at37°C.SuspectcolonieswereagglutinatedwithantiseraspecificforV.choleraeO1(InabaandOgawaserotypes)andforV.choleraeO139.PositivecolonieswereconfirmedasV.choleraebybiochemicaltesting,andthetwobiotypesofO1serogroup(eitherclassicalorElTor)weredifferentiatedbystandardmethods[25].Statistica...
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DifferentialDiagnosisofStrokeinaSettingofHighHIVPrevalenceinBlantyre,Malawi
...ficiencyhasbeensuggested,whereasotherpatientswerereportedtohavevasculopathyorvasculitis,eitherisolatedorinthecontextofmeningitis.5Thedifferentialdiagnosisofstrokeindevelopingcountriesincludesvariouscentralnervoussysteminfections(includingfungal,bacterial,andtuberculousmeningitis[TBM],cerebraltoxopla...
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Human1typeIVcollagenNC1domainexhibitsdistinctantiangiogenicactivitymediatedby1?1integrin
...ationofECs,proliferationofECs,tubeformationbyECs,andreattachmentofpericytesorvascularstabilization(3,4).Themajorcomponentofvascularbasementmembrane(VBM)istypeIVcollagen.Thereare6distinctgeneproducts,1–6,fortypeIVcollageninVBM(5).TypeIVcollagenplaysacrucialroleinangiogenesis(6-8).VBMconstituteanimp...
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第六节 肺功能检查
...8%Raw吸呼均值CmH2O/L.sCmH2O/L.sCmH2O/L.sFRC5.39LRV4.97LTLC7.6LCst1/cmH2ORV/TLC65.4%Cdyn1/cmH2OPEF2.7L/sP(A-a)O2:呼吸空气呼吸纯氧MmHgMmHgV500.4L/sV250.2L/sV50/V2502VD/VT% 检查结果肺活量中度降低，第一秒用力呼气容积占用力肺活量比值下降，最大通气量...
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StructuralBasisforCompetitionbetweenDrugBindingandKv1.3AccessorySubunit-InducedN-TypeInactivationofKv1.5Channels
...,1mMKCl,0.4mMCaCl2,0.33mMCa(NO3)2,1mMMgSO4,2.4mMNaHCO3,and10mMHEPES,pH7.4.Forvoltage-clampexperiments,oocyteswerebathedinamodifiedND96solutioncontaining96mMNaCl,4mMKCl,1mMMgC12,1mMCaC12,and5mMHEPES,pH7.6.Currentswererecordedatroomtemperature(23eC25°C)withstandardtwo-microelectrodevoltage-clamptechn...
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Integrin-MediatedTransformingGrowthFactor-ßActivationRegulatesHomeostasisofthePulmonaryEpithelial-MesenchymalTrophicUnit
...gainstv(L230),ß1(P5D2),ß3(AP3),vß5(P1F6),vß6(E7P6),orvß8(14E5).Antibodiesusedareindicatedbeloweachlane.Themigrationofthemolecularsizemarkersisindicatedontheright,andthesubunitscorrespondingtoeachbandareindicatedontheleft.Theexperimentsshownarerepresentativeofatleastthre...
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NewinsightsonhumanTcelldevelopmentbyquantitativeTcellreceptorgenerearrangementstudiesandgeneexpressionprofiling
...D34+CD38+CD1a–cells,andpeakedinCD34+CD38+CD1a+cells(Fig.1C).CompleteV1-J1orV2-J1rearrangementsweredetectedfirstinCD34+CD38+CD1a–cells,increasedtopeaklevelsinCD4+ISPcells,anddeclinedthereafterwhenthemajorityofthymocytesdifferentiatedfurtherintotheTCRlineage(Fig.1D).DuringTCRTcelllineagedevelopmen...
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CharacterizationoftheNaturalKillerT-CellResponseinanAdoptiveTransferModelofAtherosclerosis
...lationsfromthespleensofeitherC57BL/6(wild-type),CD1dC/C(NKTcell-deficient),orV14J18TCRtransgenic(NKTcell-enriched)miceandtheresultanteffectsonatherosclerosis.Nononphysiologicalexogenousantigenadministrationisinvolved,implyingthatthedifferencesnotedrelatetothepresentationofendogenouslipidantigenstoth...
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苦参的近代药理及临床研究进展
...1］。经实验证明，苦参碱有抗乙肝病毒作用，对RSV、CB、ORV、adv等病毒作用也较强，干扰素是机体对抗病毒感染最重要的淋巴因子，具有广谱抗病毒及免疫调节作用，苦参生物碱可诱生人-白细胞产生的α-干扰素，是其抗病毒感...
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SharedreactivityofV2negTcellsagainstcytomegalovirus-infectedcellsandtumorintestinalepithelialcells
...ess,buttheirpropensitytowardself-reactivityhasbeendemonstrated,asithasbeenforV9/V2Tcells.MHCclassI–relatedchainA(MICA;astress-inducedproteinexpressedonepithelialcells)andCD1careindeedabletostimulateV1+TcelllinesandtointeractwiththeirV1TCR(6,7).AnimportantfeatureofTcellsisthattheydonotrequireclassi...
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ImpactofHumanImmunodeficiencyVirusType1(HIV-1)GeneticDiversityonPerformanceofFourCommercialViralLoadAssays:LCxHIVRNAQuantitative,AMPLICO
...uantitativeassay(LCx),BayerVersantHIV-1RNA3.0(bDNA),RocheAMPLICORHIV-1MONITORv1.5(Monitorv1.5),andbioMerieuxNucliSensHIV-1QT(NucliSens).ThepanelincludedgroupM,groupO,andrecombinantvirusesbasedonsequenceanalysisofgagp24,polintegrase,andenvgp41.TheLCxHIVassayquantifiedviralRNAin97(100%)ofthesamples.In...
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CD45MOLECULEANDGENERATIONOFEPIDERMALγδTCELLSFROMMIC
...2，Cδgenesegment，thesecellswerenameddendriticepidermalTcells（DETC）orVγ3DETC． IncontrasttothefindingofKawaietal，outstudyshowednosignificantdifferenceinthedensity，morphologyandphenotypeofVγ3DETCbetweenCD45-deficientmiceandwild-typemice．Thediscrepancybetweenthetwogroupsmayberelat...
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Opticalcoherencetomographyanalysisofaxonallossinbandatrophyoftheopticnerve
...pletehemianopiawithonlyasmallinferiortemporalremainingoffieldwithI/3e,I/4e,orV/4etarget.AlleyeswithBArevealedcompletetemporalhemianopiaonHumphreyperimetry.FundoscopicexaminationrevealedBAoftheopticdiscandRNFLinall20eyeswithtemporalhemianopia.Table2Age,sex,studyeye,tumourdiagnosis,andvisualfielddefec...
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KininostatinAssociatesWithMembraneRaftsandInhibitsvβ3IntegrinActivationinHumanUmbilicalVeinEndothelialCells
...ligation.12D5alsodirectlybindstodomains2/3ofuPAR,whichservesasabindingsiteforVn.13HKathuscompetesforVninteractionwithbothvβ3integrinanduPAR.However,consideringthepotentinhibitionofHKaandD5oninvivoangiogenesis,theselectiveantiadhesiveactivity,whichistightlyregulatedbyVn,isnotsolelyresponsiblefor...
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上消化道出血的内镜治疗进展
...ction/endoscopicsclerotherapyofuppergastrointestinalpepticulcerhermorrhage,Orv-Hetil,1997;138:2593-2597TesngC,BurkeS,ConnorsP,GreenR,CarrlockeDL,Endoscopicbandligationfortreatmentofnon-varicealuppergastrointestinalbleeding,Endoscpy,1991;23:297ParraBA,TakahashiH,MendezPV,KojimaT,AksozK,KiriharaK,Palm...
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PolymorphismoftheHumanImmunodeficiencyVirusType2(HIV-2)ProteaseGeneandSelectionofDrugResistanceMutationsinHIV-2-InfectedPatientsTreatedwi
...mongthefourpatientstreatedwithNFV,twohadtheL90MchangeassociatedwithI54Mand/orV71I.Manyothertreatment-associatedchanges(n=25)wereselectedin13patients.Valineorleucineataminoacid84wasfoundinfourofthesixpatientsthatreceivedasecondPI-containingregimen.Inourstudy,weanalyzed123HIV-2PRsequencesfrom94PI-naiv...
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Objectiveperimetryusingthemultifocalvisualevokedpotentialincentralvisualpathwaylesions
...dwhohadvariousformsofhomonymousorbitemporalfieldlossfromlesionsoftheposteriorvisualpathway(pituitarytovisualcortex).Allsubjectshadsubjectivevisualfields(HumphreyvisualfieldSitastandard24-2),and17/19hadsomeformofneuroimaging(computedtomography(CT)ormagneticresonanceimaging(MRI))available.Threeofthesu...
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FunctionalrescueofvasopressinV2receptormutantsinMDCKcellsbypharmacochaperones:relevancetotherapyofnephrogenicdiabetesinsipidus
...(23).Inthesecells,V2R-GFPwaslocalizedandregulatedascanbeanticipatedtooccurforV2Rinvivo.Moreover,wefoundthatseveralV2RmutantsinNDIstablyexpressedinMDCKcellsareERretained(24).TodeterminewhichCPAnislikelytheoptimalpharmacologicalchaperonetorelieveNDIinpatients,wethoroughlytestedaV1receptorantagonist,am...
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RelationshipBetweenArterialStiffnessandCognitiveFunctioninElderlySubjectsWithComplaintsofMemoryLoss
...:normalcognitivefunction,mildcognitiveimpairment(MCI),Alzheimerdisease(AD),orvasculardementia(VaD).Arterialstiffnesswasevaluatedbycarotid-femoralpulsewavevelocity(PWV)measurementusingComplior.Results—Inthispopulation,78±8yearsofage(women64%),ADwaspresentin41%,VaDin6%,MCIin27%,and26%ofsubjectshadn...
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AntiviralTcellresponses:phalanxormultiprongedattack
...unodominantepitopeglycoproteinB(gB498-505)ofherpessimplexvirus(HSV)-1useV10orV8TCRs.TheresponseislessvigorousandlessdiverseinC57BL/6(B6)micethanincoisogenicbm-8(B6.C-H-2bm8)mice,whichdifferonlybyafewaminoacidresiduesinthepeptidebindinggrooveoftheMHC-IKbmolecule.B6micesuccumbtoviralinfectionat25-fold...
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SignalingforNKTcelldevelopment:theSAP–FynTconnection
...(V14-J18inmice[V14i],V24-J18inhumans)combinedwithoneofthreeVsegments(V8,V2,orV7inmice,V11inhumans).ThesecanonicalTCRsrecognizetheself-glycosphingolipidiGb3(1),whichfullyactivatesmatureNKTcellsbothinvitroandinvivoduringsomemicrobialinfections(2).Giventhestrongself-agonistactivityofiGb3,itisparadoxica...
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Ligationofprotease-activatedreceptor1enhancesv?6integrin-dependentTGF-?activationandpromotesacutelunginjury
...ev?6integrin.(A)Micewerepretreatedwithcontrolnonblockingantibody(whitebars)orv?6blockingantibody(blackbars)beforeventilationat24ml/kgandintratrachealinstillationwithPAR1-specificpeptide(TFLLRN),vehicle(HBSS),orpeptidecontrol(FTLLRN).ELWwasmeasuredasthewet-to-dryweightratioofexcisedlungs.(B)Micewerep...
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Vasopressin-2-receptorantagonismaugmentswaterexcretionwithoutchangesinrenalhemodynamicsorsodiumandpotassiumexcretioninhumanheartfailure
...dpressure,orincreasedplasmaosmolality.AVPactsviathreereceptortypes:V1A,V1B(orV3),andV2.V1Areceptorsarelocatedinvascularsmoothmusclecells,wheretheymediatevasoconstriction,andinthemyocardium,wheretheymayaffecthypertrophy(24).V1Breceptorsarelocatedintheanteriorpituitaryandmediateadrenocorticotropinrele...
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AntidiarrhealEffectsofL-HistidineSupplementedRice-BasedOralRehydrationSolutionintheTreatmentofMaleAdultswithSevereCholerainBangladesh:ADou
...alcharacteristicslistedintable1.PatientsinfectedwitheitherV.choleraeElTorO1orV.choleraeO139didnotdifferintheirpretreatmentcharacteristics,numberofstools,andoutputofstoolafterinitiationofORStreatment.Thedeclineinstooloutputstartedfromthefirst8-hperiodafterinitiationofORStreatment,butthedifferenceswer...