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MechanismsimposingtheVbiasofV14naturalkillerTcellsandconsequencesformicrobialglycolipidrecognition
...thetichomologueofmicrobial-glycuronosylceramides,wasrecognizedbyabroadersetofVchains,includingthebiasedNKTsetbutalsoV6,V9,V10,andV14.Thesesurprisingfindingsdemonstratethat,whereasV8,V7,andV2representtheoptimalsolutionforrecognitionofendogenousligand,manyVchainsthatarepotentiallyusefulfortherecogniti...
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OskarKokoschkaandAugusteForel
...ulyfantastic,withastrongemphasisonmysticism,cryptotheology,andtheimportanceofvisions.2Forel,ontheotherhand,isonthesurfacemoresober,yetthetextteemswithremarkableideasandcross-connections,particularlybetweenneuroanatomy,thesociallifeofants,addiction,sexuality,andtheBah’aifaith.3AugusteForelAugusteFo...
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SugarsInhibitExpressionoftheRugosePhenotypeofVibriocholerae
...ichonlysporadicornocasesofcholeracanbedetected(2,3).AlthoughepidemicstrainsofV.choleraecanpersistforyearsinnaturalwaters,theunderlyingbasisforthesurvivalofV.cholerae,particularlyduringinterepidemicperiods,isnotwellunderstood.AsoriginallydescribedbyWhitein1938(11),V.choleraecanassumea“rugose“phen...
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CharacterizationofvasopressinV2receptormutantsinnephrogenicdiabetesinsipidusinapolarizedcellmodel
...r(V2R).ForthedevelopmentofatailoredtherapyforNDI,knowledgeofthecellularfateofV2Rmutantsisneeded.Itwouldbeusefulwhenthisfatecouldbepredictedfromthelocationandtypeofmutation.Toidentifysimilaritiesanddifferencesinlocalization,maturation,stability,anddegradationofCOOH-terminalGFP-taggedV2Rmutants,westab...
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Objectiveperimetryusingthemultifocalvisualevokedpotentialincentralvisualpathwaylesions
...ldresultsandCT/MRIfindingswasdonetodeterminetherelationbetweenthetwomethodsofvisualfieldmappingandanyrelationwiththeanatomicallocationofthelesionandthemVEPresults.Results:Inallhemianopictypecases(12)thedefectwasdemonstratedonthemVEPandshowedgoodcorrespondenceinlocationofthescotoma(ninehomonymousandt...
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人脐血干细胞移植治疗大鼠脑出血的实验研究
...ortwodays.Intracerebralhemorrhage（ICH）modelswasinducedinratsbyinjectionofVⅡtypecollagenaseintotherightglobuspallidusandtheHUCBCweretransplantedintothetailvein24hourslater.NeurologicalSeverityScores（NSS）testswereundertakenandimmunohistochemistrymethodwasusedtocheckthemigrationanddifferentia...
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AbdominalAorticAneurysmRuptureIsAssociatedWithIncreasedMedialNeovascularizationandOverexpressionofProangiogenicCytokines
...ofsmallerdiameter(P0.001)andmoreimmaturemicrovessels(P0.001).mRNAexpressionofv-integrin,vascularendothelialgrowthfactor,vascularendothelial-cadherin,monocytechemoattractantprotein-1,andvimentinwasincreased(P0.05)inruptureedgebiopsysamples.Conclusions-Thisstudydemonstratedincreasedmedialneovasculariz...
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FunctionalrescueofvasopressinV2receptormutantsinMDCKcellsbypharmacochaperones:relevancetotherapyofnephrogenicdiabetesinsipidus
...tor(V2R)isamajorcauseofcongenitalnephrogenicdiabetesinsipidus(NDI)andrescueofV2Rmutantsbynonpeptideantagonistsmayrestoretheirbasolateralmembrane(BM)localizationandfunction.However,thecriteriaforefficientfunctionalrescueofGprotein-coupledreceptor(GPCR)mutantsatclinicallyfeasibleantagonistconcentratio...
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PandemicSerovars(O3:K6andO4:K68)ofVibrioparahaemolyticusAssociatedwithDiarrheainMozambique:SpreadofthePandemicintotheAfricanContinent
...ul,KoreaWorldHealthOrganization,Geneva,SwitzerlandABSTRACTForty-twoepisodesofVibrioparahaemolyticusinfectionsweredetectedinBeira,Mozambique,fromJanuarytoMay2004.Themajorityoftheisolates(81%)belongedtothepandemicserovars(O3:K6andO4:K68)ofV.parahaemolyticus.Thepandemicserovarswerepositivebygroup-speci...
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Strand-biaseddefectinC/GtransversionsinhypermutatingimmunoglobulingenesinRev1-deficientmice
...lonallyrelatedsequences(TableS2).Datarepresentmutationsbetweencodons13and97ofV1(23)afterexclusionofputativelyclonallyrelatedmutants.Onlinesupplementalmaterial.TableS1isacompositionofBandTcellsubsetsofprimaryandsecondarylymphaticorgansofwild-typeandRev1–/–mice.Noqualitativedifferencesarefoundbetw...
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Vasopressin-2-receptorantagonismaugmentswaterexcretionwithoutchangesinrenalhemodynamicsorsodiumandpotassiumexcretioninhumanheartfailure
...nadouble-blind,placebo-controlledstudy,theseinvestigatorsexaminedtheeffectsofV2antagonisminhumanswithCHF.Theyfoundthat,onthefirstday,V2-blockedpatientshadanincreaseinurinevolume,andthiswasaccompaniedbyadecreaseinweightthatwasmaintainedthroughoutthestudy.Inasubsequentstudy,AcuteandChronicTherapeuticI...
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SensitivitytoBronchodilationinChronicObstructivePulmonaryDisease
...agues(15).After2minofwarm-up,walkingspeedwassetatthespeedcorrespondingto80%ofV·O2peak,aspredictedfromincrementalshuttlewalking.Beforeeachendurancetest,patientswereaskedtowalkorcycleforaslongaspossible.Noencouragementwasprovidedduringtheteststoavoidanypotentialconfoundingeffectonexerciseperformance(...
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InhibitionofadeninenucleotidetranslocatorporefunctionandprotectionagainstapoptosisinvivobyanHIVproteaseinhibitor
...nceofNFV/RIT.SystemictreatmentwithSEBresultsinshockandtheselectiveapoptosisofV-?8–positiveTcells(21).WhencoadministeredwithD-galactosamine(D-gal),SEBinjectionalsoresultsindeath(22,23).FollowingadministrationofSEB,apoptosiswasmeasuredbyTUNELassayintheV-?8andV-?3subsetsofsplenocytes.Fourhoursfollowi...
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TheEenhancercontrolsthegenerationofCD4–CD8–TCR-expressingTcellsthatcangiverisetodifferentlineagesofTcells
...racterizedbysequentialrearrangementandexpressionofTCRgenes(2).RearrangementofvariableandTCRgenesegmentsbeginsattheDN2stageand,ifproductive,resultsinCD4–CD8–(DN)cellsthatexpressTCRonthecellsurface.RearrangementofvariableTCRgenesegmentssetsinslightlylaterand,alsoifproductive,resultsinthesurfaceexp...
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RelativecontributionsofCa2+mobilizationandinfluxinrenalarteriolarcontractileresponsestoargininevasopressin
...fferencesexhibitedbyafferentandefferentarteriolesregardingthefunctionalroleofVGCCsinelicitingvasoconstriction(35).TheroleofCa2+mobilizationinelicitingconstrictionofafferentandefferentarteriolesmightalsoreflectdisparitiesinagonist-specificsignalingeventswithinthesecells,althoughfewagonistshavebeenstu...
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HumanCervicovaginalLavageFluidContainsanInhibitorofHIVBindingtoDendriticCellSpecificIntercellularAdhesionMolecule3GrabbingNonintegrin
...isbound,internalized,andtransferredtoCD4+Tcells,suggestingaTrojanhorsemodelofviraltransmission[9,12].ThetransferofgreenfluorescentproteinlabeledvirusfromDCstoTcells,alongwiththerecruitmentofHIVreceptorstothesiteofcontactbetweencells,hasbeenobserved[13].Huetal.havedescribedtherapidmigrationofDC-SIGNr...
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Associationbetweenglycatedhemoglobinanddietandotherlifestylefactorsinanondiabeticpopulation:cross-sectionalevaluationofdatafromthePotsdam
...tionusereferredtothe4wkbeforetheinterview.StatisticalanalysisAfirstanalysisofvarianceindicatedthatthevariablebatchnumber,100randomlyselectedsamplesoftheimmunoassay,wassignificantlyrelatedtoHbA1cvaluesevenafteradjustmentforothervariablessuchasage,sex,andBMI,indicatingsystematicanalyticdifferencesbetw...
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?3-IntegrinRegulatesVascularEndothelialGrowthFactor-A–DependentPermeability
...yWesternblotanalysis,flowcytometry,andimmunofluorescence).CirculatinglevelsofVEGF,baselinebloodvesselleakage,andleakageinresponsetoanacuteinflammatorystimuluswereidenticalinwild-typeand?3-nullmice.However,VEGF-A–inducedleakagewasabolishedin?3-nullmicebytheinhibitionofFlk-1,indicatingthattheelevate...
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A4-wkinterventionwithhighintakeofcarotenoid-richvegetablesandfruitreducesplasmaC-reactiveproteininhealthy,nonsmokingmen
...ail:bernhard.watzl{at}bfe.uka.de.ABSTRACTBackground:Whetherdifferentintakesofvegetablesandfruitmodulateimmunologicmarkersiscurrentlynotknown.Objective:Weinvestigatedtheeffectsoflow,medium,andhighintakesofvegetablesandfruitonmarkersofimmunefunctions,includingnonspecificmarkersofinflammation.Design:In...
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EndothelialCellActivationbyIL-1?inthePresenceofFibrinogenRequiresV?3
...ociationbetweenv?3andIL-1R.Dualimmunofluorescencealsorevealedcolocalizationofv?3andIL-1RonlywhenthecellswereexposedtobothfibrinogenandIL-1?.Conclusion—TheenhancedNOproductionbyECsinthepresenceoffibrinogen-boundIL-1?requiresthecoordinatedeffectsofcolocalizedV?3andIL-1R.Theabilityoffibrinogen-boundI...
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KininostatinAssociatesWithMembraneRaftsandInhibitsvβ3IntegrinActivationinHumanUmbilicalVeinEndothelialCells
...ithuPARandcaveolin-1,whichwasaccompaniedbyanincreaseinligandbindingaffinityofvβ3integrin.TheseeventswereinhibitedbyD5.Consistently,D5suppressedspecificvβ3integrin-mediatedECadhesionandspreadingaswellassmallguanosinetriphosphataseRac1activation.Conclusions—D5bindstoECsviamembranerafts...
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GenomicScreeninginVivoRevealstheRolePlayedbyVacuolarH+ATPaseandCytosolicAcidificationinSensitivitytoDNA-DamagingAgentsSuchasCisplatin
...itivityofthemutantstohydroxyurea(aDNA-alkylatingagent)andUVirradiation.LossofV-ATPasedoesnotaffectDNArepair,becausedoublemutantsdefectiveforV-ATPasefunctionandDNArepairpathwaysweremoresensitivetocisplatinthanthesinglemutants.V-ATPasemutantsaremorepronetoDNAdamagethanwild-typecells,indicatedbyenhance...
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Age-dependentregulationofvasopressinV1areceptorsinpreglomerularvesselsfromthespontaneouslyhypertensiverat
...ypertensionseemstobemediatedthroughtheV1areceptors.IntrarenaladministrationofV1aagonistproducesvasoconstriction,saltandwaterretention,andhypertension(12).BlockingthisreceptorwithaV1a-specificantagonistdelaysthehypertensivedevelopmentinSHR(4,33).WaterretentionthroughV2stimulationdoesnotcontributeinth...
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v3-dependentcross-presentationofmatrixmetalloproteinase–2bymelanomacellsgivesrisetoanewtumorantigen
...couldbeprocessedandassociatedtoHLA-classIinmelanomacells,westudiedtheeffectofvariousinhibitorsofpeptidepresentationpathwaysonMMP-2peptidepresentationbytwospontaneouslypresentingcellline(M134andFM29)andbytheM102celllineinducedtopresenttheepitopefromanexogenoussupply.Monodansylcadaverine,aninhibitorof...
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DownregulationofvasopressinV2receptorpromoteractivityviaV1areceptorpathway
...R,respectively)distributeinthecollectingductofthekidney.AlthoughthefunctionofV2RmediatingtheantidiureticeffectofAVPhasbeeninvestigatedindetail,theroleofV1aRinthecollectingductshasnotbeenelucidated.Inthepresentstudy,wehaveinvestigatedtheroleoftheV1aRpathwayinV2Rpromoteractivity.Weclonedthe5‘-flanki...
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ATP6V0CCompeteswithVonHippel-LindauProteininHypoxia-InducibleFactor1(HIF-1)BindingandMediatesHIF-1ExpressionbyBafilomycinA1
...proteinofbafilomycin,hasbeenfoundtointeractwithotherstructuresindependentlyofV-ATPase,includinggapjunctioncomplexesandmediatophores(Finbowetal.,1994).ItalsoformsacomplexwithpapillomavirusE5oncoproteinplusplatelet-derivedgrowthfactor-receptor(Goldsteinetal.,1992)orwith1integrin(Leeetal.,2004).Thus,AT...
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TCellRegulationasaSideEffectofHomeostasisandCompetition
...ecellsattheendpointoftheexperiment.ANDTcellswereidentifiedbasedonexpressionofV11.(B)Equalnumbers(2x105)ofCD45RBhiandCD45RBloorANDRag-/-CD4Tcells(allH-2b)wereadoptivelytransferredintoRag-/-H-2bhosts.BodyweightandpercentageofTcellsinbloodandlymphoidorgansweremonitoredasinA. MonitoringofCD4Tcellexpan...
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TCellRegulationasaSideEffectofHomeostasisandCompetition
...ecellsattheendpointoftheexperiment.ANDTcellswereidentifiedbasedonexpressionofV11.(B)Equalnumbers(2x105)ofCD45RBhiandCD45RBloorANDRag-/-CD4Tcells(allH-2b)wereadoptivelytransferredintoRag-/-H-2bhosts.BodyweightandpercentageofTcellsinbloodandlymphoidorgansweremonitoredasinA. MonitoringofCD4Tcellexpan...
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HIV-1fusionpeptidetargetstheTCRandinhibitsantigen-specificTcellactivation
...Tcells.(B)ColocalizationofFP-RhowiththeCD4andTCRmolecules.(C)ColocalizationofV2E-RhowiththeTCR.(D)FRETbetweenFP-RhoorV2E-RhoandFITC-labeledantibodiestoTCRisconfirmedbyincreaseofFITC(donor)intensityafterbleachingofaspotofrhodamine(acceptor)at543nm.Scalebar:5μm.TheTCR,CD3,andCD4molecules,amongotherco...
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APETx1fromSeaAnemoneAnthopleuraelegantissimaIsaGatingModifierPeptideToxinoftheHumanEther-a-go-go-RelatedPotassiumChannel
...ing10µMAPETx1.TheformatisthesameasFig.3C,buttheVtvalueisvariedbecauseofV0.5shiftcausedbythemutations(markedforeachmutanttype).C,summaryofdegreeofV0.5shiftcausedby10µMAPETx1;n,numberofmeasurements.One-wayANOVA,p=0.004;*,p<0.05versusWT.Fig.6.Effectsofthiol-modifyingMTSreagentsontheS517C,E5...