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ATV-NDV治疗针对肿瘤细胞具有独特优势
...）企业副总裁李刚毅硕士在介绍，新城疫（鸡瘟）病毒（NDV）以“出芽”方式增殖的细胞非溶解性毒株，只感染人肿瘤细胞，不感染人正常细胞，且对人体没有神经毒作用。他介绍了以NDA修饰的自体肿瘤疫苗（ATV-NDV）治疗肿瘤...
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病毒激活巨噬细胞产生的一氧化氮与细胞毒的关系
...胞毒活性 摘 要 目的：确定新城鸡瘟病毒Losota系(NDV-L)能否诱导小鼠腹腔巨噬细胞(PEMφ)产生一氧化氮(NO)以及该NO与小鼠PEMφ介导的细胞毒作用是否有依赖关系。方法：采用Griess、FACS分析和噻唑蓝(MTT)方法。结果：NDV-L与PEMφ...
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新城疫病毒对胃癌细胞抗原修饰作用的研究
【摘要】目的研究新城疫病毒（NDV）的抗原修饰作用在抗原致敏树突细胞（DC）与细胞因子诱导的杀伤细胞（CIK）共培养NDV体外杀伤胃癌（SGC7901）细胞中所发挥的影响。方法从正常人外周血单个核细胞诱导DC、CIK细胞，用胃癌...
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体外单核巨噬细胞诱生IFN-α的因素分析
...学微生物学教研室南京210029关键词：M-CSF；IFN-γ；IFN-α；NDV；单核细胞 摘 要 观察了M-CSF预处理、IFN-γ预处理、长程培养及新城疫病毒（new-castlediseasevirus，NDV）刺激4个因素对正常人外周血单核细胞体外产生IFN-α水平的影响...
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NDV修饰乳腺癌肿瘤细胞免疫治疗后20例近期副反应观察
...却有较大的毒副反应。而新城疫病毒（NewCastleDiseaseVirus，NDV）是不引起人类疾病的选择性禽类病毒，其应用于恶性肿瘤的生物治疗，具有来源方便、容易管理、安全可靠等优点，是一种有前途的抗肿瘤制剂［1］。我们在临床上...
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负载抗原DCCIK细胞对NDV修饰抗原胃癌细胞杀伤作用
...的探讨负载SGC7901细胞抗原的DCCIK细胞对新城疫病毒(NDV)修饰SGC7901细胞的杀伤作用，探索胃癌新的治疗方法。方法取健康成人外周血分离单个核细胞，用于诱导培养树突状细胞(DC)及细胞因子诱导的杀伤细胞(CIK细胞)。用反复...
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新城疫基因工程疫苗研究
...养长期内多次进行免疫接种，仍有可能感染新城疫病毒（NDV）强毒，造成非典型ND的流行。因疫苗的广泛使用，从现场鸡群中分离到NDV或检测到NDV抗体，均不能作出ND感染的诊断，这就给ND的诊断和清群带了困难。常规弱毒疫苗和...
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对糖尿病多发神经病变音叉足以达到筛查目的
...的两个版本和荷兰NederlandseDiabetesFederatie-CentraalBeleidsOrgaan(NDV/CBO)的四个版本。Meijer医生及其研究组在9月的《糖尿病治疗》（DiabetesCare2005;28:2201-2205）杂志上指出，ICDF1包括128-Hz音叉试验、拇趾针刺痛感检查、跖面单纤丝试验和跟...
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Cyclicesotropiaandthetreatmentofover-elevationinadductionandV-pattern
...cularitywasreachedinallthreepatients.Moreover,theover-elevationinadductionandV-patterncompletelyresolved.Conclusions:V-patternandbilateralover-elevationinadductionwasseeninallpatientswithcyclicesotropiainthestagethatfusionwasdisrupted.Possibleexplanationsfortheoriginofthesepatternsarediscussed.Strab...
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副粘病毒融合蛋白分子上特异性细胞融合作用位点的初步确定
...得突变株的细胞融合功能与野毒株相同；将新城疫病毒(NDV)F和副流感病毒(PIV)F在膜穿入部分和躯干部分交接处交换时，不影响细胞融合功能；进一步将F2进行交换时，也不影响细胞融合功能；而将F的头部进行交换时，则检测不到...
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Loop-MediatedIsothermalAmplificationforRapidDetectionofNewcastleDiseaseVirus
...(LAMP)assayfortherapid,simple,andsensitivedetectionofNewcastlediseasevirus(NDV)directlyfromcultureisolatesaswellasclinicalsamples.Byusingonesetofspecificprimerstargetingthefusionproteingene,theLAMPassayrapidlyamplifiedthetargetgenewithin2h,requiringonlyaregularlaboratorywaterbathorheatblockforreacti...
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多糖的免疫调节作用研究进展
...清IL-2R的水平［10］。将给予灰树花多糖的小鼠腹腔注射NDV（新城鸡瘟病毒）后，结果显示灰树花多糖能促进NDV诱生干扰素，这可能是灰树花多糖抗病毒感染的作用机理。树舌多糖本身对体外培养的人扁桃体细胞干扰素的诱生无...
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EssentialroleofIPS-1ininnateimmuneresponsesagainstRNAviruses
...fibroblastcell;MOI,multiplicityofinfection;MVA,modifiedvacciniavirusAnkara;NDV,Newcastlediseasevirus;PEC,peritonealmacrophage;polyI:C,polyinosinic-polycytidylicacid;PRR,patternrecognitionreceptor;RIG-I,retinoicacid–induciblegeneI;SeV,Sendaivirus;TLR,Toll-likereceptor;VSV,vesicularstomatitisvirus;T...
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二、抗独特型抗体在医学中的应用
...质炎病毒的感染。Tanaka等在BALB/c小鼠中用抗新城疫病毒（NDV）血凝素的抗体诱导出抗Id抗体，在无病毒抗原存在的情况下，这种抗Id抗体可刺激小鼠产生抗NDV抗体。Lathey用单纯疱疹病毒（HSV）糖蛋白D特异的McAb制备了抗Id抗体，并...
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MonoclonalAntibodyRoutinelyUsedToIdentifyAvirulentStrainsofNewcastleDiseaseVirusBindstoanEpitopeattheCarboxyTerminusoftheHemagglutinin-N
DepartmentofMolecularGeneticsandMicrobiology,PrograminImmunologyandVirology,UniversityofMassachusettsMedicalSchool,Worcester,Massachusetts01655ABSTRACTNewcastlediseasevirus(NDV)strainsareclassifiedashavinghigh(velogenic),intermediate(mesogenic),orlow(lentogenic)pathogenesisandvirulenceinchickens.Rec...
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冻干精制人白细胞干扰素制造及检定规程
...应达到90%以上。 1.1.2诱生病毒 采用新城疫病毒（NDV）F株或仙台病毒，经检定血凝效价达到适宜滴度，方可投产。 1.1.3培养液 1.1.4制造工作室的设置，应符合工艺流程要求。冷库及各种生产用具，必须专用，严禁...
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LungVolumeReductionSurgeryforEmphysema:CorrelationofCTandV/QImagingwithPhysiologicMechanismsofImprovementinLungFunction
...USSIONREFERENCES：PURPOSE:Tocomparetherelationshipbetweencomputer-derivedandvisuallyassessedventilation-perfusion(V/Q)scintigraphyandcomputedtomographic(CT)scoresinevaluatingdiseaseseverityanddistributioninidentifyingoptimalcandidatesforlungvolumereductionsurgery(LVRS)andtocorrelatetheseradiologici...
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LungVolumeReductionSurgeryforEmphysema:CorrelationofCTandV/QImagingwithPhysiologicMechanismsofImprovementinLungFunction1
...USSIONREFERENCES：PURPOSE:Tocomparetherelationshipbetweencomputer-derivedandvisuallyassessedventilation-perfusion(V/Q)scintigraphyandcomputedtomographic(CT)scoresinevaluatingdiseaseseverityanddistributioninidentifyingoptimalcandidatesforlungvolumereductionsurgery(LVRS)andtocorrelatetheseradiologici...
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刘莎符州:黄芪的免疫调节作用及其临床应用
...黄芪不仅能促进小鼠和人外周血白细胞对新城鸡瘟病毒(NDV)或仙台病毒(SendalV)诱生IFN-0~，促进环己亚胺、放射菌素D在人肺二倍体细胞上引起IFN-p超诱导反应，而且可诱导小鼠脾细胞产生IFN-γ。1．1．2促进抗体和补体生成：黄芪水...
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Variablegp43SecretionbyParacoccidioidesbrasiliensisClonesObtainedbyTwoDifferentCultureMethods
...oldSpringHarbor,N.Y.Singer-Vermes,L.M.,M.C.Ciavaglia,S.S.Kashino,E.Burger,andV.L.Calich.1992.Thesourceofthegrowth-promotingfactor(s)affectstheplatingefficiencyofParacoccidioidesbrasiliensis.J.Med.Vet.Mycol.30:261-264.Towbin,H.,T.Staehelin,andJ.Gordon.1979.Electrophoretictransferofproteinsfrompolyacr...
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ADisintegrinandMetalloprotease33PolymorphismsandLungFunctionDeclineintheGeneralPopulation
...1(G/A),Q-1(C/T),S_1(Val-Iso),S_2(G/C),ST+5(A/G),T_1(Met-Thr),T_2(Pro-Ser),andV_4(C/G).TheSNPcausesanaminoacidchangewhenthisisindicatedbetweenparentheses;otherwise,thebasechangeisshown.Seetheonlinesupplementforadescriptionofthegenotypingprotocol;sequencesofprimersandprobesarelistedinTableE1oftheonlin...
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流行性感冒中医药防治的临床与实验研究进展
...凝素特异性单克隆抗体6E6株杂交瘤细胞和抗新城疫病毒(NDV)血凝素与神经氨酸酶单克隆抗体10B1，株杂交瘤细胞，收集细胞培养上清，以血凝抑制试验和间接免疫荧光试验(IFA)检测单抗水平，探讨转化型人参皂苷对单克隆抗体生成...
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Antiviraldefense:interferonsandbeyond
...anscribeddsRNAandisrequiredfortheinvivoresponsetoVSV,Newcastlediseasevirus(NDV),Sendaivirus,andinfluenzavirus(14,15).MDA5,bycontrast,istheprincipalreceptorforpolyI:Candisessentialfortheantiviralresponsetothepicornavirusencephalomyocarditisvirus(EMCV)(15,16).Tworecentstudies,oneintheJulyissueoftheJEM...
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Nutrientintakeincommunity-dwellingadolescentgirlswithanorexianervosaandinhealthyadolescents
...significantlymoregirlswithANmettheDietaryReferenceIntakeforcalcium(P=0.01)andvitaminD(P=0.02)fromsupplementuse.Fatintakepredictedghrelin,insulin,andIGF-Iconcentrations;carbohydrateintakepredictedadiponectin.Restingenergyexpenditurewaslower(P0.0001)andleisureactivitylevelshigherintheANgroup.Conclusio...
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Effectoffolateoversupplementationonfolateuptakebyhumanintestinalandrenalepithelialcells
...aziriandHamidMSaid1FromtheDepartmentofMedicine,DivisionofNephrology(BA,ZMM,NDV,andHMS)andtheDepartmentofPhysiologyandBiophysics(NDVandHMS),UniversityofCalifornia,Irvine,CA,andtheDepartmentofVeteransAffairsMedicalCenter,LongBeach,CA(BAandHMS)2SupportedbygrantsfromtheNationalInstitutesofHealth(DK58057...
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KininostatinAssociatesWithMembraneRaftsandInhibitsvβ3IntegrinActivationinHumanUmbilicalVeinEndothelialCells
...ngaffinity.WOW-1isaFabfragmentwhichspecificallyrecognizesactivatedvβ3andvβ5integrins.19VEGFstimulatedWOW-1bindingtoECs(Figure3E),supportingthatVEGFincreasesactiveintegrinrecycling.D5significantlyinhibitedtheincreaseinWOW-1bindingtoECsstimulatedbyVEGF,butdidnotaffecttheoverallexpressionofv&...
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Mispolarizationofdesmosomalproteinsandalteredintercellularadhesioninautosomaldominantpolycystickidneydisease
...culture(32).After7daysatfullconfluence,cytokeratinexpressionwaspredominantandvimentinexpressionwasminimalinnormalkidneyepithelialcells(Fig.3A,NK).Incontrast,vimentinremainedhighlyexpressedinADPKDcellsevenafterlong-termcultureandexhibitedaconspicuousfilamentouspatternthroughoutthecytoplasmofADPKDcell...
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SecretedProteinAcidicandRichinCysteine(SPARC)InhibitsIntegrin-MediatedAdhesionandGrowthFactor-DependentSurvivalSignalinginOvarianCancer
...enuationofcellsurfaceexpressionandclusteringofv-integrinsubunit,vß3-andvß5-heterodimers,andß1-subunit,albeittoalesserextent,inovariancancercells.Moreover,SPARCsignificantlysuppressedbothanchorage-dependentand-independentactivationofAKTandmitogen-acti-vatedproteinkinasesurvivalsigna...
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EphA2:expressionintherenalmedullaandregulationbyhypertonicityandureastressinvitroandinvivo
...isionofNephrologyandHypertension,OregonHealth&ScienceUniversityandthePortlandVeteransAffairsMedicalCenter,Portland,OregonSectionofNephrology,DepartmentofMedicine,BostonUniversitySchoolofMedicineandBostonMedicalCenter,Boston,MassachusettsDepartmentofPathology,JohnsHopkinsUniversitySchoolofMedicine,Ba...
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Entryofparainfluenzavirusintocellsasatargetforinterruptingchildhoodrespiratorydisease
...proximately150–400nmindiameter,andhaveanenvelopecomposedofhostcelllipidsandviralglycoproteinsderivedfromtheplasmamembraneofthehostcellduringviralbudding.TheHPIVgenomeissingle-stranded,negative-senseRNAthatmustbetranscribedintomessage-senseRNAbeforeitcanbetranslatedintoprotein.Likeallnegative-stran...