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多灶性运动神经病2例临床分析
...；神经电生理多灶性运动神经病（multifocalmotorneuropathy，MMN）又称多灶性脱髓鞘性运动神经病［1］，是一种周围神经病，病因不明，可发生于任何年龄，以青年男性多见，临床表现与运动神经元病（MND）有相似之处。现将我科近...
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中国研究者研究注意缺陷多动障碍患儿哌甲酯治疗前后的脑诱发电位
...儿童（正常对照组）的事件相关电位P瑚、失配性负波（MMN）、关联性负变（CNV）和脑干听觉反应（ABR），对其中31例ADHD患儿于哌甲酯（10—15mg口服）治疗4个月后再次检测。结果发现（1）与正常对照组比较，ADHD组P300-N2靶潜伏期...
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UseofaBJAB-DerivedCellLineforIsolationofHumanHerpesvirus8
...3cellswereseededataconcentrationof4x105cells/mlinthepresenceof0.3,1.5,3,or6mMn-butyrate(Sigma-Aldrich)or20ng/mlofphorbol12-myristate13-acetate(TPA;Sigma-Aldrich).Supernatantswereprocessedat48and72haftertreatment,asdescribedbelow,andvirusproductionwasevaluatedbyasemiquantitativePCR.Processingofvirals...
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运动神经元病患者神经传导阻滞的研究
...(MND组)、60例正常人(正常组)，14例多灶性运动神经病患者(MMN组)分别进行运动神经传导速度(MCV)测定，采用由近端至远端分段刺激，记录各段刺激引发的复合肌肉动作电位(CMAP)的波幅、时限、面积，并根据不同的诊断标准判定CB。...
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EMBOMolMed：基因缺陷可能指向阿尔茨海默氏病解决方案
...沉积物的增加“UiB临床医学系线粒体医学和神经遗传学（MMN）研究小组研究员JannicheTorsvik说道。从基因缺陷到大脑沉积物研究团队研究了一个由于基因缺陷而导致PITRM1大量减少的家族。该家族遭受严重的生理和心理问题影响。“...
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抑郁障碍患者五种脑诱发电位指标的临床随访研究
...电位（VEP）、事件相关电位P300（ERP-P300）、失匹性负波（MMN）和关联性负变（CNV）。对其中18例患者随访至缓解期（治疗第9周末）。结果（1）与对照组相比，患者组出现AEP-N1潜伏期长[对照组（88±18）ms，患者组（104±25）ms]，N1-P...
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肌萎缩侧索硬化症（ALS）的鉴别诊断
...电生理表现。运动神经传导阻滞对于多灶性运动神经病（mmn）、慢性炎症性多发性神经病（cidp）、急性炎症性多发性神经病（aidp）的诊断以及与als的鉴别具有重要价值。一旦发现传导阻滞或明显异常的波形离散、传导速度减慢...
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影响昏迷患者预后的多因素分析
...前应用于昏迷患者中的是ERPs中最早出现和最稳定的成分MMN(mismatchnegativity,MMN)。有些研究表明，如果昏迷患者中检测到MMN,则清醒的几率明显增高[17]。 生化指标主要包括血糖浓度、人髓鞘碱性蛋白(MyelinBasicProtein,MBP)、S100B蛋白...
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固相萃取-高效液相色谱法测定人血浆中对乙酰氨基酚
...抽千。取血样0·2m1，上固相柱，真空抽千后高真空抽滤2mmn，用蒸馏水0·4ml清洗，真空抽干后高真空抽2mmn，弃去所有滤液，换上洁净接受管，用甲醇/水(60/40)溶液0·8ml洗脱，收集洗脱液再加入内标200pl混合后，移至0·2m1聚丙烯离心...
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固相萃取-高效液相色谱法测定人血浆中对乙酰氨基酚
...抽千。取血样0·2m1，上固相柱，真空抽千后高真空抽滤2mmn，用蒸馏水0·4ml清洗，真空抽干后高真空抽2mmn，弃去所有滤液，换上洁净接受管，用甲醇/水(60/40)溶液0·8ml洗脱，收集洗脱液再加入内标200pl混合后，移至0·2m1聚丙烯离心...
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中科大生科院最新《PNAS》文章
...改变。研究人员发现，利用失匹配负波(mismatchnegativity，MMN)的整个头部电子记录，这种字调对比会引起一个关注前反应（preattentiveresponse），而且在右半球比左半球反应强烈。这就说明，如果在字调变化和一致声调之间有一个明...
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Aminoacidsasmodulatorsofendothelium-derivednitricoxide
...ensureconstantperfusion.ThekidneyswereperfusedwithKrebs-Henseleitbuffer(118mMNaCl,4.7mMKCl,2.5mMCaCl2,1.2mMMgSO4,1.2mMKH2PO4,25.0mMNaHCO3,and10mMglucose)saturatedwith95%O2-5%CO2.Thebuffercontainedphenylephrine(10-7M)tomaintainrenalperfusionpressure(RPP)at100mmHgwithaconstantperfusionrateof5ml/minusi...
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Efficacyoftwice-weeklymultiplemicronutrientsupplementationforimprovingthehemoglobinandmicronutrientstatusofanemicadolescentschoolgirlsinBang
...Agroup)ormultiplemicronutrients(15micronutrients,includingironandfolicacid;MMNgroup)for12wk.Results:Atrecruitment,thecharacteristicsofthegirlsinthe2groupswerenotsignificantlydifferent,exceptforfamilysizeandbodymassindex.Attheendofthestudy,althoughbothgroupsbenefitedsignificantlyfromsupplementation,m...
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TheimpactofDMonMHCclassII–restrictedantigenpresentationcanbealteredbymanipulationofMHC–peptidekineticstability
...osphatidyl-inositol–linkedclassIImoleculesweresolubilizedwith50mMTris,150mMNaCl,and6.5mMCHAPScontainingproteaseinhibitorsatpH7.8for1hat4°C.I-Adphosphatidyl-inositolmoleculeswerepurifiedbyclassIImAbaffinitychromatographyandtreatedwith0.1U/mlofphosphatidy-linositol–specificphospholipaseC(Sigma-Al...
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儿童阅读障碍神经学研究进展
...率和振幅均偏低，并且延长。潜伏期200ms时的负相电位（MMN）对探索LD儿童认知过程也有帮助。其依据是在纯音刺激时可以诱发出P300，但未能检测出明显的MMN。表明对音认知的障碍在早成份时即显现出异常，至晚成份出现前就有...
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TheEffectofVaryingTonicityonNasalEpithelialIonTransportinCysticFibrosis
...lutionsThestandardnasalperfusionprotocolconsistedofaphysiologicsolutionof10mMN-2-hydroxyethylpiperazine-N‘-ethanesulfonicacid(HEPES),142mMCleC,130mMNa+,6mMK+,2mMCa2+,1mMMg2+,and10mMglucoseindeionizedwater(osmolality301milliosmoles[mOsm]).Afteramiloride(100e)perfusionfor3minutes,thesolutionwassubst...
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平山病4例临床分析
...）中的肌萎缩侧索硬化和脊肌萎缩症、多灶运动神经病（MMN）等临床表现很相似,易混淆，但病因机制和预后是完全不同的,现将4例报告如下，并结合文献对其临床特征、诊断标准及影像学异常进行讨论,从而提高对本病的认识。1...
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Multiphotonimagingofrenaltissuesinvitro
...wassubsequentlycannulatedandperfusedwithamodifiedRingersolutioncontaining10mMNaCl(control)or60mMNaCltoinitiatetubuloglomerularfeedback(TGF).Thebathwascontinuouslyaeratedwith95%O2-5%CO2andexchangedatarateof1ml/min.Temperaturewaskeptat4°Cduringdissection;aftercannulationitwasmaintainedat37°C.Allanim...
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ANewMechanismof6-((2-(Dimethylamino)ethyl)amino)-3-hydroxy-7H-indeno(2,1-c)quinolin-7-oneDihydrochloride(TAS-103)ActionDiscoveredbyTargetScreening
...mMwithDMFcontaining9mMtriethylamine.TheSGNGDENCbeads(5mg)werereactedwith0.2mMN-hydroxysuccinimideand0.2mMN-ethyl-N‘-(3-dimethylaminopropyl)-carbodiimidehydrochlorideandthenmixedwith500µlofthe1.0,0.3,and0.1TAS-1-3383solutionsfor16hatroomtemperature.Afterremovalofthesupernatantstofreshtubes,50...
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MechanismoftheInsulin-ReleasingActionof-KetoisocaproateandRelated-KetoAcidAnions
...eglitinidewasperformedbytheadditionofappropriateamountsofstocksolutionsin50mMNaOH.Sodiumsaltsweredirectlydissolvedinthemedia.Theexperimentsbeganwithacontrolperiodof60-mindurationwhichwasfollowedbyatestperiodlasting44min.Theinsulincontentof1-to4-minfractionswasdeterminedbyenzyme-linkedimmunosorbentas...
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OleicAcidInhibitsAlveolarFluidReabsorption
...idsternalthoracotomy,andperfusionwasundertakenwithKrebs-Henseleitbuffer(120mMNaCl,4.3mMKCl,1.1mMKH2PO4,25mMNaHCO3,2.4mMCaCl2,1.3mMmagnesiumphosphate,0.24%[mass/vol]glucose,and5%[mass/vol]hydroxyethylamylopectin)ataflowrateof100ml/minutewithaleftatrialpressureof1.6mmHg.Lungswerefreelysuspendedfromafo...
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State-DependentVerapamilBlockoftheClonedHumanCav3.1T-TypeCa2+Channel
...nsandChemicals.Bathsolutionsforioniccurrentexperimentscontained2mMCaCl2,140mMNaCl,and10mMHEPES,andweretitratedtopH7.4with1NNaOH.ThepH7.4pipettesolutioncontained130mMNaCl,10mMHEPES,5mMMgATP,1mMCaCl2,and11mMEGTA,titratedtopH7.4with1NNaOH.ForexperimentsatpH6.1,HEPESwasreducedto5mM,EGTAwasreducedto1mM,a...
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TheplsGeneFoundinMethicillin-ResistantStaphylococcusaureusStrainsIsCommoninClinicalIsolatesofStaphylococcussciuri
...lysostaphindigestsbyusingwheatgermagglutinin(WGA)Sepharoseandelutionwith100mMN-acetylglucosamine(7)andthenanalyzedbyWesternblottingforPls(Fig.2B).PlsofS.sciuri,likethatofS.aureus,boundtoWGAandthusseemstohaveacarbohydratemoietycontainingN-acetylglucosamine.Analysisofthe“SCCmecregion“inS.sciuri.Th...
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体外循环对中性粒细胞膜结合弹性蛋白酶的影响
...：取50μl中性粒细胞稀释液，加入150μl多肽底物溶液［(1mMN-succinyl-(L-alanine）3-p-nitroanilide,200mMTris-HCl,pH=8.0,Sigma,St.Louis,USA）］，37℃下孵育60min后加入5μl冰醋酸结束反应。在405nm下比色，通过不同浓度的标准弹性蛋白酶溶液(Sigma,St.Lo...
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Theβ1SubunitofL-TypeVoltage-GatedCa2+ChannelsIndependentlyBindstoandInhibitstheGatingofLarge-ConductanceCa2+-ActivatedK+Channels
...nd<1nMfreeCa2+bufferedwith10mMEGTA,or1,5,or10µMfreeCa2+bufferedwith10mMN-hydroxy-EDTA.ThefreeCa2+concentrationsinthesesolutionswerecheckedusinganOrion97-20calciumelectrode(ThermoFisherScientific,Waltham,MA)calibratedwithcommercialsolutionstandardsobtainedfromWPI(Sarasota,FL).Currentsineachtest...
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Sexdifferencesinheatshockprotein72expressionandlocalizationinratsfollowingrenalischemia-reperfusioninjury
...homogenizedinchilledextractionbuffercontaining60mMHEPES,1mMEDTA,1mMEGTA,100mMNaCl,0.5mMphenylmethylsulfonylfluoride,0.75mg/lleupeptin,and0.1mMDTT,usingaPotterElvehjemhomogenizer.Thetotaltissuehomogenatewascentrifugedat680gfor5minat4°Ctopelletcellnucleiandlargefragments.Proteindeterminationswerep...
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SuperoxideAnionsInducetheMaturationofHumanDendriticCells
...inthephenomenon,N-nitroargininewasusedtoinhibitNOformation.Inthepresenceof1mMN-nitroarginine,theeffectsofXaXOonCD86expressionandendocytosiswereinhibitedby43.8±9.3%and64±5.2%inthreeseparateexperiments,implyingthatsuperoxideproductionmaygeneratereactiveNO-dependentmetabolites.MG-132PreventedCD86Expr...
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Theroleofinsulinreceptorsubstrate2inhypothalamicand?cellfunction
...DNAwasisolatedfrompancreaticislets,hypothalami,andothertissuesbyheatingin50mMNaOHat100°Cfor10minutes.Primerslocated1.1kbupstreamofthe5‘loxPsite(5‘-GGGAACCTGACAAGTGAATG-3‘)and0.2kbdownstreamofthe3‘loxPsite(loxP5‘-AGTCCACTTTCCTGACAAGC-3‘)amplifieda1.3-kbproductifrecombinationhadoccurred.I...
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UpregulationofPhosphodiesterase-4intheLungofAllergicRats
...llcubes.Twenty-fivemilligramsoflungtissueswerehomogenizedin100eofice-cold30mMN-2-hydroxyethylpiperazine-N‘-ethanesulfonicacid(pH7.4)containing0.1%TrionX-100(Sigma).ThecAMP-PDEassaymixture(200e)inphosphate-bufferedsaline(pH7.4)contained137mMNaCl,2.7mMKCl,8.8mMNa2HPO4,1.5mMKH2PO4,1mMCaCl2,1mMMgCl2,1...
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AngiotensinII-inducedgenomicdamageinrenalcellscanbepreventedbyangiotensinIItype1receptorblockageorradicalscavenging
...4-htreatmentwith2concentrationsofANGIIwithandwithoutcoincubationwitheither6mMN-acetylcysteine(NAC)or5µMcandesartan(Cand).Control,notreatment.SixmillimolarNACor5µMcandesartanalonehadnoeffectontheDNA(1.04±0.07and1.11±0.35%DNAintail,respectively).Thepositivecontrolmethylmethan...