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黎健教授miRNA研究连发权威期刊
...定了miR-152在调节肝糖原生成中的潜在作用。在db/db小鼠和HFD喂食小鼠的肝脏中，miR-152的表达和糖原的水平都显著下调。体内和体外研究结果表明，miR-152表达的抑制，可诱导肝细胞的糖原生成受损。有趣的是，用泵给小鼠注射16μ...
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血液净化患者肾性骨病98例临床分析
...D）治疗后β2-MG无明显变化（P＞0.05），高通量血液透析（HFD）治疗后β2-MG有明显下降（P＜0.05）。结论有效控制饮食，合理使用药物，充分纠正代谢性酸中毒、钙磷代谢紊乱,充分清除PTH、β2-MG等尿毒症毒物，可延缓肾性骨病的发...
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武汉大学Nature子刊揭示脂肪肝病因新机制
...脂肪肝的小鼠和人类肝脏中上调。在接受24周的高脂饮食(HFD)后，肝脏特异性敲除TRAF3的小鼠肥胖、胰岛素抵抗、脂肪肝和炎症反应显著减轻，而肝细胞中过表达TRAF3的转基因小鼠上述症状却加剧。他们在ob/ob小鼠中证实TRAF3对脂肪...
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ModelofInsulinResistanceandNonalcoholicSteatohepatitisinRats
...andnonalcoholicsteatohepatitis(NASH).Weusedahigh-fat,high-caloriesoliddiet(HFD)tocreateamodelofinsulinresistanceandNASHinnongeneticallymodifiedratsandtostudytherelationshipbetweenvisceraladiposetissueandliver.ObesityandinsulinresistanceoccurredinHFDrats,accompaniedbyaprogressiveincreaseinvisceraladi...
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干扰素调节因子有保护肝脏胰岛素耐受作用
...。他们发现全身IRF9敲除（KO）小鼠在长期给予高脂饮食（HFD）饲养后，更加的肥胖，胰岛素耐受、肝脂肪变性及炎症加剧。当研究人员用腺病毒在饮食诱导和遗传(ob/ob)肥胖小鼠中介导过表达肝IRF9时，证实肝胰岛素敏感性显著增...
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Effectofdifferentmacronutrientsinexcessongastricsensoryandmotorfunctionsandappetiteinnormal-weight,overweight,andobesehumans
...sof3differentmacronutrientpreloads[high-carbohydratediet(HCD),high-fatdiet(HFD),andhigh-proteindiet(HPD)]withthoseofastandarddiet(S)onthegastric-emptyinghalf-timeofsolidfoods.n=13pergroup.Medians,interquartileranges,andfullrangesareshown.Nosignificantdifferenceswereobservedacrossgroups(ANCOVA).Effec...
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LeukocyteTransglutaminase2ExpressionLimitsAtheroscleroticLesionSize
...ientforbonemarrowreconstitution,18allanimalswerefedatherogenichigh-fatdiet(HFD)(HarlanTekladdiet94059)for16weeks.18CholesterolandBloodLeukocyteCountsBloodwasobtainedbyretro-orbitalpunctureundermethoxyflurane-inducedanesthesiaaftera6-hourfastatweeksdesignated0(pre-BMT),4(4weekspost-BMT;pre-HFD),8(4we...
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AorticMsx2-WntCalcificationCascadeIsRegulatedbyTNF-–DependentSignalsinDiabeticLdlr–/–Mice
...bolicsyndromeparameters.Ldlr–/–micefedhigh-fatWesternizeddiets(HFD)accumulateaorticcalciumprimarilyinthetunicamedia,mediatedviaosteogenicmorphogensandtranscriptionalprogramsthatinduceaorticalkalinephosphatase(ALP).BecauseelevatedTNF-ischaracteristicofobesitywithT2DM,weexaminedcontributio...
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HoustonParamedicandEmergencyStrokeTreatmentandOutcomesStudy(HoPSTO)
...CommitteeforProtectionofHumanSubjectsatallsitesandtheHoustonFireDepartment(HFD)andtookplacefromSeptember1999toMarch2001.Houston,Tex,isamultiethniccityofapproximately5million.Datafromoperatingyear1998indicatedthatHFDparamedicstransportedatotalof2382suspectedstrokepatients;75%ofthesecasesweretransport...
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MolecularMechanismsofVascularCalcification
...ntsignalingisenhancedinthetunicamedia.C,LDLR-/-micefedhigh-fatWesterndiets(HFD)weretreated3daysperweekwitheithervehicle(HFD+VEH)or50ng/gmBMP2(HFD+BMP2)for4weeks.Atdeath,aorticcalciumcontentwasmeasured.BMP2significantlyincreasedaorticcalcification(Student2-sidedttest).D,Alizarinredstainingrevealedtha...
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B型超声预测胎儿体重的临床意义
...测量胎儿双顶径（BPD）、腹围（AC）、胎儿右肝前后径（HFD）和胎肾长径（KL），各项测量指标均采用目前国内外通用的测量方法［1，2］。采用目前国内外预测胎儿体重的主要公式：（1）双顶径、腹围为参数的传统预测公式f(BPD...
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色谱方法验证审评指南
...证的当前思想。需要指南的文本，请与通讯管理部联系，HFD-210，CDER，FDA，，5600FishersLane，Rockville，MD20857(电话301-594-1012)请寄一份贴好地址的标签，以帮助该办公室处理你的要求。这一指南的电子版本可通过因特网WWW获得[在WWW.fd...
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DNAVaccinationAgainstVEGFReceptor2ReducesAtherosclerosisinLDLReceptor-DeficientMice
...inalvaccinationbyfeedingthemice(n=14to16/group)aproatherogenichigh-fatdiet(HFD)containing1.25%cholesterol,15.8%fat,andnocholate(#94059,HarlanTeklad)foratotalof16weeks,afterwhichatherosclerosiswasassessed(supplementalFigureI).AllmouseexperimentswereperformedaccordingtotheNIHGuidesfortheCareandUseofLa...
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NutritionallyInducedObesityIsAttenuatedinTransgenicMiceOverexpressingPlasminogenActivatorInhibitor-1
...oteraP2andwild-type(WT)controlswerekeptonstandardfood(SFD)oronhigh-fatdiet(HFD)for15weeks.ThebodyweightandtheweightoftheisolatedsubcutaneousandgonadalfatdepositsoftheTgmicekeptontheHFDweresignificantlylowerthanthoseoftheWTmice.ThenumberofadipocytesintheadiposetissuewassimilarforTgandWTmiceontheHFD,b...
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AtheroprotectivePotentialofMacrophage-DerivedPhospholipidTransferProteininLow-DensityLipoproteinReceptor-DeficientMiceIsOvercomebyApolipoprotei
...erefedeitherachowdiet(dietNo.5015;HarlanTeklad)oranatherogenichigh-fatdiet(HFD)containing15.8%(w/w)fat,1.25%(w/w)cholesterol,andnocholate(dietNo.94059;HarlanTeklad).Bloodsampleswerecollectedfromfastedanimalsbyretro-orbitalpunctureusingheparin-coatedcapillarytubesandtransferredtoEDTA-coatedtubeskepto...
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Resistanceofmicelackingtheserum-andglucocorticoid-induciblekinaseSGK1againstsalt-sensitivehypertensioninducedbyahigh-fatdiet
...rs)andwild-typelittermates(sgk1+/+;openbars)onastandard(SD)andhigh-fatdiet(HFD)beforeandafteradditional1%NaClinthedrinkingwater.Valuesaremeans±SE;n=7/group.#P<0.05vs.respectivevalueunderanSD.P<0.05vs.respectivevaluebefore1%NaCl.*P<0.05vs.sgk1+/+.Hypoglycemiceffectofinsulinandglucosetolerancei...
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HepaticPGC-1βOverexpressionInducesCombinedHyperlipidemiaandModulatestheResponsetoPPARActivation
...tthehyperlipidemiaandgeneexpressionchangesinducedbyashort-termhigh-fatdiet(HFD)aremediatedthroughtheperoxisomeproliferator-activatedreceptorcoactivator(PGC)-1β,andthatinvitrobothPGC-1βandPGC–1increasePPAR-mediatedtranscriptionalactivities.Here,weexaminedtheinvivoeffectsofthesetwocoac...
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Inflamedfat:whatstartsthefire?
...myeloid-specificinhibitorofNF-Bkinase?(IKK?)KOanimals.Whenfedahigh-fatdiet(HFD),themacrophage-specificIKK?KOmicewererelativelyprotectedfromglucoseintoleranceandhyperinsulinemia,andeuglycemicclampstudiesshowedaglobalimprovementininsulinsensitivityinallinsulintargettissues.Thesestudiesareconsistentwit...
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Micelackingghrelinreceptorsresistthedevelopmentofdiet-inducedobesity
...tanimalsandwild-typecontrolsinbodyweightoneitherstandardchoworhigh-fatdiet(HFD).Theyalsofoundnodifferencesincumulativefoodintakeonstandardchoworbodyweightchangeandfoodintakeinresponsetore-exposuretofoodfollowingafast(12).Wortleyandcolleaguesreportedonlyslightdifferencesbetweenghrelin-knockoutanimals...
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终末期肾病高血压的发病机制与治疗现状的进展
...平。另有报道指出，血液透析（HD）、高流量血液透析（HFD）及高流量血液透析滤过（HDF）三者间对血压的影响没有差异。近来又有学者提出进行长时间血透（8h×3次/周），短时血透（2～3h×7次/周），夜间血透（夜间通宵6～7次/...
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终末期肾病高血压的发病机制与治疗现状的进展
...平。另有报道指出，血液透析（HD）、高流量血液透析（HFD）及高流量血液透析滤过（HDF）三者间对血压的影响没有差异。近来又有学者提出进行长时间血透（8h×3次/周），短时血透（2～3h×7次/周），夜间血透（夜间通宵6～7次/...
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RegulatedExpressionoftheCXCRLigandKC/GRO-inAtheroscleroticLesionsPlaysaCentralRoleinMacrophageAccumulationandLesionProgression
...-C/Cmiceand14age-matchedmaleLDLRC/C,KC/GRO-+/+controlswerefedahigh-fatdiet(HFD)thatcontained15.8%fat,1.25%cholesterol,andnocholate(dietno.94059,HarlanTeklad)for16weekstoinduceatherosclerosis.BloodwasdrawnimmediatelybeforethestartoftheHFDregimenandevery4weeksthereafter.After16weeksthemiceweresacrific...
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A4-wkhigh-fructosedietalterslipidmetabolismwithoutaffectinginsulinsensitivityorectopiclipidsinhealthyhumans
...1,2).Thisconcernissupportedbyobservationsthat,inrodents,ahigh-fructosediet(HFD)induceshepaticinsulinresistance,increasesintrahepatocellularlipids(IHCL),andstimulateshepaticdenovolipogenesiswithinafewdays(3,4).Whensustainedoverlongerperiodsoftime,highfructoseorsucroseintakesinducehepaticsteatosis(5,6...
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Absenceofghrelinprotectsagainstearly-onsetobesity
...angesinnutritionalstatus,suchasfooddeprivation(2)orexposuretoahigh-fatdiet(HFD)(4,5).Further,GHSRiscolocalizedwithneuropeptideY/agouti-relatedprotein(NPY/AgRP)neuronsinthearcuatenucleusofthehypothalamus(6),anareathatisresponsivetocirculatingnutrientsandhormonesrelatedtoenergybalance(7).Thefindingtha...
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LeptinPromotesVascularRemodelingandNeointimalGrowthinMice
...nthemousegenotypes,17someofthemicewereswitchedtoanatherogenic,high-fatdiet(HFD)2monthsbeforeinjury.Importantly,neithertheob/obnorthedb/dbmicedevelopedpronouncedarteriallesionsafterinjury,despitethepresenceofmassiveobesityandseverelyderangedglucoseandlipidmetabolismintheseanimalscomparedwiththeirWTco...
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ErythrophagocytosisbyLiverMacrophages(KupfferCells)PromotesOxidativeStressInflammationandFibrosisinaRabbitModelofSteatohepatitis
...an).Therabbitswerehousedataconstanttemperatureandsuppliedwithahigh-fatdiet(HFD)consistingofastandarddiet(SD),CR3(CLEAJapanInc.,Tokyo,Japan),supplementedwith20%cornoiland1.25%(w/w)cholesterol,adlibitumfor8weeks.ControlrabbitswerefedSD.Theamountofiron,carbohydrate,aminoacids,andothermineralswasequival...
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-Linolenicacidsupplementationforprophylaxisofatopicdermatitis—arandomizedcontrolledtrialininfantsathighfamilialrisk
...eofitchyrash(PIR;codedas1=present,0=absent),(2ahistoryofflexuraldermatitis(HFD;1=present,0=absent),(3visibleflexuraldermatitis(VFD;1=present,0=absent),and(4onsetbefore2yofage(OB2;1=present,0=absent).HFDandVFDweremodifiedtotheextensorsideofthelimbstomatchthetypicalclinicalpredilectionsitesininfants(2...
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EnhancedPIP3signalinginPOMCneuronscausesKATPchannelactivationandleadstodiet-sensitiveobesity
...hypothalamus,andadrenalglandofPPKO(blackbars)andcontrol(whitebars)femalesonHFDat12weeksofageshowedunalteredpituitaryPOMCexpressioninPPKOmice.Valuesaremean±SEM(n=6pergenotype).(D)Basalserumcorticosteronelevelsat12weeksinPPKO(blackbars)andcontrol(whitebars)HFD-fedfemales(leftpanel).Basalandstress-ind...
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RoleofGas-6inAdipogenesisandNutritionallyInducedAdiposeTissueDevelopmentinMice
...cbackground(Swiss:129SV,50:50),werefedastandardfatdiet(SFD)orahigh-fatdiet(HFD)adlibitumduring12to15weeks,asdescribed.7MicewerefromCenterforTransgeneTechnologyandGeneTherapy,FlandersInteruniversityInstituteforBiotechnology,Leuven,Belgium.Intra-abdominal(gonadal[GON])andinguinalsubcutaneous(SC)fatpad...
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GM-CSFDeficiencyReducesMacrophagePPAR-ExpressionandAggravatesAtherosclerosisinApoE-DeficientMice
...F-positivecellsduringlesiondevelopmentafterfeedingapoE-/-miceahigh-fatdiet(HFD)for4,8,and12weeks.Bargraphsrepresentnumberofcells/fieldexpressingGM-CSFindevelopinglesionsoftheaorticsinus.T0representsapoE-/-micenotonaHFD.Resultsaremeans±SEMofatleast5miceineachgroup;+P<0.05.D,Electrophoreticgels...