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海南岛黎族3个支系DYS199、Y-27H39位点多态性的研究ˇ（基金项目）
...系在2个Y-DNA基因座的遗传多态与差异。方法采用PCR扩增DYS199、Y-27H39基因座，琼脂糖凝胶电泳及变性聚丙烯酰胺凝胶电泳检测结果。结果黎族3个支系289个男性个体的DYS199位点均未发生突变，全部为C。但在Y染色体特异性微卫星Y-27H...
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IdentificationofUniqueBacterialGeneSegmentsfromStreptococcusmutanswithPotentialRelevancetoDentalCariesbySubtractionDNAHybridization
...S-ECChavingasinglegenotypeofS.mutans.AputativelyvirulentstrainofS.mutans,AF199(atesterstrain),wasisolatedfromthedentalplaqueofa7-year-oldchildfromCentralAfricawithS-ECC(decayed,missing,orfilledteeth[dmft]=14).StrainAF194(adriverstrain)wasisolatedfromacaries-freechildwholivedinthesamevillage.Twostrai...
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顶羽菊内酯Acroptilin
...aα,9bβ]st[分子式]C19H23ClO7[分子量]398.87[物理性质]熔点197～199°,(α)D+92.3°;熔点197～199°,(α)D+100°;NMR((CD32CO)δ:3.90(H-3),4.80(q,J=10.5,9,H-6),5.22(m,H-8),5.70(d,J=3.5,H-13),6.05(d,J=3.5,H-13),5.03,5.21(H-14),3.05,3.15(d,J=5,H-15),3.76,3.96(d,J=11.5,3‘-H2),1.54(s,z‘-CH...
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复方丹参注射液在兔体内的药动学研究
...（β）=0.48h，β=1.45h-1，t1/2（α）=0.07h，α=9.98h-1，AUC0→∞=199.33μg·ml-1·h-1。结论大鼠静注复方丹参注射液后，丹参素在大鼠体内呈二室开放模型，进入体内迅速分布，代谢消除也较快。 StudyofpharmacokineticsofcomplexDansheninjectioninra...
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DevelopmentofaMultiplexPCRandSHVMelting-CurveMutationDetectionSystemforDetectionofSomeSHVandCTX-M-LactamasesofEscherichiacoli,Klebsiella
...Mgenes,wereidentifiedbythesystem.ThesystemwasthenusedtogenotypetheESBLsfrom199clinicalisolates,including40Enterobactercloacae,68Escherichiacoli,and91Klebsiellapneumoniae,collectedbetweenAugust2002andMarch2003.SHV-12(80isolates)wasthemostprevalenttypeofESBLidentified,followedinorderoffrequencybyCTX-M...
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马来酸噻吗洛尔
...醚中几乎不溶。 熔点 本品的熔点（附录ⅥC）为199～203℃，熔融时同时分解。 吸收系数取本品，精密称定，加盐酸溶液(9→1000)溶解并定量稀释制成每1ml中约含20μg的溶液，照分光光度法（附录ⅣA），在295nm的波长处...
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RapidGenotypingofHepatitisCVirusbyPrimer-SpecificExtensionAnalysis
...plasma).ComparedtoINNO-LiPAHCVII,bothassaysdeterminedthesamegenotypefor191/199(96%)patientspecimens(175subtypeand16genotype-onlyidentifications).Followingtheinitialevaluation,PSEA-HCVwasusedroutinelytogenotypeHCVfrompatientspecimenssubmittedtoourlaboratory(n=312).Seventeen(5.4%)mixedinfectionswereid...
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StimulationofrenalsulfatesecretionbymetabolicacidosisrequiresNa+/H+exchangeinductionandcarbonicanhydrase
...bain,methazolamide,andEIPAwereobtainedfromSigma(St.Louis,MO).Modifiedmedium199withEarle‘ssalts(M199,Sigma)wassupplementedwith(inmM)30.0NaCl,4.2NaHCO3(airequilibrated),1.0L-glutamine,25.0HEPES,14.75NaOH(pH7.5,347mosmol/kgH2O),and20mg/ltetracycline.ModifiedM199wassupplementedwith10μg/mlinsulin,5μg...
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PersistenceofEscherichiacoliO157IsolatesonBovineFarmsinEnglandandWales
...ormedpulsed-fieldgelelectrophoresisonEscherichiacoliO157isolates(n=318)from199healthyanimalsinalongitudinalstudycarriedoutonninefarms.Investigationoftherestrictiontypesprovedthatatthefarmlevel,thesameclonescanbedetectedonsamplingoccasionsseparatedbyasmuchas17months.Thecohortanimalswererepeatedlysamp...
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RapidScreeningofUrineSpecimensforBacteriuriabytheCelleniumSystem
...tureforthedetectionofclinicallysignificantbacteriuria.Aprospectivestudyof1,199randomlycollectedurinespecimens(voidedandcatheterizedfromadultpatients)receivedbythemicrobiologylaboratoryofColumbiaUniversityMedicalCenter,wasconducted.Thespecimenswerecollectedinatransportdevice(BDVacutainer,FranklinLake...
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Zincprotectsagainstapoptosisofendothelialcellsinducedbylinoleicacidandtumornecrosisfactor
...teriesandwereculturedasdescribedpreviously(28).Cellsweresubculturedinmedium199containing10%fetalbovineserum(FBS;HyCloneLaboratoriesInc,Logan,UT)byusingstandardtechniques.Purityofthecultureswasdeterminedbyusingmorphologiccriteriaandmeasuringangiotensinconvertingenzymeactivity,orbymeasuringtheiruptake...
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SepticArthritisCausedbyVancomycin-IntermediateStaphylococcusaureus
...ki,H.,K.Kuwahara-Arai,S.Boyle-Vavra,R.S.Daum,H.Labischinski,andK.Hiramatsu.1998.Activatedcell-wallsynthesisisassociatedwithvancomycinresistanceinmethicillin-resistantStaphylococcusaureusclinicalstrainsMu3andMu50.J.Antimicrob.Chemother.42:199-209.Hiramatsu,K.2001.Vancomycin-resistantStaphylococcusaur...
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PolyunsaturatedfattyacidssuppressglycolyticandlipogenicgenesthroughtheinhibitionofChREBPnuclearproteintranslocation
...edprocedures(15).ForexperimentsusingPUFAs,themediumwaschangedtoaserum-freeM199mediumcontaining25mMglucose,100nMdexamethasone,100nMinsulin,andvariousconcentrationsofalbumin-boundstearate(C18),oleate,linoleate,EPA,orDHAatafattyacid/albuminratioof4:1for24hours.Thesourceofalbuminforallstudieswasfattyaci...
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甲胎蛋白时间分辨荧光免疫分析
...光增强液、AFPTRFIA药盒和AFP参考标准，EG－G-Wallac产品。CA199、CA125和CA153参考标准，CIBACORNING产品。AFP质量控制血清（L：21～35μg/L；M：62～93μg/L；H：173.4～260.2μg/L），中国药品生物制品鉴定所产品。全自动TRFIA检测仪（AutoDELFIA1235...
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Medicaloutcomeofpregnancyinwomenwithpsychoticdisordersandtheirinfantsinthefirstyearafterbirth
...acticeResearchDatabase.Womenwithahistoryofapsychoticdisorder,whogavebirthin1996-1998,werecomparedwithwomenmatchedforageandgeneralpractice(199casesand787controls)andtheirinfants.ResultsCaseshadahigherproportionofstillbirths(OR=4.03,95%CI1.14-4.25,P=0.03)andneonataldeaths(P0.001).Therewasnodifferencei...
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InductionofApoptosisofHumanBrainMicrovascularEndothelialCellsbyShigaToxin1
...alreddyeasdescribedelsewhere[10].Cellswereincubatedin50g/mLneutralreddyeinM199mediumwith5%fetalbovineserum(FBS)for3hat37°Candrinsedin1%formaldehydeand1%CaCl2,and50%ethanoland1%aceticacidwereadded.Absorbanceat450nmwasdetermined.Cellsgrownin24-wellplateswereanalyzedfor3H-leucineincorporationasdescrib...
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AllisnotToll:newpathwaysinDNArecognition
...ndentdendriticcellactivationbychromatin-immunoglobulinGcomplexes.J.Exp.Med.199:1631–1640.Means,T.K.,E.Latz,F.Hayashi,M.R.Murali,D.T.Golenbock,andA.D.Luster.2005.Humanlupusautoantibody-DNAcomplexesactivateDCsthroughcooperationofCD32andTLR9.J.Clin.Invest.115:407–417.Christensen,S.R.,M.Kashgarian,L...
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EpidemiologicRelationshipbetweenFluoroquinolone-ResistantSalmonellaentericaSerovarCholeraesuisStrainsIsolatedfromHumansandPigsinTaiwan(1997to
...reover,itwasthe11thmostcommonserovarcausinghumansalmonellosisinThailandfrom1993to2002(1).However,inTaiwan,SalmonellaserovarCholeraesuishasbecomeofparticularconcernsinceitwasthesecondmostcommonserovarofsalmonellasisolatedfromhumansfrom1991to1996(9,10).AntimicrobialtherapyisnecessaryforthetreatmentofS...
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TranslatingtheResultsofRandomizedTrialsintoClinicalPractice
...ofthose65to69yearsofage,59%70to79,45%80to89,and24%age90years.Oftheremaining199patients,83%had2majorriskfactorsforstroke,and98%werefelttohavecontraindicationsincludingnearly25%whowereunabletotoleratewarfarininthepast.Amongpatientsage80,fallingwasthemostoftenphysician-citedreasonfornotprescribingwarfa...
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IntensifiedandprotectiveCD4+Tcellimmunityinmicewithanti–dendriticcellHIVgagfusionantibodyvaccine
...S.,J.M.Billingsley,A.M.Caliendo,S.L.Boswell,P.Sax,S.A.Kalams,andB.D.Walker.1997.VigorousHIV-1-specificCD4+Tcellresponsesassociatedwithcontrolofviremia.Science.278:1447–1450.Martinez,V.,D.Costagliola,O.Bonduelle,N.N‘Go,A.Schruriger,I.Theodorou,J.-P.Clauvel,D.Sicard,H.Agut,P.Debre,etal.2005.Combin...
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第五节 动脉平滑肌细胞的培养
...选择：常用的有DMEM（Dulbecco'smodifiedEagles'medium），M199培养基。培养兔的平滑肌细胞用DMEM效果较好。 2．酶解离法 沿动脉纵轴切开后，刮除内皮细胞撕下中膜的内2/3，注意不要混入内皮细胞，将组织块切成1-2mm2，放...
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Continuouscontrolofautoimmunediseasebyantigen-dependentpolyclonalCD4+CD25+regulatoryTcellsintheregionallymphnode
...essionbyCD4+CD25+regulatoryTcellsinpatientswithmultiplesclerosis.J.Exp.Med.199:971–979.Ehrenstein,M.R.,J.G.Evans,A.Singh,S.Moore,G.Warnes,D.A.Isenberg,andC.Mauri.2004.CompromisedfunctionofregulatoryTcellsinrheumatoidarthritisandreversalbyanti-TNFalphatherapy.J.Exp.Med.200:277–285.Kriegel,M.A.,T....
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Blimp-1isrequiredformaintenanceoflong-livedplasmacellsinthebonemarrow
...mentofCD40.J.Clin.Invest.109:613–620.Slifka,M.K.,M.Matloubian,andR.Ahmed.1995.Bonemarrowisamajorsiteoflong-termantibodyproductionafteracuteviralinfection.J.Virol.69:1895–1902.Manz,R.A.,M.Lohning,G.Cassese,A.Thiel,andA.Radbruch.1998.Survivaloflong-livedplasmacellsisindependentofantigen.Int.Immuno...
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SmokingandElevatedBloodPressureAretheMostImportantRiskFactorsforSubarachnoidHemorrhageintheAsia-PacificRegion
...rrhage:roleofregion,year,andrateofcomputedtomography:ameta-analysis.Stroke.1996;27:625–629.FeiginVL,LawesCMM,BennettDA,AndersonCS.Strokeepidemiology:areviewofpopulation-basedstudiesofincidence,prevalence,andcase-fatalityinthelate20thcentury.LancetNeurol.2003;2:43–53.HackettML,AndersonCS.Healthou...
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CD127expressioninverselycorrelateswithFoxP3andsuppressivefunctionofhumanCD4+Tregcells
...akaguchi.2003.RegulatoryTcellsintransplantationtolerance.Nat.Rev.Immunol.3:199–210.Singh,B.,S.Read,C.Asseman,V.Malmstrom,C.Mottet,L.A.Stephens,R.Stepankova,H.Tlaskalova,andF.Powrie.2001.ControlofintestinalinflammationbyregulatoryTcells.Immunol.Rev.182:190–200.CurottodeLafaille,M.A.,andJ.J.Lafail...
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InvolvementofRhoARhoKinaseSignalinginVEGF-InducedEndothelialCellMigrationandAngiogenesisInVitro
...odsTopAbstractIntroductionMethodsResultsDiscussionReferencesMaterialsMedium199supplementedwith20mmol/LHEPES,L-glutamine,andpenicillin/streptomycinwereobtainedfromBioWhittaker;newborncalfserumwasobtainedfromGibco.Tissue-cultureplasticwarewasfromCostar.AcrudepreparationofECgrowthfactorwaspreparedfromb...
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303例老年人血压与血脂的调查分析
...BPh）;≥140/90mmHg为原发性高血压（HBP）。（2）血脂检测按1997年中华心血管病学会提出的“血脂异常防治建议”的血脂（BL）标准、总胆固醇（TC）≥6mmol/L和（或）甘油三酯（TG）≥2mmol/L为高血脂（HBL）。 2结果与分析 2.1...
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体育锻炼在肥胖治疗中的作用
...sofFat-FreeMass(%TotalWeightLoss)BallorandPoehlman.IntJObesRelatMetabDisord1994;18:35.DietPlusPhysicalActivity*P0.05EnergyExpenditureofPhysicalActivityEnergyExpenditure(kcal/h)Adaptedfrom:Alpers.UndergraduateTeachingProject.Nutrition:energyandprotein.AmericanGastroenterologicalAssociation,1978.Allou...
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射干总黄酮中的高效液相色谱/电喷雾电离-质谱分析
...alofChinapharmaceuticaluniversity(中国药科大学学报),2001,32(3):197-199. 3HuangDein(黄得林),GaoZhongyi(高仲义).WestChinaJournalofPharmaceuticalSciences.(华西药学)1997,12(2):115-116. 作者：严启新1，2，赵金华2，林永成1，刘小柔2，王存芳2
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Expressionofinterleukin(IL)-2andIL-7receptorsdiscriminatesbetweenhumanregulatoryandactivatedTcells
...CD4+CD25+regulatoryTcellsinautoimmunepolyglandularsyndrometypeII.J.Exp.Med.199:1285–1291.Crispin,J.C.,A.Martinez,andJ.Alcocer-Varela.2003.QuantificationofregulatoryTcellsinpatientswithsystemiclupuserythematosus.J.Autoimmun.21:273–276.Cao,D.,R.vanVollenhoven,L.Klareskog,C.Trollmo,andV.Malmstrom.2...