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Anextractofblack,green,andmulberryteascausesmalabsorptionofcarbohydratebutnotoftriacylglycerolinhealthyvolunteers
...SEM)breath-hydrogenconcentrationsin20subjectswhoingestedamealconsistingof50grice(carbohydrate),10gbutter,and0.2g[13C]trioleinwithateaextractbeverage()oraplacebobeverage(•),bothofwhichcontained10gsucrose.Thesignificanceofthedifferenceswasdeterminedwitha2-tailedpairedttest.Valuesobtainedwiththe2t...
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DouglasR.Green：让免疫系统去杀死肿瘤最关键
【关于DouglasR.Green】道格拉斯·格林(DouglasR.Green)博士是美国圣约翰儿童医院免疫学部主席、世界著名免疫学家。1981年DouglasR.Green在耶鲁大学获得博士学位。他所带领的研究团队主要从事细胞凋亡或主动细胞死亡的相关研究，并探...
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儿童先天性高肩胛症的手术治疗
...肩胛骨进行复位。经典的手术方法主要包括Woodward术式和Green术式。在两种手术方式的基础上，衍生出了很多改良术式。 Woodward术式是通过中线切口进行显露，松解斜方肌和菱形肌，切除肩椎骨，将肩胛骨复位。DendaneAM[3]等报...
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胸膈镜在找出淋巴结肉芽肿上比PET更可靠
...MichaelE.DeBakeyVA医疗中心与Baylor医学院的病理学教授LindaK.Green医师在一项与Medscape的访谈中说道，越来越多人依赖PET（正子摄影）作为癌症分期的工具。 Green医师表示，部份罹患肉芽肿疾病的病患在PET扫描上会有阳性结果...
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《ACSNano》：传递DNA至脑瘤细胞的可降解纳米粒子
...金斯大学医学院的生物医学工程和神经外科助理教授JordanGreen博士称：“在我们的小鼠实验中，我们设计的纳米粒子成功地将一段检测基因传递给脑肿瘤细胞，然后在那里这段基因被打开。现在我们有证据表明，这些微小粒子也...
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同性恋研究论文被指伪造数据
...。5月19日，该论文的联合作者哥伦比亚大学政治学家DonaldGreen写信要求《科学》撤销这篇2014年12月发表的论文，原因是担忧数据问题。而LaCour是这篇论文两位作者中的另一位。该研究基于一家调查公司对约9500名加州注册志愿者的...
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石榴汁的成分能抑制癌细胞迁移
...腺癌向骨的转移。加州大学里弗赛德分校(UCR)ManuelaMartins-Green博士实验室的科研人员打算在一个前列腺癌体内模型中进行进一步的测试，从而确定这两种成分的剂量依赖性效应和副作用。石榴汁对前列腺癌恶化的作用即便存在，...
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核糖体高度保守位点和蛋白合成无关
...核遗传学副教授、HowardHughesMedicalInstitute的副研究员RachelGreen博士说。“我们惊讶的发现他们似乎在合成蛋白质过程中的作用很小，而是在正常情况下加快蛋白质在适当的时候释放。”他又说，“我们发现强调了这样一个观点，即...
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Nature子刊打破常规认识，揭示过敏的触发器
...发现。西北大学Feinberg医学院病理学和毒理学教授KathleenGreen说：“Desmoglein1是一种将人类皮肤外层粘合到一起的‘胶水’。过去人们主要认为这一分子具有一种结构作用：这一细胞间的粘附物促成了一种物理屏障，调控水丧失并...
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扁平足
...，往往有明显的跟骨外翻畸形。病人尚未发育成熟，采取Grice关节外距下关节固定术，既能矫正扁平足，又不影响足的纵向发育。该手术通过外踝前方斜切口显露跗骨窦，去除跗骨窦的骨皮质后，填入自体松质骨条，并用一枚螺...
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-IsExpressedinHepaticMyofibroblastsandNotOvalCellsinStemCell-MediatedLiverRegeneration
...heImageJprogram(NationalInstitutesofHealth,Bethesda,MD).Thered(channel1)andgreen(channel2)imageswereacquiredseparatelyandsequentiallytoavoidbleed-through.Theareafraction(%)occupiedbyredandgreenfieldswasdeterminedbymanualthresholding.Analysisofco-localizedpoints(%)wasdeterminedusingtheco-localization...
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IdentificationofanovelsignalinthecytoplasmictailoftheNa+:HCO3-cotransporterNBC1thatmediatesbasolateraltargeting
...mberAF007216).Theamplifiedwild-typeNBC1DNAwasfusedtranslationallyin-frametogreenfluorescentprotein(GFP)bycloningintopcDNA3.1/NT-GFP-TOPOvector(Invitrogen,Carlsbad,CA).Site-directedmutagenesiswasperformedusingaQuikChangeSite-DirectedMutagenesisKit(Stratagene,LaJolla,CA).Thefollowingmutantsweregenerat...
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水稻中发现新型“基因开关”
...科学院院刊》（PNAS）上。这项研究是由特拉华大学CrawfordGreenewalt植物科学教授PamelaGreen以及植物和土壤科学副教授BlakeMeyers领导的，他们发现的新型分子称为自然反义微RNA（nat-miRNAs），它们是由长度大约20个核苷的短小的核糖核...
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Integrin4?1–VCAM-1–mediatedadhesionbetweenendothelialandmuralcellsisrequiredforbloodvesselmaturation
...mmunostainedtodetectexpressionoftheECmarkervWF(Figure1A,red)andintegrin4?1(green).Whiletheestablishedbloodvessels(Figure1A,arrowheads)thatarepresentinsaline-treatedtissuesdidnotexpressintegrin4?1,thoseinbFGF-orVEGF-A–stimulatedCAMtissuesdidexpressabundantintegrin4?1within24hoursaftergrowthfactorst...
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抗肝癌NMP单克隆抗体的制备及初步鉴定
...2G8 with Bel-7402 cells by LSAB（restained with methylene green）（400×） 图6 LSAB法检测单抗2G8与肝癌细胞Bel-7402反应的阴性对照（甲基绿复染）（400×） Fig 6 Reactivity of PBS with Bel-7402 cell by LSAB（restained with...
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生物钟基因影响体重增加
...icalCollegeofWisconsin）的JosephC.Besharse和弗吉尼亚大学的CarlaB.Green领导的，他们的论文发表在本周的《美国国家科学院院刊》（PNAS）上。Besharse和Green早在十多年前就发现了这种名为Nocturnin的基因，它编码的蛋白质存在于哺乳动物多...
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美国国立人类基因组研究所选出新任所长
...宣布，美国国立人类基因组研究所（NHGRI）前科学主管EricGreen将出任NHGRI新任所长。FrancisCollins为NHGRI前任所长，2008年引退。这在NIH历史上是首次由一位研究所的所长指定自己的继任者。Collins在一份声明中说：“Green是NHGRI所长的...
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甘蔗乙醇有可能成为南美国家的旗舰清洁能源资源
...能。总部设在伦敦的WhitefoxTechnologiesLtd.及其巴西合作伙伴Green与圭亚那政府签订一份乙醇合同，计划在圭亚那糖业公司一家英国清洁技术工程公司正在向乙醇生产示范工厂供应薄膜技术，帮助圭亚那挖掘其巨大的生物燃料和生物...
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TheSialomucinCDIsaMarkerofLymphaticEndothelialCellsinHumanTumors
...nsfromhumancolontumorswerestainedwithantibodiesagainstLYVE-1(red;ACI),CD31(green;ACC),andCD34(green;DCI).Alow-magnificationoverviewofCD34-positivelymphaticvesselsisshowninGCI.Intratumoraltumorlymphaticsaremarkedbyanarrow,peritumorallymphaticsaremarkedbyanarrowhead.Antibodiesweredetectedwithfluoresce...
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DifferentiallocalizationofmammalianLin-7(MALS/Veli)PDZproteinsinthekidney
...gisparticularlystrongintheglomerulus(Fig.3A),thickascendinglimb(TAL;Fig.3C,green),anddistalconvolutedtubules(DCT;Fig.3D,green).Apeptideabsorptioncontroldemonstratesthatanexcessofpeptideblockslabelingbytheanti-MALS/Veli1antibody(Fig.3B).Thelabelingpatternintheglomerulusisconsistentwithlocalizationing...
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AdipocyteDifferentiation-RelatedProteinPromotesFattyAcidStorageinCytosolicTriglyceridesandInhibitsSecretionofVeryLow-DensityLipoproteins
...cellularpooloflipiddropletswhencomparedwiththecontrol(adenovirusencodingZs-green;Figure2Aand2B).ThedifferencebetweenADRPandthecontrol(Zs-green)waslessthanthatobservedbetweeninducedanduninducedMcA-RH7777cells(Figure1Aand1B).Theexpressionefficiencymightcontributetothisdifference;whereasallMcA-RH7777ce...
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Tlxactsasaproangiogenicswitchbyregulatingextracellularassemblyoffibronectinmatricesinretinalastrocytes
...ldsinproangiogenicastrocytes.(A)Tripleimmunostaining(top)forfibronectin(FN,green),PECAM-1(red),andPDGFR(blue)anddoubleimmunostaining(middleandbottom)forlectin(red)andintegrin5or?1(green)atthesproutingvascularedgesofP5retina.(BandC)Retinalvessels24(B)or48(C)hoursafterintraocularinjectionsofcontrolIgG...
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Pathogenesisofpersistentlymphaticvesselhyperplasiainchronicairwayinflammation
...ographsoftrachealwholemountsstainedforlymphaticvessels(red)andbloodvessels(green).(A)Pathogen-freeC3Hmouse.(B)C3Hmouseinfectedfor14days;insetshowslymphaticsprouts(arrowheads)andfilopodia(arrows).(C)C3Hmouseinfectedfor28days.(D)Proliferationoflymphaticvessels(red)andbloodvessels(green)intracheasofC3H...
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MurineFLIPTransgeneExpressedonThyroidEpithelialCellsPromotesResolutionofGranulomatousExperimentalAutoimmuneThyroiditisinDBA/Mice
...gene,OR).Cytokeratin(TECs),CD3,CD4,andCD8moleculeswerevisualizedbyAlexa488(green;MolecularProbes).Apoptosiswasdetectedusinganinsitucelldeathkit(red;Roche,Nutley,NJ)withformalin-fixedparaffinsectionsofthyroidsaspreviouslydescribed.36SlideswereobservedwithaBio-RadRadiance2000confocalsystem(Bio-Rad,Her...
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DetectionandCharacterizationofHumanGroupCRotavirusesinBangladesh
...groupBandCrotavirusesbypolymerasechainreaction.J.Clin.Microbiol.29:519-523.Grice,A.S.,P.R.Lambden,E.O.Caul,andI.N.Clarke.1994.SequenceconservationofthemajoroutercapsidglycoproteinofhumangroupCrotaviruses.J.Med.Virol.44:166-171.Herring,A.J.,N.F.Inglis,C.K.Ojeh,D.R.Snodgrass,andJ.D.Menzies.1982.Rapidd...
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MyeloidCellsinInfantileHemangioma
...blue)toidentifyhematopoieticcells,ulexlectin(red)toshowendothelium,andKi67(green)toshowdividingcells.Theseresultsdemonstratethatcellularproliferationisprimarilyrestrictedtohemangiomaendothelium.b:Inparallel,aninvolutingcasewaslabeledshowingsignificantreductioninthenumberofCD45+cellsandKi67staining,i...
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ThecontributionofplantfoodstothevitaminAsupplyoflactatingwomeninVietnam:arandomizedcontrolledtrial
...okskeptarecordofattendanceandrecordedthefoodsleftoveraftereachmeal,excludingrice.ThewomenwereaskedwhattheyateforbreakfasteachdayandfortheirmealsonSunday.MeasurementsOnthedaybeforethefirstmealandonthedayafterthelastmeal(10wkafterstartoftheintervention),womenwereexaminedmedically,avenousbloodsamplewas...
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最新Cell公布功能基因网络研究新技术
...圣地亚哥分校医学院Ludwig研究所的博士后工作人员RebeccaGreen决定采用一种不同的方法。“我们没有对单个细胞进行监控，而是在多细胞生物体的复杂组织结构（此处是线虫的生殖器官）上研究了基因的抑制效应，”Green说。科学...
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CDIdentifiesPerivascularMacrophagesinNormalandViralEncephaliticBrainsandPotentialPrecursorstoPerivascularMacrophagesinBlood
...ctcolorsofuptofourchannelscollected,includingthefluorochromesAlexaFluor488(green),AlexaFluor568(red),andAlexaFluor633(blue)andthedifferentialinterferencecontrastimage(grayscale).SomeimageswererenderedwithVolocity3.6Software(Improvision,Lexington,MA).Colocalizationofantigensisindicatedbytheadditionof...
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PreferentialAttachmentofPeritonealTumorMetastasestoOmentalImmuneAggregatesandPossibleRoleofaUniqueVascularMicroenvironmentinMetastaticSurv
...D),accordingtothemanufacturer??sdirections.Cellsexpressingthehighestlevelofgreenfluorescentprotein(GFP),asdeterminedbyflowcytometry,wereselectedbysubcloning.WholeMountAnalysisandFluorescenceMicroscopyPrimaryandsecondaryantibodiesusedforwholemountimmunocytochemistryarelistedinTable1.Wholemounthistolo...