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第二节 无芽胞厌氧菌
...苹果酸盐脱氢酶、谷氨酸脱氢酶、葡萄糖6磷酸脱氢酶（G6PDH）、6磷酸葡萄糖脱氢酶（6PGDH）、细胞壁粘肽层含有二氨基庚二酸。依据对鼠李糖、海澡糖，甘露醇发酵及吲哚试验可区别本组菌落。DNA的G+C含量为39～48%。本菌主要分...
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Renaloxidativevulnerabilityduetochangesinmitochondrial-glutathioneandenergyhomeostasisinaratmodelofcalciumoxalateurolithiasis
...(GPx)andglutathionereductase(GR),whereasglucose-6-phosphatedehydrogenase(G6PDH)suppliesreducingequivalentsintheformofNADPH,akeycofactorinmaintainingglutathioneinitsreducedform.OxalateexposureofrenalcellsresultsinpathologicalchangesinvolvingreinitiationofDNAsynthesis,cellgrowth,cellinjury,anddeath(16...
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Survivin基因在大肠癌中的表达
...mRNA提取和逆转录效应的可信性，以3磷酸甘油醛脱氢酶(G3PDH)表达作为内参照，其PCR条件与下述所描述的条件一致。Survivin基因引物序列：正向引物5’-GGAC-CACCGCATCTCTACAT-3’(位于外显子-1的2855-2984);反向引物5’-GCACTTTGCCAGTTTCC...
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大鼠肝微粒体温孵体系中
...泰生化试剂公司，G-6-P(6-磷酸葡萄糖)，G-6-Pdehydrogenase(G-6-PDH,G-6-P脱氢酶)为Sigma公司产品，苯巴比妥钠(PB，药用，进口分装)，乙腈为色谱纯，其他化学品均为市售分析纯，水为蒸馏水经MilliQLabo纯水器处理。(250±10)g雄性纯种Wistar大...
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第二节 氯霉素
...酶原时间，以防药效及毒性增强。3.对肝肾功能不良，G-6PDH缺陷者、婴儿、孕妇、乳妇应慎用。4.用药时间不宜过长，一般不超过二个月，能达到防止感染复发即可，避免重复疗程。 制剂与用法 盐酸四环素（tetracycline hy...
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ReducedmitochondrialdensityandincreasedIRS-1serinephosphorylationinmuscleofinsulin-resistantoffspringoftype2diabeticparents
...hondrialgenome,wasdecreasedby50%inIRoffspring(P0.05).Pyruvatedehydrogenase(PDH)andsuccinatedehydrogenase(SDH),whicharebothencodedbythenucleargenome,tendedtobedecreasedinIRoffspring(Figure3B).Figure3Mitochondrialdensityandgeneexpressiondata.(A)Mitochondrialdensityincontrolsubjects(n=6)andIRoffspring(...
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第二节 抗麻风病药
...良反应】较常见为贫血，偶可引起急性溶血性贫血，G-6-PDH缺乏者尤易发生。有时出现胃肠刺激症状、头痛、失眠、中毒性精神病及过敏反应。剂量过大还可引起肝损害及剥脱性皮炎。治疗早期或增量过快，患者可发生麻风症状...
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阿替洛尔的手性衍生化RP-HPLC法拆分及在鼠肝微粒体中的测定
...(NADPH)、GITC、6-磷酸葡萄糖(G-6-P)、6-磷酸葡萄糖脱氢酶(G-6-PDH)均购自美国Sigma公司，阿替洛尔购自中国药品生物制品检定所。 实验中所用试剂均为分析纯试剂。 NaH2PO4缓冲液：精密称取NaH2PO4.2H2O0.7801g，精确加入水500.0mL，混...
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肿瘤坏死因子α、氧化型低密度脂蛋白对人单核细胞株U937胆固醇酰基转移酶mRNA表达的影响
...共25个循环后75℃延伸10min。同时取2μlcDNA-RNA模板，加入G3PDH引物（终浓度为1μmol/L）:5′-TCCCTCAAGATTCTCAGCAA-3′，5′-AGATCCACAACGGATACATT-3′。PCR条件为:94℃变性1min，56℃退火1min，72℃延伸1min，共35个循环。最后72℃延伸7min。扩增后进行3...
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神经营养因子家族及其受体在重组人骨形态发生蛋白2诱导成骨中的表达
...kara一步法RTPCR试剂盒扩增小鼠NGF、BDNF、NT3及内参照G3PDH的cDNA。经15％琼脂糖凝胶电泳后，采用凝胶图像系统进行图像采集和分析。根据小鼠上述cDNA序列设计引物：NGF上游引物：5′ATAAAGGTTTTGCCAAGGACG3′，下游引物：5′...
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Hypoxia-inducedneutrophilsurvivalismediatedbyHIF-1–dependentNF-Bactivity
...ver,withamoreprolongedculture(6h),glyceraldehyde3-phosphatedehydrogenase(G3PDH),MIF,triosephosphateisomerse-1,andNF-B(p65)showedatwofoldormorerelativeincreaseinexpressioninbothhypoxiaandanoxiacomparedwithnormoxia(Fig.6A).ThiswasshowntobesignificantbyBayesianttests(Fig.6A,table).Wesubsequentlyconfirm...
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地蒽酚疗法的进展
...活性物质[11]，它能在短期内抑制6-磷酸葡萄糖脱氢酶（G-6PDH）的活性，通过照射紫外线，能促进这种高生物活性物质的形成。同时，日光、分子氧、焦油、碱溶液等也会促进地蒽酚氧化。因此，经典的联合疗法就是加照UVB，或加...
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Reperfusion-InducedTranslocationofPKCtoCardiacMitochondriaPreventsPyruvateDehydrogenaseReactivation
...sociatedwithlossintheactivityofthemitochondrialenzymepyruvatedehydrogenase(PDH).PharmacologicalstimulationofPDHactivityimprovesrecoveryincontractilefunctionduringreperfusion.SignalingmechanismsthatcontrolinhibitionandreactivationofPDHduringreperfusionwerethereforeinvestigated.Usinganisolatedratheart...
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毛细血管血血糖和静脉血浆血糖相关性分析
...肘静脉血，用奥林巴斯AU640生化分析仪,己糖激酶（G6PDH）方法测定空腹肘静脉血浆血糖。 1.3质量控制方法由专人负责血样的离心、冻存及血糖检测，为了控制质量，课题组按5％比例进行抽样复查，结果的一致率为95％。1...
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摩托罗拉“药丸”的背后：身体密码让人欢喜让人忧
...管理局（FDA）就曾通过了一款由普罗透斯数字健康协会（PDH）发明的数字药丸，以确保患者及时使用他们的药丸。 PDH开发的硅芯片，小如一粒细沙。人体通过与消化液互动，可激发药丸内的铜和镁而产生电压，以激发其发挥...
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小睑裂综合征FOXL2基因突变分析
...放性阅读框及5’非翻译区，产物直接或克隆后测序。用PDH和ExPASy软件包对正常及发生突变后的蛋白质结构进行预测，分析比较它们一、二级结构的差异。对小睑裂综合征（BPES）患者进行FOXL2基因突变筛选，并对基因突变前后的...
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ThromboxaneA2PromotesInterleukin-6BiosynthesisMediatedbyanActivationofCyclicAMP-ResponseElement-BindingProteinin1321N1HumanAstrocytomaCells
...or30s,57°Cfor60s,and72°Cfor60s).Glyceraldehyde3-phosphatedehydrogenase(G3PDH)transcriptsof700basepairswereusedasaninternalcontrol.Thenumberofcyclesthatyieldedaquantitativeamountofproductwasdeterminedinpreliminaryexperiments.ThedensitiesofthebandscorrespondingtoIL-6andG3PDHwereanalyzedbydensitometr...
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第二节 糖的分解代谢
...在脊椎动物还有第二层次的调节，即酶蛋白的化学修饰，PDH含有两个亚基，其中一个亚基上特定的一个丝氨酸残基经磷酸化后，酶活性就受抑制，脱磷酸化活性就恢复，磷酸化-脱磷酸化作用是由特异的磷酸激酶和磷酸蛋白磷酸...
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Emerin-LackingMiceShowMinimalMotorandCardiacDysfunctionswithNuclear-AssociatedVacuoles
...TGATGGAAGGTATCAGCATCTACA-3‘)andglyceraldehyde-3-phosphatedehydrogenase(G3PDH)(forward:5‘-CTGGAGAAACCTGCCAAGTATG-3‘,reverse:5‘-TTGAAGTCGCAGGAGACAACCTG-3‘)wereused.ThevaluesofmRNAforemerinwerenormalizedtothatofG3PDH.ClinicalExaminationsEachmousewasweighedevery2weeksfrom3weeksofage.Musclestre...
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流感加重或源于特定激酶“捣乱”有望研发防治新药
...著增多。PDK4会使实验鼠体内与能量生产相关的另一种酶PDH的作用减弱。其结果是细胞内能量枯竭，抵抗力下降，进而导致肺炎、多脏器衰竭等。研究人员对患重症流感的40只实验鼠使用可抑制PDK4作用的药物，这些实验鼠两周后...
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IncreasedCollagenDepositionandDiastolicDysfunctionbutPreservedMyocardialHypertrophyAfterPressureOverloadinMiceLackingPKC
...):5‘-ATCTCACGTTCAGCTGTGGTCA-3‘;5‘-ACCACCGGCATCG-TGTTGGAT-3‘;22andG3PDH:5‘-AACGACCCCTTCATTGAC-3;5‘-TCCACGACATACTCAGCAC-3.PCRwasperformedwithdenaturationat95°Cfor1minute,annealingat57°Cfor1minute,andelongationat72°Cfor30seconds(40cycles).Dataacquisitionwasaccomplishedat80°Ctoavoidmeasu...
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Ornithinemetabolisminmaleandfemaleratkidney:mitochondrialexpressionofornithineaminotransferaseandarginaseII
...1:2,000);andamonoclonalmouseanti-glyceraldehyde-3-phosphatedehydrogenase(G3PDH;dilution1:170).Theblotswerewashedthreetimesfor20minin1xTBSTandincubatedfor60minwitheitherperoxidase-conjugatedanti-rabbitIgGoranti-mouseIgGsecondaryantibodies(dilution1:10,000)in5%milk-1xTBST.Blotswerewashedthreetimesfor1...
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PolyunsaturatedfattyacidssuppressglycolyticandlipogenicgenesthroughtheinhibitionofChREBPnuclearproteintranslocation
...ate-limitingenzymeofglycolysis)andofglucose6-phosphate(G6P)dehydrogenase(G6PDH;therate-limitingenzymeofthepentosephosphatepathway)requiredforthesynthesisofX5PfromG6P.RefeedingmicewithaHCHOdietfor18hoursorculturinghepatocytesinthepresenceof25mMglucoseand100nMinsulinfor24hoursledtoaparallelincreaseinG...
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DNAMethyltransferaseInhibitorsCoordinatelyInduceExpressionoftheHumanReelinandGlutamicAcidDecarboxylase67Genes
...isolatedafterultracentrifugationthroughCsCl(Chenetal.,2002).Reelin,GAD67,G3PDH,andDNMT1mRNAcontentsweremeasuredbycompetitiveRT-PCRwithinternalstandardsasdescribedpreviously(GraysonandIkonomovic,1999).Primersweredesignedtominimallycrossatleastoneexon/intronboundary.Forexample,the5‘DNMT1primerreside...
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特定基因或蛋白质的数据库
...ontainingsystems IARCp53db-IARCdbofsomaticp53mutations PDZdomain-PDH/DHRdomainWWWpage PDE-Phosphodiesterases Peroxidases PlantPeroxidases PeptidasesinSWISS-PROT-NomenclatureandindexofpeptidasesinSWISS-PROT MEROPS-Peptidasedb Prolysis-Proteasesandinhibitorsserv...
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高中生听力损失1924例流行病学调查
...5、1、2、3、4、6、8kHz7个频率[1]。 1.4诊断标准根据WHO/PDH97.3推荐标准，以0.25之4kHz平均听阈对受试者进行听力损失程度的分级：轻度26～40dBHL，中度41～60dBHL，重度61～80dBHL，极重度≥81dBHL[2]。 1.5统计学方法用SPSS13.0软件进...
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MitochondrialNADP+-DependentIsocitrateDehydrogenaseProtectsCadmium-InducedApoptosis
...eNADPHpoolandGSHrecycling(Leeetal.,2003).Glucose6-phosphatedehydrogenase(G6PDH)isimportantintheproductionofNADPHforGSHrecyclingincytosol.IthasbeenshownthatalowerconcentrationofCd2+treatmentincreasestheexpressionofG6PDHasmeasuredbymRNAlevelsandenzymeactivityinrathepatocytes(Xuetal.,2003).Furthermore,...
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Inflammationandthereciprocalproductionofgranulocytesandlymphocytesinbonemarrow
...cientinCXCL12.mRNAspecificforBcell–activatingfactor(BAFF),CXCL2,CXCL12,GAPDH,GM-CSF,hypoxanthine-guaninephosphoribosyltransferase(HPRT),IL-1,IL-3,IL-7,andSCFwerequantifiedrelativetoGAPDHmessageatvarioustimesafterimmunization(TableIandFig.3A).Asexpected,CXCL12messagedecreased10-foldatdays4and8after...
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HypochlorousAcid,aMacrophageProduct,InducesEndothelialApoptosisandTissueFactorExpression
...AAGCAGTGA-TTCCCTCTCG-3‘;antisense,5‘-AACACAGCATTGGCAGCAG-3‘andhumanG3PDH:sense,5‘-GGAGCCAAA-AGGGTCATC-3‘;antisense,5‘-CCAGTGAGTTTCCCGTTC-3‘.WeconductedPCRfor25cycles(confirmedtobewithinthelog-linearrangeofamplification)forTFandG3PDH,andtheproductswereelectrophoresedthrougha2.0%agarose-...
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绿激光前列腺汽化(PVP)治疗前列腺癌导致的下尿路梗阻降低前列腺癌播散危险性的临床研究
...12]。这种设计保证了PSA反转录PCR的产品是来自PSA的mRNA。GAPDH的引物序列为：顺向引物CGACCACTTTGTCAAGCTCA，逆向引物AGGGGAGATTCAGTGTGGTG。④PCR反应条件：PCR反应用了5μL的cDNA。反应总量为25μL，包括2.5μLPCR缓冲液(Invitrogen,英国)，0.75μLMgCl2...