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与维生素B12相关的酶
...关性研究，寻找可能影响B12水平的普通基因变异。在基因FUT2中，他们发现三种高度相关的单核苷酸多态性SNPs与B12的水平有关。FUT2负责编码一种与某种糖类相关的酶，这种糖参与调节了构成个体血型的某种因子的分泌。其中一个...
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Molecularcharacterizationofthemercurialsensitivityofafrogureatransporter(fUT)
【摘要】Theamphibianureatransporter(fUT)sharesmanypropertieswiththemammalianureatransporters(UT)derivedfromUT-AandUT-Bgenes.ThetransportofureabyfUTisinhibitedbythemercurialagentp-chloromercuribenzenesulfonicacid(pCMBS).WefoundthatinoocytesexpressingcRNAencodingfUT,a5-minpreincubationin0.5mMmercu...
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中国人群血型基因研究取得成果
...发现了10个新的ABO血型等位基因，两个Dombrock基因，两个FUT2基因和1个FUT1新等位基因。其中，有11个等位基因在国际上是第一次被发现，并已被国际基因库、国际血型抗原基因突变数据库收集和认可。该研究还建立了中国人群相关...
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安徽医科大学NatureGenetics新文章
...常见错义突变，其关联达到了全基因组显著水平。并且在FUT2和TARBP1基因中发现了一些罕见的错义SNVs,证据表明它们也与银屑病相关。除了SNVs分析，研究人员还对622个免疫相关基因进行了调查，结果显示编码变异，至少常见和低...
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AssociationofHistoBloodGroupAntigensandSusceptibilitytoNorovirusInfections
...aintypesofprecursorsaccharidestructures.Twoenzymes(1,2-fucosyltransferases[FUT1andFUT2])areresponsibleforsynthesisofHantigenfromtheprecursorchainbyadditionofafucosegroup.TheHantigenthenservesasthesubstrateforAandBglycosyltransferases,whichaddtheAorBepitopesthatdeterminetheABOhistobloodgroupphenotype...
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浙江省经济欠发达地区小学生营养KAP状况调查
...SULLIVANKR.Changingpatternsofunderandover-nutritioninSouthAfricanchildren-futurerisksofnon-communi-cablediseases.AnnalsofTropicalPaediatrics，2005，25（1）:3-15. ［2］LYTLELA.Nutritioneducation，behavioraltheories，andthescientificmethod:Anotherviewpoint.JNutritEdu&Behav，2005，37（2...
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第一节 角朊细胞
...的抑制剂如α2-巨球蛋白、STBI以及合成抑制剂Foy、Foy-305、FUT-175、止血芳酸等均能在一定程度上抑制Pab导致棘层松解的产生。 4．天疱疮样抗体与血型抗体 Ahmed及Workman检查1500份正常血清，发现14例有低滴度的抗表皮细胞间...
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DietarypatternsrelatedtoglycemicindexandloadandriskofpremenopausalandpostmenopausalbreastcancerintheWesternNewYorkExposureandBreastCan
...IandGLinestimatingtheassociationofthedietaryglycemiceffectwithbreastcancer.Futurestudiesarewarrantedtoincorporatebiomarkersofglycemiccontrol,suchasinsulin,C-peptide,andHbA1c,tofurtherimprovetheidentificationofrelatedfoodsandfoodgroups.Moretargetedidentificationofspecificdietarypatternscouldhelpinour...
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蓖麻毒素毒性作用机理研究进展
...edtoxiclectinsoneukaryoticribosomes.JBiollChem,1987,262：5908. [4] FuT,BurbageC,TaggeEp,etal.Ricintoxincontainsthreelectinsiteswhichcontributetoitsinvivotoxicity.IntJImmunopharmacol,1996,18：685-692. [5] TraceyKJ,BeutlerB,LowrySF,etal.Shockandtissueinjuryinducedbyrecombinanthumancach...
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TAP与急性胰腺炎
...地激活酶原，使TAP显著升高。如预先给予胰蛋白酶抑制剂Fut-175，则明显减少TAP的释放，而组织蛋白酶B抑制剂E-64和NCO-700则无此作用。他认为胰蛋白酶原自动活化是酶原活化的主要机制。Steel等[13]认为可能E-64的细胞渗透力不强，...
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ProgressionofAirwayDysplasiaandC-ReactiveProteininSmokersatHighRiskofLungCancer
...alswithprecancerousbronchiallesionsandwhetherthesebloodbiomarkerscanpredictfutureprogressionofbronchialdysplasiaareunknown.Iftheserelationshipsexist,thesedatawouldbeusefulindesigningfuturechemopreventionandearlydetectiontrialsbecausebloodbiomarkersarerelativelynoninvasiveandeasilyobtainableinaclinic...
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蛋白质组学在心脏疾病研究中的应用
...疾病有关［15］。 SchwertzH等用人工合成丝氨酸抑制物FUT-175处理心肌局部缺血兔子模型，处理后心肌局部缺血得到缓解。对处理前后心脏蛋白质组进行研究，有10个蛋白点发生改变，其中超氧化物歧化酶前体和αB-晶状体蛋白下...
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HemodynamicShearStressesinMouseAortas
...amentalresponsesofMAECsandhumanendothelialcellsinresponsetoWSSmaybeafactor.FutureworkisneededtoinvestigateingreaterdetailtherelationshipbetweenlowandoscillatingWSSandothermarkersassociatedwithatherogenesisinmiceusingthismethodology.Additionally,ourimagingtechniquesmaybeimproved,forexample,byusingmic...
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SerumAmyloidPandCardiovascularDiseaseinOlderMenandWomen
...nareselectivelyboundbyaggregatedC-reactiveprotein.JExpMed.1982;156:230-242.FuT,BorensztajnJ.Macrophageuptakeoflow-densitylipoproteinboundtoaggregatedC-reactiveprotein:possiblemechanismoffoam-cellformationinatheroscleroticlesions.BiochemJ.2002;366:195-201.StewartCR,TsengAA,MokYF,StaplesMK,SchiesserCH...
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Ureamayregulateureatransporterproteinabundanceduringosmoticdiuresis
...ine-concentratingability.Totestourhypothesis,wemeasuredtheproteinabundanceofUT-A1duringseveralmodelsofosmoticdiuresisinwhichthepercentageofureaintotalurinarysolutewasvaried.WealsomeasuredtheabundancesofUT-A2,UT-B,andNKCC2/BSC1toevaluatethespecificityofanychangesinUT-A1.METHODSAnimalpreparation.Allan...
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治疗性工程抗体的研究进展
...IgG糖基化关键酶－盐藻糖基转移酶8（a1,6fucosyltransferase，FUT8）的表达，使抗体去盐藻糖化，结果该抗体的ADCC效应增强了100倍，该CHO的表达特性还可以遗传至子代细胞。此外，完整的人源IgG1与FcγR结合的氨基酸序列图谱的成功解...
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ProtectionagainstHumanImmunodeficiencyVirusTypeTatNeurotoxicitybyGinkgobilobaExtractEGbInvolvingGlialFibrillaryAcidicProtein
...linhibitsP-TEFbandblocksHIV-1replication.JBiolChem2000,275:28345-28348LiBQ,FuT,DongyanY,MikovitsJA,RuscettiFW,WangJM:FlavonoidbaicalininhibitsHIV-1infectionatthelevelofviralentry.BiochemBiophysResCommun2000,276:534-538EngLF:Glialfibrillaryacidicprotein(GFAP):themajorproteinofglialintermediatefilamen...
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C-ReactiveProteinandAnnexinA5BindtoDistinctSitesofNegativelyChargedPhospholipidsPresentinOxidizedLow-DensityLipoprotein
...ormalrange(1to10mg/L)measuredbyhighlysensitiveassays,isastrongriskfactorforfuturecardiovascularevents.1,2Thisreflectstheconceptofatherosclerosisasalow-gradeinflammatorystate.IthasbeendemonstratedthatCRPcanbesynthesizedbymacrophagesandsmoothmuscle–likecellspresentinhumanatheroscleroticplaques.3,4Th...
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MacrophageApolipoproteinEReducesAtherosclerosisandPreventsPrematureDeathinApolipoproteinEandScavengerReceptor-ClassBIDouble-KnockoutMice
...stersinapolipoproteinEknock-outmice.ProcNatlAcadSciUSA.1999;96:12050-12055.FuT,KozarskyKF,BorensztajnJ.OverexpressionofSR-BIbyadenoviralvectorreversesthefibrateinducedhypercholesterolemiaofapolipoproteinE-deficientmice.JBiolChem.2003;278:52559-52563.OutR,KruijtJK,RensenPC,HildebrandRB,deVosP,VanEckM...
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CD4+CD25+RegulatoryTLymphocytesinMalignantPleuralEffusion
...bythetranscriptionfactorFoxp3.Science2003;299:1057–1061.VooKS,PengG,GuoZ,FuT,LiY,FrappierL,WangRF.FunctionalcharacterizationofEBV-encodednuclearantigen1-specificCD4+helperandregulatoryTcellselicitedbyinvitropeptidestimulation.CancerRes2005;65:1577–1586.ChenL,AsheS,BradyWA,HellstromI,HellstromKE,...
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ComplementC3aandC4aIncreasedinPlasmaofPatientswithAspirin-inducedAsthma
...4weremeasuredbyimmunoturbidmetricassays(RocheDiagnostics,Mannheim,Germany).FUT-175(BDBiosciences,PaloAlto,CA)wasaddedasanadditivetostabilizeplasmasamplesforcomplementmeasurements(finalconcentrationof50μg/mlplasma).TheconcentrationsofC3aandC4aweredeterminedbyenzyme-linkedimmunosorbentassays(AssayDes...
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Anoveltypeofureatransporter,UT-C,ishighlyexpressedinproximaltubuleofseawatereelkidney
...ndcomparison.ThededucedaminoacidsequenceofeUT-CwascomparedwiththesequencesofUTfamilymembersofotherspecies.ProteinsequencesofmammalianUTfamiliesandotherfishUTswereobtainedfromtheGenBanksequencedatabase.ProteinsequencesofUTfamilymembersofthefugu,zebrafish,andrainbowtroutwerededucedfromnucleotidesequen...
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FenofibrateReducesAtherogenesisinApoE3LeidenMice
...rinogen.Theseliver-derivedmarkershavebeendemonstratedtoindependentlypredictfuturecardiovascularrisk,8,28andCRPreductionaftercholesterol-loweringtherapyisassociatedwithimprovedclinicaloutcomes.29PatientstreatedwithfenofibrateatadosecomparabletothedoseusedinthisstudydisplayreducedplasmalevelsofCRP,SAA...
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Ureaandurineconcentratingability:newinsightsfromstudiesinmice
...iewdescribes1)therenalhandlingofureabythemammaliankidney;2)theconsequencesofUT-Bdeletiononurinaryconcentratingability;and3)speciesdifferencesamongmice,rats,andhumansrelatedtotheirverydifferentbodysizeandmetabolicrate,leadingtoconsiderablylargerneedstoexcreteandtoconcentrateureainsmallerspecies(ureae...
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Cytokine-mediatedregulationofureatransportersduringexperimentalendotoxemia
...vasopressin-dependentincreaseintheureapermeabilityoftheterminalIMCDbecauseofUT-A1,UT-A3,andUT-A4enablestransportationoftheconcentratedureaintheinterstitialtissueofthedeepIM;and3)medullaryurea,whichescapestheIMviatheascendingvenousvasarecta,iscontinuouslyreturnedtotheIMbyacomplexintrarenalurearecycli...
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ThebasolateralexpressionofmUT-A3inthemousekidney
...22),whereasafifth,UT-A5,isexpressedinthetestis(7).AlthoughthepotentialroleofUT-Aureatransportersintheurinaryconcentratingmechanismisestablished,theprecisefunctionofsomeoftherenalUT-Aisoformsremainstobeclarified.Intherat,UT-A1ispresentexclusivelyintheinnermedullarycollectingduct(IMCD)(14,20).Itisloca...
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Developmentofwatertransportinthecollectingduct
...10wkofpostnatallifeusinganantibodyagainstUTA-1.WeobservedthattheexpressionofUTA-1isdevelopmentallyregulated,withlowerlevelsobservedduringthefirst2wkoflife(Fig.8)(10).Kimandcolleagues(61)havereportedsimilarresults.Fig.8.ImmunoblotanalysisofureatransporterUTA-1postnataldevelopmentalexpressioninratsbet...
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p53andATM/ATRRegulate7,12-Dimethylbenz[a]anthracene-InducedImmunosuppression
...3-independentpathwaysthatareassociatedwithspleencellcytotoxicity.Therefore,futurestudieswillcontinuetoinvestigatetheroleofATM/ATRandp53activationintheimmunotoxicityofotherPAHfamilymembersandotherchemicalagents.ManychemicalxenobioticsthatinducegenotoxicitymayactivateATMandATR,leadingtop53up-regulatio...
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ProstateCancer-AssociatedMembraneType-MatrixMetalloproteinase
...SinghRK,Vargo-GogolaTC,BegtrupJL,PetersonTE,FingletonB,ShiraiT,MatrisianLM,FutakuchiM:MMP-7promotesprostatecancer-inducedosteolysisviathesolubilizationofRANKL.CancerCell2005,7:485-496BrownJM,CoreyE,LeeZD,TrueLD,YunTJ,TondraviM,VessellaRL:Osteoprotegerinandrankligandexpressioninprostatecancer.Urology...
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DualPotentiatingandInhibitoryActionsofaBenz[e]indeneNeurosteroidAnalogonRecombinant122GABAAReceptors
...actionthanthecorresponding3-hydroxysteroidsareatdiminishingGABAaction.Inthefuture,itwillbeinterestingtodeterminewhethersulfationofthehydroxylgroupofBI-2oranotherbenz[e]indenewillresultincompoundsdevoidofpotentiationactionbutwithmorepotentinhibitoryaction,asgenerallyhasbeenfoundforsulfatedsteroidsact...