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经内镜胆道支架置入术的疗效观察与护理
...行内镜胆道塑料支架内引流术(ERBD)和金属支架内引流术(EMBE)，进行内镜下治疗的配合和护理。结果：成功实施ERBD86例，EMBE34例，引流1～2周平均血清胆红素下降209mmol/L。结论：ERBD和EMBE是治疗梗阻性黄疸的有效方法。充分的术前...
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Opticnerveoxygentension:theeffectsoftimololanddorzolamide
...edwithapolarographicoxygenelectrodewithaninternalAg/AgClreferenceelectrode,embeddedina20gaugeneedle(model768–20R,DiamondGeneralDevelopmentCorporation,AnnArbor,MI,USA).Theelectrodewascalibratedandtheopticnerveoxygentensionmeasumentwereperformedaspreviouslydescribed.12ExperimentalprotocolFrequentart...
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Roleofhemeoxygenase-1inendotoxemicacuterenalfailure
...tionofHO-1.Tissuesampleswerefixedin4%formaldehydesolutionfor24h,dehydrated,embeddedinparaffin,andcutinto3-μm-thickslices.Immunohistochemicalstainingwasperformedusingtheavidin-biotinylatedperoxidasemethod.Afterdeparaffinizationandrehydration,sectionswerepretreatedwith0.3%hydrogenperoxidasein70%metha...
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EvidenceofParvovirusReplicationinCerebralNeuronsofCats
...institut,TiergesundheitsdienstBayerne.V.,D-85586Poing,Germany2Received27November2002/Returnedformodification12January2003/Accepted26May2003 ABSTRACTTopAbstractIntroductionMaterialsandMethodsResultsDiscussionReferencesThecorrelationbetweenparvovirusinfectionsandlesionsinthecentralnervoussystemother...
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Five-YearFollow-UpStudyofMother-to-ChildTransmissionofHelicobacterpyloriInfectionDetectedbyaRandomAmplifiedPolymorphicDNAFingerprintingMetho
...1year6months,1year8moand4years4months,respectively(Table1).Case3a,borninDecember1998,andcase3b,borninFebruary2000,weresiblings.Duringthefollow-upperiod,anothertwochildrentestedpositiveforHpSAat3years5monthsand3years7months.Sincenoisolateswereharvestedfromtheirgastricjuicesamplesandbothtestednegative...
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ExcretionofLivingBorreliarecurrentisinFecesofInfectedHumanBodyLice
...asfixedinabsoluteethanolat4°Cfor3weeks,toincreasethefixationefficiency,andembeddedinparaffin.IFAwasperformedon5-m-thicksectionsbyuseof30LofmouseantiB.recurrentisstrainA1polyclonalantibody(PAb)dilutedtoaconcentrationof1:250inPBSwith3%(wt/vol)nonfatdrymilk.Sectionsofuninfectedlicewereusedasnegativeco...
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Granulocyte-MacrophageColonyeCStimulatingFactorandLungImmunityinPulmonaryAlveolarProteinosis
...conductedafterobtainingwritten,informedconsentfromeachparticipantbetweenDecember2000andOctober2002.WetreatedaseriesofthreeindividualswithiPAP(onemanandtwowomen;agerange,51eC57years)withaerosolizedGM-CSF(Table1).BAL,transbronchiallungbiopsy,andantieCGM-CSFantibodyintheserumconfirmedtheiPAPdiagnosis.T...
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TreatmentWithStatinsAfterInductionofFocalIschemiainRatsReducestheExtentofBrainDamage
...edinCarnoyreagent(absoluteethanol:chloroform:glacialaceticacid,6:3:1;Merck)embeddedinParaplast(SIGMA),and5-µmcoronalsectionswerestainedwithHEandexaminedbylightmicroscopy.FortheimmunohistochemicalstudiesofeNOS,paraffin-embeddedslidesweredewaxedinxyleneandrehydratedusingethanol100%,96%,and75%and...
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SubacuteSclerosingPanencephalitis:MoreCasesofThisFatalDiseaseArePreventedbyMeaslesImmunizationthanWasPreviouslyRecognized
...ens.SpecimensthatunderwentRT-PCRandsequencingincludedfresh-frozen,paraffin-embedded,orformalin-fixedbraintissuespecimensthathadbeenobtainedafterdeathorduringbiopsy.RNAwasextractedfromparaffin-embeddedtissuesamplesaccordingtotheproceduredescribedbyKoopmansetal.[26].RNAwasextractedfromfresh-frozenorfo...
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Interleukin-ßandSignalingofInterleukin-inVascularWallandCirculatingCellsModulatestheExtentofNeointimaFormationinMice
...malinbeforetheligatedorsham-operatedcarotidarterywasharvested,processed,andembeddedintoparaffinwax.Five-µm-thicksectionsweretakenalongtheentirelengthofeacharteryatintervalsof100µm.Typically,thisyielded10sectionsperartery.ThesesectionswerehistologicallystainedwithAlcianblue/elasticvanGies...
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Roleoffibrillin-1inhypertensiveanddiabeticglomerulardisease
...iatelysnapfrozeninliquidnitrogenforlaterRNAextraction,whereasasecondpartwasembeddedinTissueTek(Sakura,Zoeterwoude,TheNetherlands)andfrozeninliquidnitrogenforcryostatsections.Anotherpartwastransferredtomethyl-Carnoysolution(60%methanol,30%chloroform,and10%glacialaceticacid)forfixation.Tissuesweredehy...
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Peritubularcapillarydysfunctionandrenaltubularepithelialcellstressfollowinglipopolysaccharideadministrationinmice
...orreal-timePCR,andtheotherwasfixedin10%phosphate-bufferedformalin.Paraffin-embeddedsections(3µm)werestainedbytheperiodicacid-Schiffreactionforanalysisofmorphologyandneutrophilinfiltrationbyablindedobserver.SerumBloodUreaNitrogenandCreatinineDeterminationBloodureanitrogen(BUN)andcreatinineconce...
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Counter-RegulatoryFunctionofProteinTyrosinePhosphatase1BinPlatelet-DerivedGrowthFactor-orFibroblastGrowthFactor-InducedMotilityandProliferati
...swerefixedbyinsituperfusionthroughtheleftventriclewith10%formalinfollowedbyembeddinginparaffin.Cross-sectionswerestainedwithhematoxylinandeosinformorphometricanalysis.ImageswerecollectedbyusingSpot3.3.2software.Thecrosssectionalsurfaceareasofneointimaandmediaweremeasuredbyusingacomputerizedimageanal...
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DetectionandLocalizationofPrPScintheSkeletalMuscleofPatientswithVariantIatrogenicandSporadicFormsofCreutzfeldt-JakobDisease
...dthesamehigh-sensitivityWesternblottingtechnique,incombinationwithparaffin-embeddedtissueblotting,toscreenforPrPScinmuscletissuespecimenstakenatautopsyfrom49CJDpatientsintheUnitedKingdom.ThesetechniquesidentifiedmusclePrPScin8of17vCJD,7of26sCJD,and2of5iCJDpatients.Paraffin-embeddedtissueblottinganal...
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NovelRoleofARF6inVascularEndothelialGrowthFactoreCInducedSignalingandAngiogenesis
...onsesaremediatedareincompletelyunderstood.ADP-ribosylationfactor6(ARF6)isamemberoftheRassuperfamilyofsmallGTPasesandisinvolvedinmembranetrafficking,Rac1-mediatedmembraneruffling,corticalactinremodeling,cellmotility,andactivationofphospholipaseD.8ARF6alsocolocalizeswithRac1inendosomes,andthetwoaresim...
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DeepVeinThrombosisResolutionIsModulatedbyMonocyteCXCR2-MediatedActivityinaMouseModel
...ationTrichromeStainingImmunohistochemicalstainingwasperformedontheparaffin-embeddedtissuesections(10μm)asdescribed.8–10,14Anti-PMN(1:1000;AccurateChemicalandScientificCorp,Westbury,NY),anti-F4/80(1:100;Serotec,Oxford,UK),anti-laminin(1:100;SantaCruzBiotechnology,SantaCruz,Calif),anti-vonWillebran...
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BoneMorphogeneticProtein-InhibitorGremlinIsOverexpressedinIdiopathicPulmonaryFibrosis
...issuedestructionandactiveareasoffibroproliferationinthelung.Gremlin(Drm),amemberofthecysteineknotfamilyofbonemorphogeneticprotein(BMP)inhibitors,functionstoantagonizeBMP-4-mediatedsignalsduringlungdevelopment.WedescribehereconsistentoverexpressionofgremlininthelunginterstitiumofIPFpatients.Quantitat...
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ChronicOxidativeStressCausesAmplificationandOverexpressionofptprzProteinTyrosinePhosphatasetoActivate??-CateninPathway
...ffer(Ambion,Austin,TX)aspreviouslydescribed.20Eitherformalin-fixedparaffin-embeddedsectionsorcelllineswereusedonMAS-GP-coatedglassslides(MatunamiGlassInd.,Ltd.,Kishiwada,Japan)aftersmearpreparation.GeneExpressionMicroarrayAtotalof10microarrays(RatGenome2302.0;31,999genes;AffymetrixInc.,SantaClara,CA...
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AT1receptorblockadepreventsinterstitialandglomerularapoptosisbutnotfibrosisinpigswithneonatalinducedpartialunilateralureteralobstruction
...-cmlongitudinalslitwasmadeintheadjacentpsoasmuscle.Theobstructionwascreatedembeddingthemobilizedureterinthemuscleslitandclosingthemusclewiththree5-0nylonsutures.Inthesham-operatedgroups,theureterwasexposedbutnotobstructed.Inone-halfofthepartialobstructedandinone-halfofthesham-operatedpiglets,theAT1r...
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RosuvastatinTreatmentPreventsProgressiveKidneyInflammationandFibrosisinStroke-ProneRats
...frenalcortexwerefixedovernightinDuboscq-Brazilfluid,dehydratedinalcohol,andembeddedinparaffin,and3-µmsectionswerecutandstainedwithhematoxylinandeosin,Masson??strichrome,orperiodicacid-Schiffreagent.Vascularchangeswerescored0(absent),1(mildhyalinedepositionandwideningofintercellularspaceintheme...
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HepatocyteGrowthFactorAttenuatesLiverFibrosisInducedbyBileDuctLigation
...eviouslydescribed.26,27HistologyandImmunostainingLiversectionsfromparaffin-embeddedtissueswerepreparedat3-µmthicknessusingaroutineprocedure.Sectionswerestainedwithhematoxylin/eosinforgeneralhistology.AnothersetofsectionswasstainedusingtheMasson??strichromestainingmethodforidentifyinginterstiti...
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LuteogenicHormonesActthroughaVascularEndothelialGrowthFactor-DependentMechanismtoUp-RegulateßandvßIntegrinsPromotingtheMigration
...lpreparationstoensureconsistencyofthefindings.ImmunohistochemistryParaffin-embeddedhumanovariantissuesectionsweredeparaffinizedat70??Cinxylene,rehydrated,andrinsedinphosphate-bufferedsaline(PBS)(pH7.4).Sectionsweretreatedwith0.1%hydrogenperoxidefor30minutesat20??C,washedinPBS,anddigestedwithprewarme...
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Lowendogenousglucocorticoidallowsinductionofkidneycorticalcyclooxygenase-2duringpostnatalratdevelopment
...1.5h).DoubleimmunofluorescencelabelingforGR-andCOX-2wasperformedonparaffin-embeddedratkidneysectionsfromP10.Sectionswereincubatedwithanti-COX-2(1:500),anti-GR(1:100),anti-THP(1:100),andanti-11HSD-2(1:1,000)antibodiesfor16hat4°Cin5%drymilkinTTBS.Afterbeingwashedseveraltimes,thesectionswereincubat...
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RoleofInterleukin0duringPersistentInfectionwiththeRelapsingFeverSpirocheteBorreliaturicatae
...otherhalfwasfixedin4%paraformaldehydefor6hoursatroomtemperatureandparaffin-embedded.IL-10TreatmentToassesstheeffectofIL-10ondisease,groupsoffour4-to5-week-oldfemaleIgh6C/CmicepersistentlyinfectedwitheitherBt1orBt2weretreatedintraperitoneallywitheither1)dailydosesof50µg/kgmurinerIL-10(Pierce,Ro...
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CharacterizationofaNewSpeciesofAdenovirusinFalcons
...atehomeinSanDiego,Calif.,thatalsohousedperegrinefalcons.ThisanimaldiedinDecember2001.Twoanimalswerejuvenileteitafalcons(Falcofasciinucha),andtwojuvenileanimalsweregyrfalconxperegrinefalconhybrids(Falcorusticolus/peregrinus)thatwerecohousedinafacilityinOklahomaanddiedinFebruary2002.Oneanimalwasajuven...
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ReversalofexperimentalpulmonaryhypertensionbyPDGFinhibition
...nsentwasobtainedfromeachindividualpatientorthepatient’snextofkin.Paraffinembeddingandmicroscopy.Fixationwasperformedbyimmersionofthelungsina3%paraformaldehydesolution.Forparaffinembedding,theentirelungsweredissectedintissueblocksfromalllobes.Sectioningat3μmwasperformedfromallparaffin-embeddedbloc...
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CCChemokineReceptorExpressionbyBLymphocytesIsEssentialfortheDevelopmentofIsolatedLymphoidFollicles
...Isolatedlymphoidfollicles(ILFs)haveonlyrecentlybecomeappreciatedasdistinctmembersofthegastrointestinal-associatedlymphoidtissues.ILFsresemblePeyer??spatches(PPs)intheirarchitecture,cellularcomposition,andabilitytoactasinductivesitesformucosalimmuneresponses.1-5Ourunderstandingofhowtheseorganizedstru...
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EnhancedPIP3signalinginPOMCneuronscausesKATPchannelactivationandleadstodiet-sensitiveobesity
...hanoltoenhanceultrastructuralmembranecontrast.Dehydrationwasfollowedbyflat-embeddinginAraldite.UltrathinsectionswerecutonaLeicaultramicrotome,collectedonFormvar-coatedsingle-slotgrids,andanalyzedwithaTecnai12Biotwin(FEICompany)electronmicroscope.OnlyPOMC-immunolabeledcellswithequalimmunoperoxidasede...