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DuplexReverseTranscription-PCRFollowedbyNestedPCRAssaysforDetectionandIdentificationofBrazilianAlphavirusesandFlaviviruses
...irus(PIXV,subtypeIV),Mayarovirus(MAYV),Unavirus,andflavivirusesdenguevirus(DENV,subtypes1to4),SaintLouisencephalitisvirus(SLEV),Bussuquaravirus(BSQV),Cacipacorevirus,Iguapevirus,Ilheusvirus(ILHV),Rociovirus(ROCV),andyellowfevervirus(YFV)havebeenisolatedfrommosquitoes,animals,orhumans(1,15,37).Thesea...
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FlowCytometry-BasedAssayforTitratingDengueVirus
...rolina,ChapelHill,NorthCarolinaABSTRACTPlaqueassaysfortitratingdenguevirus(DENV)aretime-consumingandnotsuitableforstrainsthatdonotplaque.Fluorescence-activatedcellsorting(FACS)hasbeenusedtodetectDENV-infectedcells.HerewedescribeaFACS-basedassayfortitratingDENV.Wedeterminedthatat24hpostinfection,then...
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Nature：登革热病毒的感染能力
...其他地方是一个新出现的威胁。它是由四种登革热病毒（DENV-1、2、3和4）中的其中一种引起的，由斑蚊传播；目前没有得到批准的疫苗，也没有有效的特效疗法。登革热病毒是小型黄病毒，很可能需要大量宿主因子才能传播，因...
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ImprovedMolecularDetectionofDengueVirusSerotype1Variants
...51,1992)isusedworldwidefordiagnosisandsurveillance.However,itfailedtodetectDENV-1variantsinCambodiaduetoapointmutation.WedescribeanimprovementofthemethodthatallowsthedetectionofadditionalDENV-1strains,includingpotentialvariants. TEXTTopAbstractTextReferencesDenguevirus(Flaviviridae,genusFlavivirus...
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ComparativeAnalysisofImmunoglobulinM(IgM)CaptureEnzyme-LinkedImmunosorbentAssayUsingVirus-LikeParticlesorVirus-InfectedMouseBrainAntigensTo
...s,wehavedevelopedaeukaryoticplasmidvectortoexpressthepremembrane/membraneandenvelopeproteinswhichself-assembleintononinfectiousvirus-likeparticles(VLPs).InadditiontotheplasmidsforJapaneseencephalitisvirus,WestNilevirus(WNV),St.Louisencephalitisvirus(SLEV),anddenguevirustype2(DENV-2)reportedearlier,w...
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福州市2004年登革热流行病学和病原学特征分析
...病毒基因的核苷酸序列比对证明同源性与柬埔寨分离株(DENV-1/KHM/2001,GenBankAccessionNo.L0904278)最为接近。可见福州市2004年登革热疫情为感染登革1型病毒所引起；疫情的发生主要为输入性病例流行引起。作者：
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登革热流行严重程度的关键因素被确定
...次的登革热感染——当其第二次感染了该病毒的一种叫做DENV-2的亚组时却会死于该病的出血热形式。文章的作者先前对2004-2005年及2008-2009年在尼加拉瓜的马那瓜的登革热流行开展了两项独立的临床研究。该团队用来自这些试验的...
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Occasionalphysicalinactivitycombinedwithahigh-fatdietmaybeimportantinthedevelopmentandmaintenanceofobesityinhumansubjects
...cineandBiometrics,andofPediatrics,UniversityofColoradoHealthSciencesCenter,Denver.2SupportedbygrantDK-46881fromtheNationalInstituteofDiabetesandDigestiveandKidneyDiseases,grantP30DK-48520-01fromtheColoradoClinicalNutritionResearchUnit(MetabolicandEnergyBalanceLaboratories),andgrantMO1-RR00051fromthe...
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HerpesSimplexVirusType2InfectionasaRiskFactorforHumanImmunodeficiencyVirusAcquisitioninMenWhoHaveSexwithMen
...icine,2Epidemiology,and3LaboratoryMedicine,UniversityofWashington,Seattle;4DenverDepartmentofPublicHealth,Denver,Colorado;5AIDSOffice,SanFranciscoDepartmentofPublicHealth,SanFrancisco,California;6LaboratoryofEpidemiology,NewYorkBloodCenter,NewYork;7DepartmentofPsychology,UniversityofIllinoisandHowar...
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ACatalyticAntioxidantAttenuatesAlveolarStructuralRemodelinginBronchopulmonaryDysplasia
...searchCenter;DepartmentofMedicine,UniversityofColoradoHealthSciencesCenter,Denver,Colorado;DepartmentofPediatrics,UniversityofTexasHealthSciencesCenter;SouthwestFoundationforBiomedicalResearch,SanAntonio,Texas;DepartmentofPathology,HarvardMedicalSchool;DepartmentsofPathology,Children‘sHospital;and...
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职业睡眠学会第19届年会热点(2005－6)
...nnualMeeting2005年6月18－23日美国科罗拉多州丹佛June18-23,2005,Denver,ColoradoAdvancesinUnderstandingtheNatureofInsomniaThomasRoth,PhDTheAssociationofProfessionalSleepSocieties(APSS)heldits19thannualmeetingonJune16-23,2005,inDenver,Colorado.Insomniawasamajorfocusatthisyear‘sconference;...