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小窝蛋白1、基质金属蛋白酶2在膀胱癌的表达及其关系
ExpressionofCAV1andMMP2inbladdercancerandtherelationshipbetweenthem LiHengping,ChenYirong,YanDongwen,ShiBaoguang,GuoYingfang (DepartmentofUrology,GansuCancerHospital;DepartmentofUrology,thePeoplesHospitalofGansuProvince;DepartmentofPathology,thePeoplesHospitalofGansuProvince,La...
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脊髓Cav3．2型钙通道在大鼠慢性神经病理性痛形成中的作用
...医学空间（MEDcyber.com）3月12日消息，该项研究目的是探讨Cav3.2型钙通道在大鼠慢性神经病理性痛形成中的作用。方法采用健康雄性SD大鼠,制备大鼠背根神经节慢性压迫（CCD）模型,选取鞘内置管成功的32只大鼠,随机分为4组（n=8）...
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测定钙通道的相互作用热点区域
电压门控性钙通道(CaVs)是由多个亚基组成的大的复合体，控制钙进入细胞。而电压门控性钙通道的致孔剂(CaVα1)和细胞质亚基(CaVβ)间的相互作用，是通过CaVα1的α交互作用区(AID)和CaVβ的α结合口袋(ABP)的高亲和力进行的。在最新...
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清华大学颜宁教授Science再发重要新成果
...华大学的研究人员报道称，她们分离出了来自兔骨骼肌的Cav1.1复合物，并采用单颗粒冷冻电镜技术借助直接电子检测和先进的图像处理确定了它的结构。获得这一伪四聚体真核生物Cav通道与其辅助亚基的复合物的详细结构，为认...
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Directevidencefortheroleofcaveolin-1andcaveolaeinmechanotransductionandremodelingofbloodvessels
...artmentofPathology,DartmouthMedicalSchool,Hanover,NewHampshire,USA.AbstractCaveolaeinendothelialcellshavebeenimplicatedasplasmamembranemicrodomainsthatsenseortransducehemodynamicchangesintobiochemicalsignalsthatregulatevascularfunction.Thereforewecomparedlong-andshort-termflow-mediatedmechanotransdu...
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GeneticAblationofCaveolin-1ConfersProtectionAgainstAtherosclerosis
...monocytes/macrophagesandsmoothmusclecells.Here,weevaluatethepotentialroleofcaveolaemembranedomainsinthepathogenesisofatherosclerosisbyusingapolipoproteinE-deficient(ApoE-/-)miceasamodelsystem.MethodsandResults—Caveolin-1(Cav-1)isaprincipalstructuralproteincomponentofcaveolaemembranedomains.Todirec...
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Caveolin-(C/C)-andCaveolin-(C/C)-DeficientMiceBothDisplayNumerousSkeletalMuscleAbnormalitieswithTubularAggregateFormation
【摘要】Here,weexaminetheroleof“non-muscle“caveolins(Cav-1andCav-2)inskeletalmusclebiology.OurresultsindicatethatskeletalmusclefibersfrommaleCav-1(C/C)andCav-2(C/C)miceshowstrikingabnormalities,suchastubularaggregates,mitochondrialproliferation/aggregation,andincreasednumbersofM-cadherin-pos...
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儿童咳嗽变异性哮喘发病的饮食危险因素分析及护理
【摘要】目的分析儿童咳嗽变异性哮喘(CAV)发病的饮食危险因素，以便给予相应的护理措施。方法采取病例对照研究策略，选取已确诊为CAV患儿54例作为病例组，于同群体、同时间选取无免疫系统疾病及慢性咳嗽的其他系统疾病...
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StromalandEpithelialCaveolin-BothConferaProtectiveEffectAgainstMammaryHyperplasiaandTumorigenesis
【摘要】Here,weinvestigatetheroleofcaveolin-1(Cav-1)inbreastcanceronsetandprogression,withafocusonepithelial-stromalinteractions,ie,thetumormicroenvironment.Cav-1ishighlyexpressedinadipocytesandisabundantinmammaryfatpads(stroma),butitremainsunknownwhetherlossofCav-1withinmammarystromalcellsaffec...
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内皮小窝蛋白-1参与结肠炎小鼠血管生成调节
...路易斯安娜大学《胃肠病学》报道，血管内皮小窝蛋白-1(Cav-1)可能是结肠炎小鼠血管增生调节的关键因素。人类炎症性肠病（IBDs）和结肠炎动物模型都有血管增生现象。小窝(caveolae)为一种非包被的细胞小囊/小泡,有胞膜小窝、...
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Caveolin-Deficiency(C/C)ConveysPremalignantAlterationsinMammaryEpitheliawithAbnormalLumenFormationGrowthFactorIndependenceandCellInvasivenes
...turesofmammaryepithelialcellsderivedfromgeneticallyengineeredmicetoidentifycaveolin-1(Cav-1)asacriticalfactorformaintainingthenormalarchitectureofthemammaryacinarunit.Isolatedculturesofnormalmammaryepithelialcellsretainedthecapacitytogeneratemammaryaciniwithinextracellularmatrix.However,thosefromCav...
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CAV1基因突变-肾移植失败的重要促发因素
...生纤维性结缔组织的一种抑制物。根据文章的背景资料，CAV1基因与组织纤维化以及血管增长有关，这些都是肾移植失败的重要促发因素。文章的作者写道：“迄今没有研究阐明CAV1基因变异是否会增加一般性纤维化或是特异性肾...
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鸡贫血病毒VP2基因的克隆、序列分析及表达
...连接将其亚克隆到PET载体上并进行原核表达。结果：发现CAV-SJ1株的VP2基因共有651个碱基，同国外TK5803、26P4、Cux-1、82-2毒株的VP2基因相比分别有6、7、8、7个碱基的差别，其中3个是SJ1株特有的碱基变化。由基因序列推导出的CAV-SJ1...
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Caveolin-and-ßPerformNonredundantRolesinEarlyVertebrateDevelopment
【摘要】Caveolinsareintegralmembraneproteinsthatlocalizepredominantlytolipidrafts,wheretheyoligomerizetoformflask-shapedorganellestermedcaveolaeandplayimportantrolesinmembranetrafficking,signaltransduction,andothercellularprocesses.Toinvestigatepotentialrolesforcaveolin-1(cav-1)indevelopment,cav...
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CAV1基因突变-肾移植失败的重要促发因素
...生纤维性结缔组织的一种抑制物。根据文章的背景资料，CAV1基因与组织纤维化以及血管增长有关，这些都是肾移植失败的重要促发因素。文章的作者写道：“迄今没有研究阐明CAV1基因变异是否会增加一般性纤维化或是特异性肾...
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Cav1.1基因26内含子67位点A/G多态性与甲状腺功能亢进性周期性瘫痪的相关性研究
...杂志2006Vol.39No.6P.396-3988（青岛）为了探讨钙通道α1亚基(Cav1.1)基因26内含子-67A/G多态性与男性甲状腺功能亢进(简称甲亢)性周期性瘫痪(TPP)的相关性。研究者采用多聚酶链反应-单链构象多态性(PCR-SSCP)方法检测46例男性TPP患者(TPP组)...
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应用31P磁共振化学移位成像法对心脏移植物血管病变进行无创性诊断
...5-9.78冠状动脉造影术目前仍是诊断心脏移植物血管病变（CAV）的金标准，而当前对于这种病变的无创性诊断方法也正在研究当中。法国马赛大学医学院的CausT博士及其同事在成年心脏移植受者群体中通过研究心脏高能磷酸盐的变...
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Low-Voltage-Activated(T-Type)CalciumChannelsControlProliferationofHumanPulmonaryArteryMyocytes
...ularstudieshaveidentifiedmultipleCa2+channelgenesexpressedinmammaliancells.Cav3.xgenefamilymembers,encodinglowvoltage-activated(LVA)orT-typechannels,werefirstidentifiedinthecentralnervoussystemandsubsequentlyinnon-neuronaltissue.ReportsofapotentialroleforT-typeCa2+channelsincontrollingcellproliferat...
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CaV2.3channelandPKC:newplayersininsulinsecretion
...ecretioniscriticallydependentontheproperfunctionofacomplexmolecularnetwork.CaV2.3-knockout(CaV2.3–/–)andPKC-knockout(PKC–/–)mousemodelsnowsuggestthatthese2players,theCav2.3channelandPKC,areimportantconstituentsofthismolecularnetwork.Subsequenttoglucosestimulation,insulinisreleasedfromthepanc...
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cAblTyrosineKinaseMediatesReactiveOxygenSpecieseCandCaveolin-DependentAT1ReceptorSignalinginVascularSmoothMuscle
...es(ROS)derivedfromNAD(P)Hoxidase(NOX)andisdependentonAT1Rtraffickingthroughcaveolin1(Cav1)-enrichedlipidrafts.Underlyingmolecularmechanismsareincompletelyunderstood.Thenonreceptortyrosinekinase,proto-oncogenecAblisasubstrateofSrcandisamajormediatorforROS-dependenttyrosinephosphorylationofCav1.Wethus...
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Caveolin-MutationsinHumanBreastCancerFunctionalAssociationwithEstrogenReceptor-PositiveStatus
...portedthatupto16%ofbreastcancersamplesharborasporadicmutationwithinthehumanCav-1gene,namelyP132L.Todate,however,nostudieshaveexaminedtheUnitedStates??population.Here,wedevelopedanovelallele-specificreal-timePCRassaytodetecttheCav-1P132Lmutationinmammarytumorcellsisolatedbylasercapturemicrodissection...
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Theβ1SubunitofL-TypeVoltage-GatedCa2+ChannelsIndependentlyBindstoandInhibitstheGatingofLarge-ConductanceCa2+-ActivatedK+Channels
...teractionbetweentheβ1-subunitofL-typevoltage-activatedcalciumchannels(Cavβ1)andcriticalregulatorydomainsofSlo1thatcanoccurintheabsenceofotherproteins.Thisinteractionwasidentifiedbyayeasttwo-hybridscreen,anditwasconfirmedbyconfocalmicroscopyinnativeneurons,bycoimmunoprecipitation,andbydirec...
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EmergenceofaR-TypeCa2+Channel(CaV2.3)ContributestoCerebralArteryConstrictionAfterSubarachnoidHemorrhage
...ughtheemergenceofR-typevoltage-dependentCa2+channels(VDCCs)encodedbythegeneCaV2.3.Usinginvitrodiametermeasurementsandpatchclampelectrophysiology,wehavefoundthatL-typeVDCCantagonistsabolishcerebralarteryconstrictionandblockVDCCcurrentsincerebralarterymyocytesfromhealthyanimals.However,5daysafterthein...
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AntihypertensiveEffectsofthePutativeT-TypeCalciumChannelAntagonistMibefradilAreMediatedbytheL-TypeCalciumChannelCav1.2
...ation,time-andtissue-specificconditionalknockoutmodeloftheL-typeCa2+channelCav1.2(Cav1.2SMAKOmice)togeneticallydissecttheeffectsofmibefradilonT-andL-typeCa2+channels.Myogenictoneandphenylephrine-inducedcontractioninhindlimbperfusionexperimentsweresensitivetomibefradilincontrolmice,whereasthedrugshow...
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Roleofcaveolin-1intheregulationofthevascularshearstressresponse
...undernormalconditions,capableofrespondingtoanyvariationinhemodynamicforces.Caveolae—50-to100-nmplasmamembraneinvaginationspresentatthesurfaceofterminallydifferentiatedcellsandparticularlyenrichedinECs—arecomposedofahighsphingolipidandcholesterolcontentandtheproteincaveolin-1(Cav-1).Previousstudi...
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CaV2.3calciumchannelscontrolsecond-phaseinsulinrelease
...icsofinsulinreleasefrompancreaticislets.HerewehaveelucidatedtheroleofR-typeCaV2.3channelsinthatprocess.A20%reductioninglucose-evokedinsulinsecretionwasobservedinCaV2.3-knockout(CaV2.3–/–)islets,closetothe17%inhibitionbytheR-typeblockerSNX482butmuchlessthanthe77%inhibitionproducedbytheL-typeCa2+c...
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Caveolin-1RegulatesStore-OperatedCa2+InfluxbyBindingofItsScaffoldingDomaintoTransientReceptorPotentialChannel-1inEndothelialCells
【关键词】Caveolin-1associatesCaveolin-1associateswithstore-operatedcationchannels(SOC)inendothelialcells.Weexaminedtheroleofthecaveolin-1scaffoldingdomain(CSD)inregulatingtheSOC[i.e.,transientreceptorpotentialchannel-1(TRPC1)]inhumanpulmonaryarteryendothelialcells(HPAECs).Weusedthecellpermeant...
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T-TypeCalciumChannelsAreInhibitedbyFluoxetineandItsMetaboliteNorfluoxetine
...nnels(Tchannels)expressedintsA201cells.FluoxetineinhibitedthethreeTchannelsCaV3.1,CaV3.2,andCaV3.3inaconcentration-dependentmanner(IC50=14,16,and30µM,respectively).Norfluoxetinewasamorepotentinhibitorthanfluoxetine,especiallyontheCaV3.3Tcurrent(IC50=5µM).ThefluoxetineblockofTchannelswasv...
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FunctionalcaveolaeareaprerequisiteforCD40signalinginhumanrenalproximaltubulecells
【摘要】TheroleofcaveolaeinCD40/CD154activationofproinflammatorychemokinesandtheirpotentialroleinrenalinflammatorydiseasewereexploredinprimaryculturesofhumanrenalproximaltubuleepithelialcells.Withtheuseofacellfractionationassay,caveolin-1(Cav-1),thedefiningstructuralproteinofcaveolae,wasdetected...
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Neurokinin1ReceptorsTriggerOverlappingStimulationandInhibitionofCaV2.3(R-Type)CalciumChannels
【关键词】Neurokinin1Neurokinin(NK)1receptorsandCaV2.3calciumchannelsarebothexpressedinnociceptiveneurons,andmicelackingeitherproteindisplayalteredresponsestonoxiousstimuli.Here,weexaminedmodulationofCaV2.3throughNK1receptorsexpressedinhumanembryonickidney293cells.WefindthatNK1receptorsgenerate...