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PositiveselectionoftheperipheralBcellrepertoireingut-associatedlymphoidtissues
...MicrobiologyandImmunology,StritchSchoolofMedicine,LoyolaUniversityChicago,Maywood,IL60153Gut-associatedlymphoidtissues(GALTs)interactwithintestinalmicrofloratodriveGALTdevelopmentanddiversifytheprimaryantibodyrepertoire;however,themolecularmechanismsthatlinktheseeventsremainelusive.Aliciarabbitsprov...
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MolecularEpidemiologyofSevereAcuteRespiratorySyndromeAssociatedCoronavirusInfectionsinTaiwan
...Hospital,NationalDefenseMedicalCenterDepartmentofMedicalResearch,TaipeiMackayMemorialHospitalNationalHealthResearchInstitutesInstituteofBiomedicalSciences,AcademiaSinica,Taiwan,RepublicofChinaBackground.In2003,Taiwanexperiencedaseriesofoutbreaksofsevereacuterespiratorysyndrome(SARS)and1laboratory-co...
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AMouseModelforStudyingtheInteractionofBisdioxopiperazineswithTopoisomeraseIIinVivo
...dhomologousrecombination.ThesecellswereusedforthegenerationoftransgenicTOP2AY165S/+mice,whichweredemonstratedtoberesistanttowardthegeneraltoxicityofbothICRF-187andICRF-193.HematologicalmeasurementsindicatethatthisismostlikelycausedbyadecreasedabilityoftheseagentstoinducemyelosuppressioninTOP2AY165S/...
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ComparisonbetweenrpoBand16SrRNAGeneSequencingforMolecularIdentificationof168ClinicalIsolatesofCorynebacterium
...cationofanyclinicalisolateofaCorynebacteriumsp.isimportant(3).However,thismaynotbeeasyifconventionalmethodsareusedastheymayrequireusingsuchfastidiousproceduresaschromatography.Furthermore,commerciallyavailablebiochemicaltestsystems(e.g.,APIstrips[Biomerieux,Marcyl‘Etoile,France])maynotincorporatea...
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Nocardiaignorata,aNewAgentofHumanNocardiosisIsolatedfromRespiratorySpecimensinEuropeandSoilSamplesfromKuwait
...ocardiaspp.areubiquitousintheenvironment,theirisolationfromclinicalsamplesmaybespuriousorsimplyrevealrespiratorycolonization(18).Incontrast,isolatesOFN03.01.4andOFN00.18werebothclearlyresponsibleformultiplenecrotizingpulmonaryabscesses.Directexaminationofsputum(patient1)andbronchoalveolarlavagefluid...
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ComparisonofTwoTargetGenesforDetectionandGenotypingofGiardialambliainHumanFecesbyPCRandPCR-RestrictionFragmentLengthPolymorphism
...edePharmacie,5rueAlbertLebrun,BP80403,54001NancyCedex,FranceABSTRACTAPCRassaytargetingthetpigenewasdevelopedtodetectandtogenotypeGiardialambliainhumanfeces.OurassaywasspecificanddiscriminatedbetweenG.lambliaassemblagesAandB.G.lambliacystsisolatedfromhumanfeceswerealsoanalyzedwithtwopreviouslydescrib...
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DevelopmentofaDNAMicroarrayforDetectionandIdentificationofFungalPathogensInvolvedinInvasiveMycoses
...earlyinitiationofanadequateantifungaltherapy.Wedevelopedadiagnosticmicroarrayfortherapidandsimultaneousidentificationofthe12mostcommonpathogenicCandidaandAspergillusspecies.Oligonucleotideprobesweredesignedbyexploitingthesequencevariationsoftheinternaltranscribedspacer(ITS)regionsoftherRNAgenecasset...
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RapidandAccurateIdentificationofHumanIsolatesofPasteurellaandRelatedSpeciesbySequencingthesodAGene
...tedfromahumanclinicalspecimen.ThesodAintsequencesoftheclinicalisolatesdisplayedlessthan2.5%divergencefromthoseofthecorrespondingtypestrains,exceptforthePasteurellapneumotropicaisolates,whichwerecloselyrelatedtoeachother(98%sodAintsequenceidentity)butsharedonly92%sodAintidentitywiththetypestrain.Them...
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AmongBcellnon-Hodgkin‘slymphomas,MALTlymphomasexpressauniqueantibodyrepertoirewithfrequentrheumatoidfactorreactivity
...ThelowtendencyofMALTlymphomastospreadbeyondtheenvironmentinwhichtheyevolvemayberelatedtotheexpressionofcertainhomingandchemokinereceptors,suchas47andCXCR3(12,13).Inaddition,ithasbeenproposedthatevenduringthetumorstage,antigen(Ag)playsagrowth-sustainingrole.Thisnotionisstronglysupportedbytheobservati...
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MolecularEpidemiologyofHepatitisCVirusGenotype4IsolatesinEgyptandAnalysisoftheVariabilityofEnvelopeProteinsE1andE2inPatientswithChron
...e,mainlyasaresultofimmunepressure(47,51,54).Thesecoexistingmutantgenomesalwayshaveaconsensus,ormaster,sequence.Ingeneral,despitethepotentiallyhighmutationrateandvariabilityofRNAviruses,changesintheconsensussequenceofaviralpopulationoccuronlyifthepopulationequilibriumisalteredbyaselectionmechanism(16...
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SARS冠状病毒E蛋白基因克隆和分析
...大首先完成该病毒全基因组测序并公开于GenBank数据库（AY274119）。本文利用DNA重组技术，对E蛋白基因进行克隆和序列分析，为进一步开展该基因的结构和功能研究奠定了基础。1材料与方法1.1SARS病毒RNA基因组SARS病毒株序号为21170...
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Leanbodymassdepletionisassociatedwithanincreasedlengthofhospitalstay
...mass,asassessedbybioelectricimpedanceanalysis(BIA),andthelengthofhospitalstay(LOS).Fat-freemasscorrelateswithskeletalmusclemass(6),andimprovementofmusclemassbynutritionalinterventiontogetherwithphysicaltrainingmayimprovetheclinicalcourseofdisease(7).Whereasnitrogenbalancehasbeenusedtodeterminetheeff...
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Roleofcholesterolinembryonicdevelopment
...ouslythat3distalinhibitorsofcholesterolsynthesisarehighlyteratogenicinrats.AY9944andBM15766inhibit7-dehydrocholesterolreductase,whichcatalyzesthelaststepofcholesterolsynthesis,andtriparanolinhibits24-dehydrocholesterolreductase,whichcatalyzesthelaststepinanotherpathway.Thesemoleculescauseholoprosenc...
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GeneticDiversityofMultidrug-ResistantMycobacteriumtuberculosisIsolatesandIdentificationof11NovelrpoBAllelesinTaiwan
...gathoroughrapidsingle-nucleotide-polymorphismdetectionmethod,suchasmicroarray,forhigh-throughputtesting.Forisolateswithoutmutations,N-terminalcodon146forlow-levelresistanceandcodon562mightbealsoinvolvedinRMPdrugresistance(30).Anadditionalgene,thearrgene(1,23),foundtobeassociatedwithRMPresistanceinPs...
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IdentificationandFirstReportofInonotus(Phellinus)tropicalisasanEtiologicAgentinaPatientwithChronicGranulomatousDisease
...nterAudieL.MurphyDivision,SouthTexasVeterans‘HealthCareSystem,SanAntonioBaylorCollegeofMedicineTexasChildren‘sHospital,Houston,TexasUniversityofNebraskaMedicalCenter,Omaha,NebraskaForestProductsLaboratory,USDAForestService,Madison,WisconsinSeoulNationalUniversity,Seoul,RepublicofKoreaABSTRACTAlt...
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DuplexReverseTranscription-PCRFollowedbyNestedPCRAssaysforDetectionandIdentificationofBrazilianAlphavirusesandFlaviviruses
...on-PCR(D-RT-PCR),followedbymultiplexnestedPCR(M-N-PCR)ornestedPCR(N-PCR)assaysforspecies-specificidentification.Bythisprotocol,25arboviruseswerespecificallydetectedandidentified.Detectionlevelsbetween101.3and103.550%tissuecultureinfectivedoses(TCID50)/mlofFlavivirusandAlphavirusstrainswereachievedby...
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IdentificationofNovelHumanHepatitisEVirus(HEV)IsolatesandDeterminationoftheSeroprevalenceofHEVinKorea
...fhumanHEVinKorea.Overall,thisstudydemonstratesthatsubclinicalHEVinfectionsmayprevailinhumanpopulationsinKoreaandthatthereisastrongpossibilitythatHEVisazoonoticagent.INTRODUCTIONSincehepatitisEvirus(HEV)wasfirstidentifiedinavolunteerstudyin1983(5),thevirushasbeenconsideredthecausativeagentofenterical...
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CharacterizationofRotavirusStrainsinaDanishPopulation:HighFrequencyofMixedInfectionsandDiversitywithintheVP4GeneofP[8]Strains
...usinfectionseachyear,mostindevelopingcountries(8,24).Rotavirusesaretriple-layeredicosahedralparticles,andtheirgenomesconsistof11segmentsofdouble-strandedRNA.Rotavirusesareclassifiedaccordingtothegeneticandantigenicdiversityofthetwooutercapsidproteins,VP4andVP7.Theseproteinsindependentlyinducetype-sp...
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中国南方地区3株H5N1亚型人禽流感病毒血凝素基因特性
...rnChinawiththeirspecificprofiles.ThemigratorybirdsorothermigratoryfactorsplaymoreimportantroleinHAgenereassortant. Keywords：HumanavianinfluenzavirusH5N1；Genesequence;SoutnernChina 高致病性禽流感病毒H5N1亚型的爆发首先于1996、1997年［1,2］在中国华南地区发生...
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MolecularEvidenceforMultipleHost-SpecificStrainsintheGenusRhinosporidium
...ehosts‘identities.ThisanalysisstronglysuggeststhatthegenusRhinosporidiummaypossessmultiplehost-specificstrains.NocorrelationwasfoundbetweenthisfindingandthephenotypicfeaturesofR.seeberiinthestudiedsamples.INTRODUCTIONRhinosporidiosisisacutaneousand/orsubcutaneouschronicdiseaseofhumanandotheranimal...
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二、其他突变品系
...无毛大鼠，无毛豚鼠，无毛兔（但有胸腺），BN（BrownNorway）棕色小鼠，LongEvans头部毛斑如包头巾、基因型为hh的大鼠和基因型hhaa、尾基部有黑色毛的大鼠，Sherman大鼠等突变品系动物，都已广泛应用于肿瘤、免疫、造血等的研究...
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基因碱基序列分布的余弦拟合法
...四进制”与信息守恒律。1材料与方法1.1基因序列的来源AY644963下载自EMBL的EMBL-Bank，该基因有378个碱基:ATTCAGCAGCCTTTTTCTCATGCAGACTTGTAAATCTGGGAGTCTGTCAGTCAGTCAGTCAGCCAGTCAACTTTTCTGTTGGAAAGTCCGTCTTCATAAAGCCAGCATGTCTCTTGGGGAACCTGGCATTTTGTTAAAATAACAAGTGTTTAATTTTTCAGGGGAA...
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Bypassingcomplement:evolutionarylessonsandfutureimplications
...tcascadeanalogoustothemorefamiliarbutevolutionarilymorerecentclassicalpathway,whichistriggeredbyantibodybindingtoantigen.InthisissueoftheJCI,Selanderetal.developedasensitiveandspecificELISAemployingSalmonella-specificsugarstoassesstheactivityofthelectinpathwayofcomplementactivation.Thismorephysiolog...
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PhenotypicandGeneticCharacterizationofClinicalIsolatesofCDCCoryneformGroupA-3:ProposalofaNewSpeciesofCellulomonas,Cellulomonasdenverensis
...heGeneralMeetingoftheAmericanSocietyforMicrobiology,Washington,D.C.,18to22May2003.)MATERIALSANDMETHODSStrains.TheclinicalisolatesusedinthisstudyweresixstrainsofCDCgroupA-3(Table1).TheywereobtainedfromthecollectionofCDC‘sSBRLandActinomyceteReferenceLaboratory.ThereferencestrainofC.hominis(DMMZCE39)...
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PhylogeneticCharacterizationofCanineDistemperVirusesDetectedinNaturallyInfectedDogsinNorthAmerica
...tein(P).TheHgeneproteinisresponsibleforviralattachmenttothecellhost(38)andmayalsoplayaroleininductionofprotectiveimmunity(11).TheHproteinisoneofthemostvariablemorbillivirusproteinsandthushasoftenbeenusedtoassessgeneticchangesbetweenCDVisolates(8,9,17,18,20,21,23,26,28,30).Inthisstudy,postmortemfindi...
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第三节 荧光免疫测定
...限制。时间分辨荧光免疫测定（timeresolvedfluorescenceimmunoassay，TR-FIA）是针对这缺点加以改进的一种新型检测技术。其基本原理是以镧系元素铕（Eu）螯合物作荧光标记物，利用这类荧光物质有长荧光寿命的特点，延长荧光测量时...
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GenotypingofMadurellamycetomatisbySelectiveAmplificationofRestrictionFragments(AmplifiedFragmentLengthPolymorphism)andSubtypeCorrelationwith
...dan(P=0.001).ThisisthefirstreportonagenotypicstudywheregeneticmarkerswhichmaybeusedtostudypathogenicityinM.mycetomatiswereobtained.INTRODUCTIONInthe1840s,physiciansoftheRoyalArmystationedintheMaduraregioninIndiareportedaninvasivediseasewhichseverelyaffectedthefoot.Thefootdegeneratedinto“onemassofd...
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TheRoleof16SrRNAGeneSequencinginIdentificationofMicroorganismsMisidentifiedbyConventionalMethods
...eCity,UtahARUPLaboratories,SaltLakeCity,UtahLoyolaUniversityMedicalCenter,Maywood,IllinoisABSTRACTTraditionalmethodsformicrobialidentificationrequiretherecognitionofdifferencesinmorphology,growth,enzymaticactivity,andmetabolismtodefinegeneraandspecies.Fullandpartial16SrRNAgenesequencingmethodshaveem...
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QuestionsconcerningtheNewHavenCoronavirus
...heactualnucleotidedifferencebetweentheNewHavenisolates(GenBankaccessionnos.AY870943AY871008)andtheHCoV-NL63isolateAmsterdam-1(GenBankaccessionno.NC_005831)is0%6%.ThisdegreeofdifferencefallswellwithintherangeofgeneticvariationobservedamongdifferentHCoV-NL63isolatesfromAmsterdam[2].Wereportedthepresen...
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OrganotinCompoundsPromoteAdipocyteDifferentiationasAgonistsofthePeroxisomeProliferator-ActivatedReceptor/RetinoidXReceptorPathway
...harmaceuticalSciences,OsakaUniversity,Osaka,JapanAbstractNuclearreceptorsplayimportantrolesinthemaintenanceoftheendocrinesystem,regulationoforgandifferentiation,andfetaldevelopment.Endocrinedisruptorsexerttheiradverseeffectsbydisruptingtheendocrinesystemviavariousmechanisms.Toassesstheeffectsofendoc...