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LongitudinalFollow-upStudyofSmoker’sLungwithThin-SectionCTinCorrelationwithPulmonaryFunctionTests1
...mokers,whoshowedahigherfrequencyofemphysema(40%vs26%;P=.005)andground-glassattenuation(42%vs28%;P=.02).Individualanalysisoffollow-upCTscansinthe19persistentcurrentsmokerswithmicronodulesatT0demonstrated(a)nochangesinsevencases,(b)ahigherprofusionofmicronodulesinsevencases,and(c)replacementofmicronod...
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自体牙移植术的临床效果
...ORDS］Tooth;Transplantation,autologous;Treatmentoutcome自体牙移植术(ATT)是将自体的埋伏牙、阻生牙、错位牙等拔下，在自体牙列中行易位移植。临床上应用较多的是将正在萌出、牙根尚未完全形成的下颌第三磨牙移植到下颌第一磨牙缺失...
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5-3-3位点特异性重组
...；和含有噬菌体和细菌DNA发生重组交叉的特异位点（称为attP和attB，att源自attachment）的DNA片段。对于这个DNA片段，一个简单的制备方法就是人工构建含attP和attB两者质粒。当整合反应发生时，即在attP和attB处发生交叉重组，产生...
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RegularconsumptionofNaFeEDTA-fortifiedfishsauceimprovesironstatusandreducestheprevalenceofanemiainanemicVietnamesewomen
...3).AmorerecentNutritionRiskFactorSurvey,basedondatacollectedin2000,showedthattheprevalenceofanemiahaddecreasedto34%inyoungchildrenandto25%inwomen.However,anemiaremainsasignificantpublichealthprobleminVietnam(HHKhoi,NCKhan,andLBMai,unpublishedobservations,2001),andstrategiesforcombatinganemia,ID,andI...
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5-3-5常见的转位子
...后形成有两个碱基的粘性末端。res位点有些像λ噬菌体的att核心。在交叉点的两侧，这两个位点（res和att）在15bp中有10bp是同源的，如下： res5’GATAATTTAT↓AATAT att--5’GCTTT↑TTTATACTAA 上图中的箭头分别表示TnpR和Int的切割...
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朱棣文能源研究中心计划遭国会拒绝
...越多的低风险想法。”贝尔实验室创建于1925年，当时，ATT总裁华特·基佛德收购了西方电子公司的研究部门，成立了一个叫做“贝尔电话实验室公司”的独立实体。贝尔实验室的工作大致分为三类：基础研究、系统工程和应用开...
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Aprospective,double-blind,placebo-controlledtrialtoestablishasafeglutenthresholdforpatientswithceliacdisease
...Clinical,serologic,andhistologicevaluationsofthesmallintestinewereperformedatt0andaftertheglutenmicrochallenge(t1).Results:Att0,themedianvillousheight/cryptdepth(Vh/Cd)inthesmall-intestinalmucosawassignificantlylowerandtheintraepitheliallymphocyte(IEL)count(x100enterocytes)significantlyhigherintheCD...
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Energyintakeandappetitearerelatedtoantralareainhealthyyoungandoldersubjects
...aprobablypredisposestheelderlytopathologicalanorexiaandmalnutrition(1);thelatterrepresentsamajorcauseofmorbidityandmortalityintheelderly(4,5).Thecauseorcausesoftheanorexiaofagingarepoorlydefined.Gastrointestinalfactorsthatmaydecreaseenergyintakeincludegastricdistension,exposureofsmall-intestinerecep...
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ReplytoKCMcCowen,PRLing,andBRBistrian
...cleandforaddressinganintriguinghypothesis.WeagreethatitwouldbeinterestingtoattempttoconsiderpatientswithCrohndisease,stratifiedforextentofmalabsorption,separatelyfromotherpatientswithfatmalabsorptionintermsoftheconcentrationoftheeicosanoidprecursorsarachidonicandeicosapentaenoicacids.However,thesmal...
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特异性核酸内切酶检测结核分枝杆菌的乙胺丁醇耐药基因突变
...热点306位密码子的点突变，其中33株为ATG→GTG，3株为ATG→ATT，5株为ATG→ATA，2株为ATG→ATC，2株为ATG→CTG。经Surveyor酶法测定，45株embB基因突变株均呈现2条带形，15株敏感株均显示1条带形。可见Surveyor酶法与测序法的结果完全相符...
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WatersQuanta4000型毛细管电泳仪的操作规程
...对WIDTH、SLOPE、MINAREA、DRIFT、LOCK、STOPTIME等峰处理参数和ATT2X、SPEED等记录控制参数进行设置，最后确定样品分析方法。2．设置主机参数：确定样品分析次数，进样时间或进样电压，样品运行时间和电压。3．先用超纯水清洗毛细...
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HLA-B27基因检定技术
...素(HGH)基因特异性引物为内对照：5′-GCC、TTC、CCA、ACC、ATT、CCC、TTA，5′-TCA、CGG、ATT、TCT、GTT、GTG、TTT，扩增产物为429bp。以上引物由美国G&T公司合成、纯化及鉴定。 1.3.3 PCR 将B27引物、内对照引物、MgCl2、Tris、明胶、KCl...
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碧蛾蜡蝉线粒体基因组全序列首次测定
...蝇Drosophilayakuba相同。所有13个蛋白质编码基因均以ATR或者ATT作为起始密码子。9个蛋白质编码基因利用典型的TAA或TAG作为终止密码子，其余四个蛋白质编码基因（cox1,atp6,cox3,和nad4）使用不完全终止密码子T。所有转运RNA(tRNAs)的反...
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促肾上腺皮质激素释放激素受体1在鼠垂体前叶多种内分泌细胞的表达
...分析培养的鼠永生化垂体前叶细胞显示皮质激素类细胞（AtT-20细胞）、促性腺类细胞（T3-1和LβT2细胞）和甲状腺类细胞（TSH细胞）均表达CRH-R1和/或CRH-R2。由于皮质激素类细胞表达CRH-R1的关系已经明确，该研究发现的催乳素类细...
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菝葜含量测定方法（薯蓣皂苷元）
...器温度:300℃;载气流量:0.4L/min;分流比:20∶1;灵敏度:Range=10,att=1;进样量:4μl;纸速:3mm/min。样品溶液的制备：取干燥的菝葜药材适量,粉碎至中粉,加1.5mol/L盐酸水溶液在沸水浴中水解4h,过滤去除酸液,并将药材用水洗至中性,烘干后置索...
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青蒿素项目能否兼顾诺奖和商业？
...明错失了申请专利的良机吗？还是像贝尔实验室的母公司ATT高价雇佣的律师不够高明？当然不是。这样的结果一方面是制度设计造成的，一方面是诺奖级别的科技创新的性质所导致。美国专利法明确规定专利不保护发现自然现象...
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Co-ingestionofaproteinhydrolysateandaminoacidmixturewithcarbohydrateimprovesplasmaglucosedisposalinpatientswithtype2diabetes
...espectively(P0.001).Thereducedplasmaglucoseresponseinthediabeticpatientswasattributedtoa13±3%increaseinglucosedisposal(P0.01).Conclusions:Thecombinedingestionofcarbohydratewithaproteinhydrolysateandaminoacidmixturesignificantlyincreasesdenovoinsulinproductioninpatientswithalong-termdiagnosisoft...
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Bathsheba’sbreastWomen,cancer&history
....95.ISBN:0-8018-8064-5(paperback).JessicaB.MandellUniversityofWashington,Seattle,Washington,USA.E-mail:jmandell@mail.slc.eduIn1654RembrandtpaintedapictureofhismistressentitledBathshebaatherbath.Over300yearslater,anItalianphysicianvacationinginAmsterdamviewedthepaintingattheRijksmuseumandnoticedsever...
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腰硬联合阻滞复合气管内全麻在腹腔镜直肠癌手术中的应用
...hegas(T4).ResultsTheMAP,HR,GluandCoringroupⅠwerehigherthanthoseingroupⅡatT3(P<0.05).ThePETCO2atT3washigherthanthoseatT1andT2(P<0.05).ConclusionThecombinationanesthesiacaneffectivelysuppressthestressreactionduringlaparoscopicradicalresectionofrectalcarcinoma.［KEYWORDS］rectalneoplasms;anesthes...
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Severeimpairmentinliverinsulinsignalingfailstoalterhepaticinsulinactioninconsciousmice
...ofhepaticIRwouldbesufficienttoinducehepaticinsulinresistance.HerewereportthatthedownregulationofhepaticIRexpressionbyupto95%doesnotmodifyhepaticinsulinaction.Thei.p.administration(2injectionsover1week)ofanantisenseoligodeoxynucleotide(ASO)directedtoreduceinsulinexpressiondownregulatedhepaticIRexpres...
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Determinantsofsurgeryrelatedanxietyincataractpatients
...actsurgerywithintraocularlensimplantationintheperiodfromJunetoSeptember2001attheMedicalCentreMaastrichtAnnadal(MCMA,n=50)ortheRotterdamEyeHospital(REH,n=78).Themedicalethicscommitteeapprovedtheresearchproposal,andallpatientssignedaninformedconsentbeforeinclusioninthestudy.Patientswereselectedaccordi...
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DisappointingresultsfromNovaVision’svisualrestorationtherapy
...thatwereblindcannowsee.“4AmajorproblemwiththedatareportedbySabeletalwasthatthesamesoftwareprogramusedforvisualrestorationtherapywasalsousedtoshowimprovementinthevisualfields.Obviously,thedatawouldbemorecompellingifvisualfieldimprovementcouldbedemonstratedwithanystandardclinicalperimeter.Whenpatien...
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亚甲蓝对体内过氧亚硝酸阴离子的清除作用
...oicacid（PABA）weredeterminedduringIPA.ResultsThelevelsofNO,OFR,LPOandMHbatT1andT2（P<0.05or0.01）weresignificantlyincreasedinobservationgroup,thelevelsofSOD,CATandNADH-cyt b5-Rweresignificantlydecreased,thelevelsofNO,OFR,MHb,SOD,CATandNADH-cytb5-RwereallreducedtothenormalatT3（P<0.05or0.01...
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Discriminationanddelusionalideation
...erlands(Thesepopulationsaresubjecttoracialdiscriminationandthereareclaimsthattheassociationbetweenethnicgroupandpsychosisisconfoundedbyracialdiscrimination.AnationalUKstudyhasreportedincreasedratesofcommonmentaldisorderandpsychosisinthosewhosaytheyhavebeenthevictimsofracialdiscrimination.However,ala...
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Validationofbioelectricalimpedanceanalysisinpatientswithamyotrophiclateralsclerosis
...composition,isaninexpensiveandatraumaticmethodthatcanbequicklyandeasilyusedatthepatientbedside(6,12,22).However,thismethodhasbeenincompletelyvalidatedforALSpatients(23).Additionally,thedifferentfrequenciesused(50kHzor5and100kHz)havenotbeencompared.Thus,theaimofthepresentstudywastocomparethereference...
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Criticalroleofstearoyl-CoAdesaturase–1(SCD1)intheonsetofdiet-inducedhepaticinsulinresistance
...ractStearoyl-CoAdesaturase–1(SCD1)catalyzesthesynthesisofmonounsaturatedfattyacidsfromsaturatedfattyacids.MicewithatargeteddisruptionofScd1genelocusareleananddisplayincreasedinsulinsensitivity.ToexaminewhetherScd1activityisrequiredforthedevelopmentofdiet-inducedhepaticinsulinresistance,weusedasequ...
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酒精性肝病合并相关综合征2例报告
...16万/mm3，Hb11g/L，BPC7.4万/mm3。尿常规：蛋白(±)。肝功能：ATT73u/L，AST56u/L，白蛋白26.4g/L，球蛋白28.2g/L，甲胎蛋白(-)，甲肝、乙肝、丙肝、戊肝标志物均阴性。肾功能正常。血糖6.1mmol/L。B超：肝剑突下5.1cm，肋下2.6cm；肝内光点粗...
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机关门诊部心血管病的防治实践
...情况，有的医生甚至给40岁以上的人常规用阿司匹林。在ATT协作的荟萃分析，高危患者长期使用阿司匹林使得复合终点事件如非致命心肌梗死、非致命脑卒中和严重的血管事件降低大约25%，对于高危患者预防血管事件中，阿司匹...
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EpidemiologyandOutcomeofNosocomialandCommunity-OnsetBloodstreamInfection
...BetweenFebruary1999andJuly2000,929consecutiveepisodesofbloodstreaminfectionattwotertiarycarecenterswerestudied.AnICD-9-basedCharlsonIndexwasusedtoadjustforunderlyingillness.Crudemortalitywas24%(14%forcommunity-onsetversus34%fornosocomialbloodstreaminfections).Mortalityattributedtothebloodstreaminfec...
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气相色谱法测定菝葜中薯蓣皂苷元含量
...器温度:300℃;载气流量:0.4L/min;分流比:20∶1;灵敏度:Range=10,att=1;进样量:4μl;纸速:3mm/min。2.2 样品溶液的制备精密称取干燥的菝葜药材适量,粉碎至中粉,加1.5mol/L盐酸水溶液在沸水浴中水解4h,过滤去除酸液,并将药材用水洗至中性,烘...