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GenomicScreeninginVivoRevealstheRolePlayedbyVacuolarH+ATPaseandCytosolicAcidificationinSensitivitytoDNA-DamagingAgentsSuchasCisplatin
...visiaehomozygousdiploiddeletionlibraryagainstasublethalconcentrationofcisplatinrevealed76strainssensitivetothedrug.Asexpected,thelargestcategoryofdeletions,representing40%ofthesensitivestrains,wascomposedofstrainslackinggenesinvolvedinDNAreplicationanddamagerepair.Deletionslackingfunctionofthehighly...
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Anti-inflammatoryeffectoffibrateprotectsfromcisplatin-inducedARF
...edthatperoxisomeproliferator-activatedreceptor-(PPAR)ligandamelioratescisplatin-inducedacuterenalfailure(ARF)bypreventinginhibitionofsubstrateoxidation,andalsobypreventingapoptosisandnecrosisoftheproximaltubule(LiS,BhattR,MegyesiJ,GokdenN,ShahSV,andPortillaD.AmJPhysiolRenalPhysiol287:F990F998,2004)....
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Cephalostatin1InactivatesBcl-2byHyperphosphorylationIndependentofM-PhaseArrestandDNADamage
...archInstitute,ArizonaStateUniversity,Tempe,Arizona(G.R.P.)AbstractCephalostatin1isamarineproductthatinducesanovelcytochromec-independentapoptoticpathwayinJurkatleukemiaTcells(CancerRes63:8869eC8876,2003).Here,weshowthatoverexpressionoftheantiapoptoticproteinBcl-2protectscellsonlypartiallyagainstceph...
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细胞骨架蛋白在肺的表达
...aldehydeandparaffinsectionwerepreparedaccoridngtoroutinemethods.Anticytokeratin-7,-8,-18,-19,antiactin,antivimentinandanti-alpha-tubulinmonochlonaeantibodies,wereemployedwithimmunohistochemicalABCmethed.Resalts:Theclaraceilsinterminalandrespiratorybronchioleswereimmunopositiveforcytokeratin-7,viment...
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Caspase-mediatedcleavageofATMduringcisplatin-inducedtubularcellapoptosis:inactivationofitskinaseactivitytowardp5
【摘要】Cisplatininducesrenalcellinjuryanddeath,resultinginnephrotoxicitythatlimitsitsuseincancertherapy.Usingcellculturemodels,recentworkhassuggestedtheinvolvementofp53inrenalcellapoptosisduringcisplatintreatment.However,thesignalsupstreamofp53remainelusive.ATMandATRarecriticalregulatorsofp53un...
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PROVEIT研究
PROVEIT研究PRavastatinOratorVastatinEvaluationandInfectionTherapy(TIMI22)DisclosureStatement:Dr.CannoncurrentlyreceivesresearchgrantsupportfromBristol-MyersSquibb,MerckandSanofi-Synthelabo.HeservesasaconsultanttoAstraZeneca,GlaxoSmithKline,GuilfordPharmaceuticalsandVertexBackgroundStatintherapyish...
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SuppressionofhREV1ExpressionReducestheRateatWhichHumanOvarianCarcinomaCellsAcquireResistancetoCisplatin
...oftheYfamilyofDNApolymerases.ToexaminetheroleofhREV1inthedevelopmentofcisplatin(DDP)resistance,asubline(2008-shREV1-3.3)oftheovariancarcinomacellline2008wasisolatedinwhichstableexpressionofashorthairpinRNAsuppressedhREV1expressionto20%andreducedhREV1proteinlevelto43%ofthatfoundintheparentalcells.The...
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EffectofLowDoseAtorvastatinVersusDiet-InducedCholesterolLoweringonAtheroscleroticLesionProgressionandInflammationinApolipoproteinE*3–LeidenT
...ilR.Kleemann@pg.tno.nlAbstractObjective—Toevaluatewhetherlow-doseatorvastatinsuppressesatheroscleroticlesionprogressionandinflammationinapolipoproteinE*3(apoE*3)–Leidenmicebeyonditscholesterol-loweringeffect.MethodsandResults—ApoE*3–Leidenmicewerefedahigh-cholesterol(HC)dietuntilmildatherosc...
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p38MAPkinaseinhibitionamelioratescisplatinnephrotoxicityinmice
...ersheyLebanonVeteransAffairsMedicalCenter,Lebanon,PennsylvaniaABSTRACTCisplatinisanimportantchemotherapeuticagentbutcancauseacuterenalinjury.Partofthisacuterenalinjuryismediatedthroughtumornecrosisfactor-(TNF-).ThepathwaythroughwhichcisplatinmediatestheproductionofTNF-andinjuryisnotknown.Cisplatinac...
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ThepublichealthimplicationsoftheDietaryApproachestoStopHypertensionTrial
...enewDietaryGuidelinesforAmericans(8).TheDASHdietandsimilardietarypatternsthatincludelow-fatdairyproductsandahighintakeoffruit,vegetables,andfiberprovideimportantguidelinesforpublichealthpolicy(9,10).Otherlifestylefactors—suchasphysicalactivitylevels,annualphysicalexams,immunizations,monitoringo...
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Hypoxia-induciblefactormodulatestubularcellsurvivalincisplatinnephrotoxicity
...ssachusettsABSTRACTHypoxia-induciblefactor(HIF)-1isatranscriptionfactormediatingcellularresponsetohypoxia.Althoughitisexpressedintubularcellsoftheischemickidney,itsfunctionalroleisnotfullyclarifiedinthepathologicalcontext.Inthisstudy,weinvestigatedaroleofHIFintubularcellapoptosisinducedbycisplatin.H...
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Bryostatin1andUCN-01Potentiate1--D-Arabinofuranosylcytosine-InducedApoptosisinHumanMyeloidLeukemiaCellsthroughDisparateMechanisms
...sultsDiscussionReferencesTheeffectsofthePKCactivatoranddown-regulatorbryostatin1andthePKCandChk1inhibitor7-hydroxystaurosporine(UCN-01)werecomparedwithrespecttopotentiationof1--D-arabinofuranosylcytosine(ara-C)-inducedapoptosisinhumanmyelomonocyticleukemiacells(U937).Whereasbryostatin1andUCN-01bothm...
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HMG-CoAReductaseInhibitorIncreasesGTPCyclohydrolaseImRNAandTetrahydrobiopterininVascularEndothelialCells
...vityissupportedbytetrahydrobiopterin(BH4),whichappearstobeimportantforgeneratingprotectiveNObutdecreasesuncouplingformationofsuperoxide.Weinvestigatedtheeffectsof3-hydroxy-3-methylglutarylcoenzymeAreductaseinhibitors,orstatins,intermsofBH4metabolisminhumanumbilicalveinendothelialcells(HUVECs).Method...
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他汀药物治疗ACS
...治疗ACS中南大学湘雅二医院心内科赵水平ScandinavianSimvastatinSurvivalStudy(4S)研究对象：冠心病合并高胆固醇血症患者4444名既往有心肌梗死或心绞痛史的男性或女性患者血清总胆固醇5.5-8.0mmol/L主要终点：总死亡率研究设计：多中心、...
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Pravastatintreatmentattenuatesinterstitialinflammationandfibrosisinaratmodelofchroniccyclosporine-inducednephropathy
【摘要】Weinvestigatedtheeffectsofpravastatin,acompetitiveinhibitorof3-hydroxy-3-methylglutaryl-coenzymeA(HMG-CoA)reductase,oninterstitialinflammationandfibrosis,usingananimalmodelofchroniccyclosporineA(CsA)-inducednephropathy.Sprague-Dawleyratsweremaintainedonalow-saltdiet(0.05%sodium)andtreate...
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运用荧光PCR进行大肠癌患者外周血中CK20表达的研究
...键词:大肠癌;荧光PCR;细胞角蛋白20Studyontheexpressionofcytokeratin20inperipheralbloodofcolorectoalcarcinomapatientsbyusingfluorescenceRT-PCR. GUOJian-ping，ZhangYang-de. （YuebeiPeople‘sHospitalofShaoguanCity，Shaoguan512025，Guangdong，P.R.Chi-na） Abstract:Objecti...
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继发真菌感染与应用抗生素的关系
...nourHospitalfromJanuary2005toDecember2007werestatisticallyanalysedbyyntegr-atingwithclinic.Results:Thefunguspatienusinfectionduetotheantibioticsholds65.6%(82/125),thesecondaryfungusinfectionpatientsoccurrdmainlytumorpatientsandgeriatricpatientswithpoorhealth,Theinfectionsitesliemostlyinlungsandurolo...
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ASystematicReviewandMeta-AnalysisofStatinTherapyinChildrenWithFamilialHypercholesterolemia
...isease.ThepurposeofthisstudywastoassesstheefficacyandparticularlysafetyofstatintherapyinchildrenwithHeFH.MethodsandResults-Weperformedameta-analysisofrandomized,double-blind,placebo-controlledtrialsevaluatingstatintherapyinchildrenaged8to18yearswithHeFH.Sixstudies(n=798children)with12to104weeksoftre...
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Stressresponseinhibitsthenephrotoxicityofcisplatin
...giniaABSTRACTSaltloadingandsalinehydrationareusedtoprotectpatientsfromcisplatin-inducednephrotoxicity.Themechanismbywhichsaltexertsitsprotectiveeffectisunknown.Aspartofanongoingstudyofcisplatinnephrotoxicity,aninvitroassaysystemwasdevelopedthatmodelstheinvivoexposureandresponseofproximaltubulecellst...
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RosuvastatinReducesPlateletActivationinHeartFailure
...s.AnimalswereeithertreatedwithplaceboortheHMG-CoAreductaseinhibitorrosuvastatin.After10weeks,hemodynamicassessmentwasperformedandendothelialfunctionwasdeterminedinorganbathstudies.NObioavailabilitywasassessedbyinvivoplateletvasodilator-stimulatedphosphoprotein(VASP)phosphorylation.Markersofplateletd...
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术后早期炎性肠梗阻的诊断与治疗
...lcuredthroughgastroenteraldecompression，totalparenteralnutritionso-matostatin，andothermethods.Theaveragecuredtimeis14days.ConclusionTheEPISBOareoftenoccurringinthetwoweeksafterabdominaloperation.Ithasthetypicalsignsandsymptomsofbowelobstructionbutrareintestinalis-chemia.Conservativetreatmentisth...
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ImmunomodulatoryEffectofCombinationTherapywithLovastatinand-Aminoimidazole--Carboxamide--ß-D-RibofuranosideAlleviatesNeurodegenerationinExpe
...erosis(MS)therapeuticsisgainingmomentumovermonotherapyforimprovingMS.Lovastatin,anHMG-CoAreductaseinhibitor(statin),wasimmunomodulatoryinanexperimentalautoimmuneencephalomyelitis(EAE)modelofMS.LovastatinbiasestheimmuneresponsefromTh1toaprotectiveTh2responseinEAEbyadifferentmechanismthan5-aminoimidaz...
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草酸铂加氟尿嘧啶及亚叶酸钙治疗晚期胃癌
...结论Oxa+5-FU+CF是治疗晚期胃癌安全有效的方法。 Oxaliplatin，fluorouracilandcalciumfolinateinthetreatmentofpatientswithadvancedstomachcancer LiuShujuan，ZhangZhengfu，ZhangXinyi InternalMedicinofTumorHospital，Qingdao，Shandong266042. 【Abstract】ObjectiveTo...
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EffectsofAllergenChallengeonAirwayEpithelialCellGeneExpression
...oattractants,proallergicinterleukins,matrix-modifyingproteins,andproteinsthatinfluencethegrowthandactivationstateofairwaystructuralcells.Theseproteins,inturn,contributetotheinfluxofinflammatorycellsandchangesinstructurethatcharacterizetheasthmaticairway.Tousetheresponseoftheairwayepitheliumtoallerge...
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前列通淤提取液对前列腺平滑肌细胞增殖与凋亡的作用
...下观察细胞形态变化，取第1、5代细胞行抗α-Actin、Cytoker-atin18免疫细胞化学染色，分别计算阳性细胞占总细胞数的百分比。 1.3MTT测定细胞抗增殖指数实验分7组，每组8个复孔，按细胞密度5×103/cm2接种于96孔培养板。加入含血...
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Protectionofrenalcellsfromcisplatintoxicitybycellcycleinhibitors
【摘要】Theoptimaluseofcisplatinasachemotherapeuticdrughasbeenlimitedbyitsnephrotoxicity.Murinemodelshavebeenusedtostudycisplatin-inducedacuterenalfailure.Aftercisplatinadministration,cellsoftheS3segmentintherenalproximaltubuleareespeciallysensitiveandundergoextensivenecrosisinvivo.Similarly,cul...
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PPAR-ligandamelioratesacuterenalfailurebyreducingcisplatin-inducedincreasedexpressionofrenalendonucleaseG
【摘要】Cisplatininjurytothekidneyischaracterized,inpart,byinhibitionofsubstrateoxidation,inflammation,andtubularcelldeathintheformofapoptosisandnecrosis.Recently,wedemonstratedthatcisplatin-inducedinhibitionofsubstrateoxidationcanbereversedbytheadministrationofperoxisomeproliferator-activatedre...
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Opticnerveoxygentension:theeffectsoftimololanddorzolamide
...hydraseinhibitors(CAIs)arealsowidelyusedforloweringintraocularpressureintreatingglaucoma,oftenusedincombinationwitha?blocker.2Atleastsomeformsofglaucomaareconsideredtohaveopticnerveischaemiaasanimportantpartoftheirpathogenesis.3,4Accordingly,significantresearcheffortshavebeendevotedtostudytheeffects...
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PPARligandprotectsduringcisplatin-inducedacuterenalfailurebypreventinginhibitionofrenalFAOandPDCactivity
【摘要】Previousstudiesdemonstratedthatduringcisplatin-inducedacuterenalfailure,thereisasignificantreductioninproximaltubulefattyacidoxidation.Wenowreportontheeffectsofperoxisomeproliferator-activatedreceptor-(PPAR)ligandWy-14643(WY)ontheabnormalitiesofmediumchainfattyacidoxidationandpyruvatedeh...
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Towardtranscriptionaltherapiesforthefailingheart:chemicalscreenstomodulategenes
...paniedbymyocytehypertrophy,impairedcontractility,andpumpfailure,oftenculminatinginsuddendeath.Theexistenceofredundantsignalingpathwaysthattriggerheartfailureposeschallengesfortherapeuticintervention.Cardiacremodelingisassociatedwiththeactivationofapathologicalgeneprogramthatweakenscardiacperformance...