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增加侧支循环的方法学研究概况
...好地反映动脉供血。采用超声电极可测得平均顶峰流速（APV,cm·s-1），静息APV为血流稳定后APV，最大APV为使用血管扩张剂后APV。超声血流量（QD,ml·min-1）测量需结合血管造影，QD=πd2(0.5×APV)/4,d为血管直径。由于药物（器粟碱）对...
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急性冠状动脉病变后微循环障碍发生时限及预处理对其影响
...状态与冠脉狭窄10min、30min、60min、120min时平均峰值流速(APV)以及舒张期与收缩期血流速度比值(DSVR)及冠脉血流储备(CFR)等指标。结果3组家猪冠脉狭窄10min时APV，DSVR，CFR较基础状态时明显下降(P＜0.05)，APV、DSVR在随后实验过程中无...
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Acuteunilateralureteraldistensioninhibitsglutamate-dependentspinalpelvic-urethrareflexpotentiationviaGABAergicneurotransmissioninanesthetizedra
...oxazoleproprionat(AMPA)receptorantagonist]andD-2-amino-5-phosphonovalerate[APV;aglutamatergicN-methyl-D-aspartate(NMDA)antagonist;thespikenumberperstimulation:11.0±0.70forNBQX,1.01±0.30forAPV,and16.90±2.0forRS,respectively,n=7,P0.01].AcutestepwiseelevationsofIUPgraduallyattenuat...
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TranscriptionalRegulationofBrain-DerivedNeurotrophicFactorintheAmygdaladuringConsolidationofFearMemory
...ver.ActinomycinD,anisomycin,TrkB/Fcchimera,D-2-amino-5-phosphonovalerate(D-APV)(Sigma,St.Louis,MO),verapamil,KT5720,KN62(Tocris,Bristol,UK),Rp-cAMP,water-solublemyristoylatedautocamtide-2-relatedinhibitorypeptide(BIOMOLResearchLaboratories,Ply-mouthMeeting,PA),water-solubleKN93,helenalin,and2-anti-p...
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激光治疗外阴尖锐湿疣96例分析
...方式的疾病，又称性病疣，主要病原体为人乳头瘤病毒（APV），它侵犯下生殖道鳞状上皮，好发于外阴部，系目前研究较多的宫颈癌病毒因子，不少文章报告宫颈鳞癌及腺癌、外阴癌及阴茎癌中发现APV结构蛋白，故认为此病毒有...
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SpinalglutamatergicNMDA-dependentpelvicnerve-to-externalurethrasphincterreflexpotentiationcausedbyamechanicalstimulationinanesthetizedrats
...cnerve-to-EUSreflexpotentiationwasabolishedbyD-2-amino-5-phosphonovalerate[APV;aglutamatergicN-methyl-D-asparticacid(NMDA)receptorantagonist;100µM,10µl,1.72±0.31spikes/stimulation]andattenuatedby2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline[NBQX;aglutamatergic-amino-3-hydr...
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双极胸导联对心肌梗塞的诊断价值如何？
...连接方法为：将左右上肢电极板移至胸部不同部位得出。APV：正极放至胸骨左缘第3肋间，负极放在左腋中线第4肋间，代表前间壁、前壁(V1～V5)；PPV：正极放至左腋中线第3肋间，负极放至胸骨右缘第2肋间，代表正后壁(V7～V9)；LP...
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ModulationofPresynaptic3-ContainingGABAAReceptorsLimitstheImmobilizingActionsofGABAergicAnesthetics
...quinoxaline-2,3-dione(CNQX;50µM),DL-2-amino-5-phosphonopentanoicacid(APV,50µM),and1µMstrychnine(allfromSigma).SignalswereacquiredwithanEPC7amplifier(ListMedical,Darmstadt,Germany),lowpass-filteredat3kHz,anddigitizedonapersonalcomputerat10kHzviaaDigidata1200interfaceandClampex(AxonI...
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Tetanization-inducedpelvic-to-pudendalreflexplasticityinanesthetizedrats
...enzo(F)quinoxaline(NBQX;20µM,Sigma),D-2-amino-5-phosphonoralericacid(APV100µM,Sigma),bicucullinemethiodide(10µM,Sigma),L-glutamate(0.1mM,Sigma),andNMDA(0.1mM,Sigma).Saline,ofidenticalvolumetotestedagents,wasdispensedintrathecallytoserveasavehicle.Attheendoftheexperiment,thelocation...
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NR2BSubunitExertsaCriticalRoleinPostischemicSynapticPlasticity
...dtherecordingchambernolaterthan30secondsaftera3-waytapwasturned.DrugswereD-APV,ifenprodil,NMDA(fromTocris-Cookson),andbicuculline(fromSigma).CP101,606wasakindgiftofDrVicini,GeorgetownUniversity,Washington,DC.ElectrophysiologicalIdentificationofIschemicPenumbraToassesstheischemicpenumbraafterinvivois...
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湖北省未经抗病毒治疗的HIV-1感染者的耐药基因变异分析
...价（表3）。可以看到，蛋白酶基因上的突变除K20M能引起APV和LPV的低度耐药突变外，均未能达到低度耐药的水平,在有其他主要耐药突变出现的情况下，L63位，A71位，V77位，I93位的突变对病毒的耐药性有一定的影响，但单独存在不...
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LowAccumulationofL90MinProteasefromSubtypeFHIV-1withResistancetoProteaseInhibitorsIsCausedbytheL89MPolymorphism
...e:saquinavir(SQV),ritonavir(RTV),indinavir(IDV),nelfinavir(NFV),amprenavir(APV),lopinavir(LPV),and,morerecently,atazanavir(ATV)[57].DespitethegreatpotencyofHAARTthatincludesatleast1PI,resistantstrainscanemergefromtheviralpopulationcirculatingintheinfectedindividualandcausetherapyfailure[811].Thesere...
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IncreasedExtracellularK+ConcentrationReducestheEfficacyofN-methyl-D-aspartateReceptorAntagoniststoBlockSpreadingDepression-LikeDepolarizations
...60minutes)inratneocorticalslicesperfusedwiththecompetitiveNMDARantagonist2-APV(n=6).ThefirstSD-likedepolarizationoccurredwhen[K+]oreached12.3±1.7mmol/L,characterizedbyanegativeDCshiftandatransientincreaseof[K+]o(Table3).OpticalchangesconsistedofasuddenLTdecreasesimultaneouslywiththeDCshift.Occasion...
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一氧化氮的生理作用
...张可被血红蛋白（Hb）和NMDA拮抗剂D-2-氨基-5-磷酰戊酸（APV）抑制，并证明谷氨酸和NMDA可显著刺激小脑薄片精氨酸的转化，并伴有cGMP水平增加，NOS抑制剂L-NMDA可阻断瓜氨酸和cGMP增加。Hb可阻止刺激cGMP水平增加，超氧化物歧化酶...
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老年病人腹腔镜全麻胃癌根治术中肺保护性通气的应用
...nesthesiaintubationand15minafterextubationandintraoperativeairwaypeakvalue(APV)wereobservedandtheresultswerecomparedamongthegroups.ResultsThirtyminutesafterintubation,theAPVofgroupAwassignificantlylowerthanthatofgroupsBandC(F=17.61;q=5.43-8.84;P<0.05),and15minutesafterextubation,thePaO2wassignifican...
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Lipoprotein-AssociatedPhospholipaseA2IsanIndependentMarkerforCoronaryEndothelialDysfunctioninHumans
...odflow(CBF)wasdeterminedas(coronaryarterydiameter/2)2x[averagepeakvelocity(APV)/2].ThevelocitysignalsareinstantaneouslyobtainedfromtheDopplerwirebyanonlinefastFouriertransform.Twopreviousstudieshavevalidatedthevelocitysignalsobtainedbythismethod,25,26andananalysisofdatafromthecurrentlaboratorydemons...
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NADPHOxidaseeCDerivedSuperoxideAnionMediatesAngiotensinIIeCInducedPressorEffectviaActivationofp38MitogeneCActivatedProteinKinaseintheRostral
...perfusate.ThesesEPSCsweretotallyblockedbycoapplicationofCNQX(20eol/L)plusD-APV(50eol/L)intheperfusate(datanotshown),confirmingthattheyareglutamatereceptor-mediatedsynapticevents.In5RVLMneuronstested,AngII(0.5eol/L)significantlyincreasedthemeanfrequencyofthesEPSCsfrom2.33±0.18to4.38±0.17Hz(Figure7A...
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RanGAP-MediatedNuclearProteinImportinVascularSmoothMuscleCellsIsAugmentedbyLysophosphatidylcholine
...cellcytoplasmincloseproximitytothenucleus.ThemicroinjectorusedinourstudywasaPV830PneumaticPicoPump(WorldPrecisionInstruments),andsettingswereasfollows:injectionholdpressure,40psi;ejectpressure,60psi;range,100ms.Cellswereinjectedfourtimes,andthepipettewasslowlyremoved.Imagesofthemicroinjectedcellwere...
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SpinalinflammatoryhyperalgesiaismediatedbyprostaglandinEreceptorsoftheEP2subtype
...-nitroquinoxaline-2,3-dione(CNQX;10μM),D-2-amino-5-phosphonovalericacid(D-APV;50μM),andbicuculline(10μM).Shorthyperpolarizingvoltagestepsto–90mVwereappliedin1-minuteintervalstomonitorinputandaccessresistance.PGE2(1μM)wasappliedbybathperfusionatarateof1–2ml/min.AcknowledgmentsThisworkwasinpar...
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RegulationofWAVEaMetastasisPromoterGeneInhibitsInvasionandMetastasisofBreastCancerCells
...erepresentedasmean??SE.TheStudent??st-testwasusedforthestatisticalanalysis.APvalueof<0.05wasconsideredsignificant.ResultsDown-RegulationofWAVE3withShortHairpinRNAsReducesInvasivenessofMDA-MB-231CellsWehavepreviouslyshownthattransienttransfectionofsiRNAoligonucleotidestoWAVE3effectivelyreducesWAVE3pr...
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Poly(ADP-ribose)polymerase-1geneablationprotectsmicefromischemicrenalinjury
...entalgroup.One-wayANOVAwithposttestwasusedtocomparemeanvaluesfromtwogroups.APvalueof<0.05wasconsideredasstatisticallysignificant.RESULTSToelucidatewhethertheabsenceofPARPactivityamelioratesthecourseofischemicrenalinjury,weinvestigatedtheeffectsofinducingrenalinjuryinwild-typeandPARP-deficientmice.Th...
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AldosteroneInducesElastinProductioninCardiacFibroblaststhroughActivationofInsulin-LikeGrowthFactor-IReceptorsinaMineralocorticoidReceptor-Ind
...hexperimentalgroup,andstatisticalanalyseswereperformedbyanalysisofvariance.APvalueoflessthan0.05wasconsideredsignificant.ResultsAldosteroneUp-RegulatesCollagenTypeIGeneExpressionandtheDepositionofCollagenFibersinanMR-DependentMannerinCulturesofCardiacFibroblastsWefirstdemonstratedthattreatmentofcult...
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HyperhomocysteinemiaandvitaminB-12deficiencyinelderlyusingTitleIIIcnutritionservices
...ategorieswereevaluatedwithattestandLevene’stestforequalityofvariances.APvalue0.05wasconsideredsignificant.Pairedttestswereusedtocomparethepre-andposttreatmentvariablesinthetreatmentgroup.Differencesincategoricalvariablesweretestedbyuseofthechi-squarestatistic.Multiplestepwiselogisticregressiona...
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InhibitionofIntrahepaticBileDuctDilationofthePolycysticKidneyRatwithaNovelTyrosineKinaseInhibitorGefitinib
...caldifferencesweredeterminedusingtheMann-WhitneyU-testoranalysisofvariance.APvalue<0.05wasacceptedasthelevelofstatisticalsignificance.ResultsEffectsofGefitinibonBiliaryCystFormationinVitroThethree-dimensionalcellculturesystemwasusedforthedeterminationoftheeffectsofgefitinibonbiliarycystformation.Whe...
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TheDietaryIsothiocyanateSulforaphaneIsanAntagonistoftheHumanSteroidandXenobioticNuclearReceptor
...ol,RIF-,orRU486-mediatedinductiveresponseinthedifferentculturedcellsystems.APvaluelessthan0.05wasconsideredtobesignificant.One-wayanalysisofvariancewasusedwhenmultiplecomparisonsweremade,followedbyDunnett‘sttestformultiplecomparisonstoacontrol.SFNEfficientlyInhibitsSXRActivity.Wepreviouslyobserved...
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GATA-6regulatessemaphorin3Candisrequiredincardiacneuralcrestforcardiovascularmorphogenesis
...N)Thereissingleoutflowtractvessel,orTA;asingle,commonaorticopulmonaryvalve(APV);andamembranousventricularseptaldefect(VSD).(JandO)RepresentativeWntCre+embryoexhibitingTAandVSD.Originalmagnification,x20(A–E);x40(F–O).Thecardiovascularmalformationsobservedrecapitulatedcommonformsofcongenitalheartd...
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CD40inducesmacrophageanti–Toxoplasmagondiiactivitybytriggeringautophagy-dependentfusionofpathogen-containingvacuolesandlysosomes
...doesnotoccurduringphagocytosisofparasites.ToconfirmthattachyzoitesresidedinaPV,experimentswereconductedwithtransgenicT.gondii,whichexpressafusionproteinconsistingofP30majorsurfaceantigenandasecretedredfluorescentprotein(DsRed;T.gondii–secRFP;theGPIanchorattachmentsignalwasdeletedtogenerateasoluble...