与Well手术相关的文献报道

改良式Gard-Well颅骨牵引弓的研制及临床应用

...，即改变为中立位牵引，并用2～3kg重量作维持牵引。1.3.3手术治疗在存在骨折和脱位的颈椎损伤患者中，在颅骨的牵引下，26例前路减压植骨融合，14例后路减压，20例采用牵引复位的保守疗法。2结果2.1单纯颅骨牵引颈椎复位法...

直肠脱垂

...，直肠脱垂是一种自限性疾病，可在5岁前自愈，故以非手术治疗为主。成人完全性直肠脱垂较严重的，长期脱垂将致阴部神经损伤产生肛门失禁、溃疡、肛周感染、直肠出血，脱垂肠段水肿、狭窄及坏死的危险，应以手术治疗...

经口吞入异物急诊处置体会

...据异物的大小、形状、位置等信息综合判断，采取胃镜、手术或保守治疗。②胃镜：文献报告95%的胃内异物均可通过胃镜取出。其中美国Well医生报告242例，239例经内镜取出，成功率98.8%。本组有9例由胃镜成功取出。③手术：通...

SpontaneousactivationoftheNF-Bsignalingpathwayinisolatednormalglomeruli

...tromelysinandMCP-1(11),whichwasprecededbyrapidupregulationofc-fosandc-junaswellasactivationofMAPkinasesincludingERK,p38MAPkinase,andJNK,withoutanyexternalstimuli(11).Theseresultsindicatedthat,immediatelyafterexplantation,MAPkinasepathwaysarespontaneouslyactivated,leadingtoconsequentinductionoftheAP-...

年龄及原发肿瘤大小对分化型甲状腺癌残留/复发危险的影响

...期不能作为预测肿瘤残留/复发风险，而肿瘤组织在初始手术时的甲状腺外侵袭可以作为预测因素。这是来自加拿大的研究人员进行的一项回溯性的研究，共有246例WDTC患者纳入研究，结果显示246例患者平均生存5.8年，年龄、原发...

RNA干扰survivin基因对HepG2细胞凋亡及化疗影响

...shRNAvectorspshRNAsurvivin387wereconstructed.HepG2cellsculturedin6wellculturedishweredividedintofivegroups:blankcontrolgroup(withoutanydrug),negativecontrolgroup(3.2μgpshRNAsurvivinNC3/well),lowdosegroup(0.8μgpshRNAsurvivin387/well),mediumdosegroup(1.6μgpshRNAs...

“我想买消炎痛”

...为非麻醉性止痛药，主要用于中等程度的各种急性疼痛及手术后疼痛等；还有一种是麻醉性止痛药，以吗啡、杜冷丁等阿片类药为代表。这类药物止痛作用很强，但反复使用会成瘾。　　Shopassistant:Therearethreetypesofanalgesics,eachusedi...

鼻内镜手术治疗鼻窦真菌病

【摘要】目的讨论鼻内镜手术治疗鼻窦真菌病的疗效。方法对27例鼻窦真菌病患者施行鼻内镜手术，术后不用抗真菌药。结果全部患者均获治愈，随访6个月～3年未见复发。结论在本病的治疗中，应用鼻内镜手术可减轻对患者的...

δ-榄香烯体外抗肿瘤活性研究

...CF-7用0.25%的胰蛋白酶消化，以5×104/ml接种于96孔板（100μl/well），并于5%CO237℃培养过夜后，加入不同浓度的δ-榄香烯（0、50、100、200、400μM）100μl/well；同时设空白对照组（0μMδ-榄香烯），复设3孔。药物作用12，24，48h后，弃培...

LeucinerequirementandsplanchnicuptakeofleucineinchronicallyundernourishedadultIndiansubjects

...ouslybythe24-hdirectaminoacidbalance(DAAB)methodthattheleucinerequirementofwell-nourishedWesternandSouthAsiansubjectsis40mg•kg-1•d-1.Objective:Itisnotknownwhetherthisestimatedleucinerequirementisapplicableinchronicundernutrition;therefore,weassessedtheleucinerequirementinIndianmenwithchron...

LactobacillusrhamnosusinducesperipheralhyporesponsivenessinstimulatedCD4+Tcellsviamodulationofdendriticcellfunction

...alallergicdisease(7,8)suggestthatadirecteffectontheimmunesystemisinvolvedaswell.BecauseinflammatoryboweldiseasedisplaysmainlyThelpersubset1(Th1)characteristicsandbecausetopicalallergiesarecharacterizedbyaThelpersubset2(Th2)phenotype,bothbenefitfromprobioticsupplementation(15);theimmunologiceffectsof...

细胞凋亡的检测

...1.用包被Buffer稀释的重组人TFAR19（1mg/ml）包被ELISA板,100ml/well,37°C孵育2h或4°C过夜（一般24h以上）。　　2.洗涤Buffer洗板三次，加入封闭液，200ml/well，37°C孵育2h或4°C过夜。　　3.洗涤Buffer洗板三次，加入不同稀释度的病...

ImmunomodulatorFTY720InduceseNOS-DependentArterialVasodilatationviatheLysophospholipidReceptorS1P3

...lemiceandwild-type(WT)controls(CharlesRiverLaboratories,Wilmington,Mass),aswellasS1P3-nullmiceandWTcontrols.21,22Thewalltensionofthevasculaturewasmeasuredinmiceaortaeusingestablishedmethodology.23,24Arterialcontractionstudieswereperformedandpresentedasequilibrationdose-responsecurvesforphenylephrine...

二次剖宫产术152例临床分析

...剖宫产史者，另一组为常规子宫下段剖宫产史者。两组在手术开始至胎儿娩出时间、手术总时间、术中出血量、腹腔粘连状况、子宫切口愈合情况、术后排气时间、术后病率、术后拆线时间方面进行分析和比较。结果简化剖宫产...

NKcellspromotetransplanttolerancebykillingdonorantigen-presentingcells

...epresenceoflargenumbersofdonorAPCsnormallyresidingintransplantedorgans.ItiswellknownthatprimingbythedirectpathwayoftenactivatesanunusuallylargemassofalloreactiveTcellclones,whichconstitutesoneofthepotentbarrierstotheinductionoftransplanttolerance(11).Asthepresenceofhostsecondarylymphoidorgans(e.g.,s...

螺旋CT和三维重建技术在三踝骨折治疗中的应用

...义。［方法］回顾性分析1999～2004年56例三踝骨折病例，手术前均行X线正侧位摄片，螺旋CT的扫描参数为厚层2～5mm，床速3～5mm，重建间距15～3mm，而后行螺旋CT三维重建技术并与手术对照。［结果］所有病例螺旋CT三维重建技术...

Effectofzincsupplementationonimmuneandinflammatoryresponsesinpediatricpatientswithshigellosis

...nc)werecoatedwithantisecretorycomponent(1:2000incarbonatebuffer,100µL/well;DakopattsAB,Denmark),horseradishperoxidase-conjugatedantisecretorycomponent(1:1000;Dako)wasusedasaconjugate,andtheplatesweredevelopedbyusingorthophenylenediamine(Sigma,StLouis)asthesubstrate.Measurementofsolubleinnatemed...

肩上方悬吊复合体联合损伤的治疗体会

...7例SSSC联合损伤病人的临床资料，2例行保守治疗，其余行手术治疗。手术治疗组中4例仅固定明显移位或脱位的锁骨或肩锁关节，11例于损伤处行联合固定。结果平均随访17.5个月,所有骨折均愈合。保守治疗组ASES评分67.1分，手术...

Mannan-bindinglectinactivatesC3andthealternativecomplementpathwaywithoutinvolvementofC2

...itionssuchasC4andC2deficiency.HomozygousdeficienciesofC4andC2aregeneticallywell-definedimmunodeficiencystatesthatpredisposetoinfectionanddevelopmentofimmunologicaldisease(16,17).C2deficiencyhasanestimatedprevalenceofabout1in20,000inWesternpopulations(18).CompleteC2deficiency,C2deficiencytypeI,isusua...

ThenewhomefortheJCIis...

...waysthoughteditingajournalwouldbefun—butIwouldonlydoitforajournalIfeltwaswellrespectedinthefieldandonethatIreallyenjoyedreading.IthoughtthiswouldbesomethingIcouldcontributeto,anditisanopportunitytoworkwithanexcellentsetofcolleagueshere.JCI:Howdidyouchoosethedeputyeditors?Turka:Italkedtoseveralofth...

细胞培养支原体检测

...成细胞悬浮液，以1~2x10^4cells／ml培养于chamberslide中，每个well加入1ml细胞悬浮液，于37℃,5％CO2培养。隔日确定生长良好后，即可接种测试样品。　　·接种测试样品：样品种类：1ml待测之培养基，1ml待测细胞培养液（可将细胞稍...

AntitumorActivityofMethoxymorpholinylDoxorubicin:PotentiationbyCytochromeP4503AMetabolism

...ngetal.,1997).MicrosomesProdrugCocultureAssay.Tumorcelllineswereseededin96-wellmicroplatesatadensityof1000cells/well(9L,A549,U251,CHO-hP450R,andCHO-3A4-hP450Rcells)or3000cells/well(MCF7,MCF7-ADR,andLS180cells)andwereallowedtoattachfor24h.MMDXdilutedinfreshculturemediumwasthenaddedtoeachwellatconcent...

AWaterborneOutbreakofNorwalk-LikeVirusamongSnowmobilersWyoming,2001

...ndillness,andtestingidentifiedNorwalk-likevirus(NLV)in8of13stoolsamplesand1well.Nucleotidesequencesfromthepositivewell-waterspecimenand6ofthepositivestoolsampleswereidentical.ThismultistrainNLVoutbreakinvestigationillustratestheimportanceofNLVasacauseofwaterborneillnessandshouldencouragemonitoringfo...

UnnaturalAminoAcid-Substituted(Hydroxyethyl)ureaPeptidomimeticsInhibit-SecretaseandPromotetheNeuronalDifferentiationofNeuroblastomaCells

...T16orT20cellsweretrypsinizedandwashedwithserum-freeDMEMbeforeplatingonto12-wellmicroplatesin1ml/wellDMEMsupplementedwith10%FBSat5x105cells/well.Afterincubationat37°Covernight,cellsweretreatedwith10µMconcentrationsofindividual(hydroxyethyl)ureapeptidomimeticsinculturemediumcontaining1%DMSOa...

MatrixMetalloproteinase-2and-9DifferentiallyRegulateSmoothMuscleCellMigrationandCell-MediatedCollagenOrganization

...ngconcentrations)inserum-freeDMEMwasaddedtoquiesced,confluentSMCseededin96-welltissuecultureplates.Afterwashingcollagenmonomersunboundafter24hours,SMCmonolayersandassociatedassembledcollagenwerefixed.NucleiwerecounterstainedwithHoechst.FluorescencewasmeasuredusingaCytofluor3000(AppliedBiosystems)wit...

AgonistsofToll-likeReceptors2and4ActivateAirwaySmoothMuscleviaMononuclearLeukocytes

...cturer‘smedia.TheywereusedwhenfullyconfluentbetweenPassages2through7in24-wellplatesandafter48hours‘starvationinserum-freemedia.LowconcentrationsofserumwereaddedduringstimulationwithTLRagoniststofacilitateLPSresponsivenessofASMorleukocytes;similarresultswereseenwithafinalconcentrationoffetalcalfs...

微创逆行带锁髓内钉治疗股骨中下段骨折

...引3～7天。　　图1右侧股骨中下段骨折术前（略）　　1.2手术方法硬膜外麻醉成功后，消毒铺巾，取仰卧位。膝下放自制木墩（已消毒并用厚软垫包裹），屈膝45°～60°方便复位及手术操作。取髌下长约4cm小纵切口，纵形劈开髌...

Glibenclamidepreventsincreasedextracellularmatrixformationinducedbyhighglucoseconcentrationinmesangialcells

...oteins(ARPP)(14,40).Thestructureof-endosulfine,encodedbytheENSAgene,hasbeenwellcharacterizedandfoundtobeubiquitouslyexpressedinmanytissues(25).Although-endosulfinehasbeenidentifiedasaputativeendogenousligandforKATPchannels,itsphysiologicalroleremainslargelyunknown.Beyondtheiractionsoninsulinsecretio...

股骨干骨折并同侧髋臼骨折的治疗

...均采用开放复位内固定。结果依据髋关节功能评价标准，手术3年内功能评价优61例，良18例，可5例，差2例，优良率达92％。结论依据患者不同骨折类型选用不同复位方法效果显著。［关键词］股骨干骨折；髋臼骨折；固定术Treatm...

DailymethioninerequirementsofhealthyIndianmen,measuredbya24-hindicatoraminoacidoxidationandbalancetechnique

...8,21,and24mg•kg-1•d-1),weassessedmethioninerequirementsinhealthy,well-nourishedIndians.Design:Twenty-onehealthy,well-nourishedIndianmenwerestudiedduringeachof3randomlyassigned7-ddietperiodsinwhichmethionineintakes(dietdevoidofcysteine)wereequallyplacedoneithersideoftheputativemeanmethionin...